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Characterization of a new GmFAD3A allele in Brazilian CS303TNKCA soybean cultivar

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Abstract

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We molecularly characterized a new mutation in the GmFAD3A gene associated with low linolenic content in the Brazilian soybean cultivar CS303TNKCA and developed a molecular marker to select this mutation.

Abstract

Soybean is one of the most important crops cultivated worldwide. Soybean oil has 13% palmitic acid, 4% stearic acid, 20% oleic acid, 55% linoleic acid and 8% linolenic acid. Breeding programs are developing varieties with high oleic and low polyunsaturated fatty acids (linoleic and linolenic) to improve the oil oxidative stability and make the varieties more attractive for the soy industry. The main goal of this study was to characterize the low linoleic acid trait in CS303TNKCA cultivar. We sequenced CS303TNKCA GmFAD3A, GmFAD3B and GmFAD3C genes and identified an adenine point deletion in the GmFAD3A exon 5 (delA). This alteration creates a premature stop codon, leading to a truncated protein with just 207 residues that result in a non-functional enzyme. Analysis of enzymatic activity by heterologous expression in yeast support delA as the cause of low linolenic acid content in CS303TNKCA. Thus, we developed a TaqMan genotyping assay to associate delA with low linolenic acid content in segregating populations. Lines homozygous for delA had a linolenic acid content of 3.3 to 4.4%, and the variation at this locus accounted for 50.83 to 73.70% of the phenotypic variation. This molecular marker is a new tool to introgress the low linolenic acid trait into elite soybean cultivars and can be used to combine with high oleic trait markers to produce soybean with enhanced economic value. The advantage of using CS303TNKCA compared to other lines available in the literature is that this cultivar has good agronomic characteristics and is adapted to Brazilian conditions.

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Acknowledgements

This work was funded by the CNPq (Grant 455812/2014-4), graduate fellowships (L.C.C.S. and L.B.M), post-doctoral fellowships (R.D.B) and a science initiation scholarship (D.B.M. and P.H.S.P.); FAPEMIG (Grant APQ-00077-13 and in part by APQ-01416-16). In memoriam professor Maurilio Alves Moreira.

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  1. Andrea J. Cardinal

    Present address: Syngenta Biotechnology, Inc, 3054 Cornwallis Rd., Research Triangle Park, NC, 27709, USA

  2. Luiz Claudio Costa Silva, Rafael Delmond Bueno, and Loreta Buuda da Matta have contributed equally to this work.

Authors and Affiliations

  1. Laboratório de Bioquímica Genética de Plantas, 212, BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Luiz Claudio Costa Silva, Rafael Delmond Bueno, Danyelle Barbosa Mayrink & Newton Deniz Piovesan

  2. Seeds Bayer Crop Science, Rodovia GO 174, KM 11, Rio Verde, GO, Brazil

    Loreta Buuda da Matta

  3. Laboratório de Biologia Celular e Molecular de Plasmódio, Universidade de São Paulo, São Paulo, SP, 05508-900, Brazil

    Pedro Henrique Scarpelli Pereira

  4. Departamento de Fitotecnia, sala 205, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Carlos Sigueyuki Sediyama

  5. Laboratório de Biologia Molecular de Plantas, Sala 214, BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Elizabeth Pacheco Batista Fontes

  6. Crop Science Department, North Carollina State University, Raleigh, NC, 27695, USA

    Andrea J. Cardinal

  7. Laboratório de Bioquímica Genética de Plantas, 212, BIOAGRO and Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Maximiller Dal-Bianco

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  1. Luiz Claudio Costa Silva
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Silva, L.C.C., Bueno, R.D., da Matta, L.B. et al. Characterization of a new GmFAD3A allele in Brazilian CS303TNKCA soybean cultivar. Theor Appl Genet 131, 1099–1110 (2018). https://doi.org/10.1007/s00122-018-3061-2

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