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Cytogenetic mapping of a major locus for resistance to Fusarium head blight and crown rot of wheat on Thinopyrum elongatum 7EL and its pyramiding with valuable genes from a Th. ponticum homoeologous arm onto bread wheat 7DL

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A major locus for resistance to different Fusarium diseases was mapped to the most distal end of Th. elongatum 7EL and pyramided with Th. ponticum beneficial genes onto wheat 7DL.

Abstract

Perennial Triticeae species of the Thinopyrum genus are among the richest sources of valuable genes/QTL for wheat improvement. One notable and yet unexploited attribute is the exceptionally effective resistance to a major wheat disease worldwide, Fusarium head blight, associated with the long arm of Thinopyrum elongatum chromosome 7E (7EL). We targeted the transfer of the temporarily designated Fhb-7EL locus into bread wheat, pyramiding it with a Th. ponticum 7el1L segment stably inserted into the 7DL arm of wheat line T4. Desirable genes/QTL mapped along the T4 7el1L segment determine resistance to wheat rusts (Lr19, Sr25) and enhancement of yield-related traits. Mapping of the Fhb-7EL QTL, prerequisite for successful pyramiding, was established here on the basis of a bioassay with Fusarium graminearum of different 7EL-7el1L bread wheat recombinant lines. These were obtained without resorting to any genetic pairing promotion, but relying on the close 7EL-7el1L homoeology, resulting in 20% pairing frequency between the two arms. Fhb-7EL resided in the telomeric portion and resistant recombinants could be isolated with useful combinations of more proximally located 7el1L genes/QTL. The transferred Fhb-7EL locus was shown to reduce disease severity and fungal biomass in grains of infected recombinants by over 95%. The same Fhb-7EL was, for the first time, proved to be effective also against F. culmorum and F. pseudograminearum, predominant agents of crown rot. Prebreeding lines possessing a suitable 7EL-7el1L gene/QTL assembly showed very promising yield performance in preliminary field tests.

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Acknowledgements

This work and paper is dedicated to our highly esteemed colleague and dear friend Renato D’Ovidio, who prematurely passed away on March 2, 2017. We are all indebted to him for continuous inspiration, support, and enthusiasm, generously offered throughout his life, both at work and outside. Financial support from Lazio region—FILAS project “MIGLIORA” is gratefully acknowledged. This research is supported by the Premier’s Research and Industry Fund grant provided by the South Australian Government Department of State Development. We are thankful to Moshe Feldman for having provided seeds of the CS7E(7D) substitution line, to Douglas Knott and Robert McIntosh for seeds of KS lines and Agatha mutants, and to Penny Tricker for critical reading of the manuscript. Special thanks are due to Alessandra Bitti for excellent technical assistance.

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Authors and Affiliations

  1. Department of Agricultural and Forest Sciences (DAFNE), University of Tuscia, 01100, Viterbo, Italy

    Carla Ceoloni, Paola Forte, Ljiljana Kuzmanović, Silvio Tundo, Ilaria Moscetti, Maria Elena Virili & Renato D’Ovidio

  2. CREA-CER Cereal Research Centre, 71122, Foggia, Italy

    Pasquale De Vita

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  1. Carla Ceoloni
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  2. Paola Forte
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  3. Ljiljana Kuzmanović
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  4. Silvio Tundo
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  5. Ilaria Moscetti
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  6. Pasquale De Vita
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  7. Maria Elena Virili
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  8. Renato D’Ovidio
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Correspondence to Carla Ceoloni.

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Communicated by Steven S. Xu.

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Ceoloni, C., Forte, P., Kuzmanović, L. et al. Cytogenetic mapping of a major locus for resistance to Fusarium head blight and crown rot of wheat on Thinopyrum elongatum 7EL and its pyramiding with valuable genes from a Th. ponticum homoeologous arm onto bread wheat 7DL. Theor Appl Genet 130, 2005–2024 (2017). https://doi.org/10.1007/s00122-017-2939-8

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