Characterization of a new GmFAD3A allele in Brazilian CS303TNKCA soybean cultivar
We molecularly characterized a new mutation in the GmFAD3A gene associated with low linolenic content in the Brazilian soybean cultivar CS303TNKCA and developed a molecular marker to select this mutation.
Soybean is one of the most important crops cultivated worldwide. Soybean oil has 13% palmitic acid, 4% stearic acid, 20% oleic acid, 55% linoleic acid and 8% linolenic acid. Breeding programs are developing varieties with high oleic and low polyunsaturated fatty acids (linoleic and linolenic) to improve the oil oxidative stability and make the varieties more attractive for the soy industry. The main goal of this study was to characterize the low linoleic acid trait in CS303TNKCA cultivar. We sequenced CS303TNKCA GmFAD3A, GmFAD3B and GmFAD3C genes and identified an adenine point deletion in the GmFAD3A exon 5 (delA). This alteration creates a premature stop codon, leading to a truncated protein with just 207 residues that result in a non-functional enzyme. Analysis of enzymatic activity by heterologous expression in yeast support delA as the cause of low linolenic acid content in CS303TNKCA. Thus, we developed a TaqMan genotyping assay to associate delA with low linolenic acid content in segregating populations. Lines homozygous for delA had a linolenic acid content of 3.3 to 4.4%, and the variation at this locus accounted for 50.83 to 73.70% of the phenotypic variation. This molecular marker is a new tool to introgress the low linolenic acid trait into elite soybean cultivars and can be used to combine with high oleic trait markers to produce soybean with enhanced economic value. The advantage of using CS303TNKCA compared to other lines available in the literature is that this cultivar has good agronomic characteristics and is adapted to Brazilian conditions.
This work was funded by the CNPq (Grant 455812/2014-4), graduate fellowships (L.C.C.S. and L.B.M), post-doctoral fellowships (R.D.B) and a science initiation scholarship (D.B.M. and P.H.S.P.); FAPEMIG (Grant APQ-00077-13 and in part by APQ-01416-16). In memoriam professor Maurilio Alves Moreira.
Compliance with ethical standards
Conflict of interest
On behalf of all authors, the corresponding author declares that there is no conflict of interest.
- ABIOVE (2017) Estatística Mensal do Complexo SojaGoogle Scholar
- Artimo P, Jonnalagedda M, Arnold K, Baratin D, Csardi G, de Castro E, Duvaud S, Flegel V, Fortier A, Gasteiger E, Grosdidier A, Hernandez C, Ioannidis V, Kuznetsov D, Liechti R, Moretti S, Mostaguir K, Redaschi N, Rossier G, Xenarios I, Stockinger H (2012) ExPASy: SIB bioinformatics resource portal. Nucl Acids Res 40:W597–603CrossRefPubMedPubMedCentralGoogle Scholar
- Brazil (2017) Lei nº 13.263, de março de 2016Google Scholar
- Bruner AC, Jung S, Abbott AG, Powell GL (2001) The naturally occurring high oleate oil character in some peanut varieties results from reduced oleoyl-PC desaturase activity from mutation of aspartate 150 to asparagine. This work was partially supported by grants from the South Carolina Exp. Stn., Clemson Univ., and by a grant from AgraTech Seeds Inc., Ashburn, GA. Crop Sci 41:522–526Google Scholar
- CONAB (2017) Acompanhamento da Safra Brasileira. SAFRA, p 164Google Scholar
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
- FDA (2015) Final determination regarding partially hydrogenated oils (removing trans fat)Google Scholar
- FDA (2016) Constituent update: FDA takes step to remove artificial trans fats from processed foodsGoogle Scholar
- Fehr WR, Hammond EG (2000) Reduced linolenic acid production in soybeans. U. S. Patent 6,133,509Google Scholar
- Hammond EG, Fehr WR (1983) Registration of A5 germplasm line of soybean (Reg. No. GP44). Crop Sci 23:192–193Google Scholar
- Le DT, Aldrich DL, Valliyodan B, Watanabe Y, Ha CV, Nishiyama R, Guttikonda SK, Quach TN, Gutierrez-Gonzalez JJ, Tran LS, Nguyen HT (2012) Evaluation of candidate reference genes for normalization of quantitative RT-PCR in soybean tissues under various abiotic stress conditions. PLoS ONE 7:e46487CrossRefPubMedPubMedCentralGoogle Scholar
- Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, Hyten DL, Song Q, Thelen JJ, Cheng J, Xu D, Hellsten U, May GD, Yu Y, Sakurai T, Umezawa T, Bhattacharyya MK, Sandhu D, Valliyodan B, Lindquist E, Peto M, Grant D, Shu S, Goodstein D, Barry K, Futrell-Griggs M, Abernathy B, Du J, Tian Z, Zhu L, Gill N, Joshi T, Libault M, Sethuraman A, Zhang XC, Shinozaki K, Nguyen HT, Wing RA, Cregan P, Specht J, Grimwood J, Rokhsar D, Stacey G, Shoemaker RC, Jackson SA (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178–183CrossRefPubMedGoogle Scholar
- Thompson JD, Gibson TJ, Higgins DG (2002) Multiple sequence alignment using ClustalW and ClustalX. Curr Protoc Bioinform. Chapter 2: Unit 2–3Google Scholar
- USDA (2017a) Foreign agricultural service: table 03: major vegetable oils: world supply and distribution (commodity view)Google Scholar
- USDA (2017b) Foreign agricultural service: world agricultural productionGoogle Scholar
- Yadav NS (1996) Soybean genetics, molecular biology and biotechnology. Biotechnology in Agriculture Series. CAB InternationalGoogle Scholar