Three novel QTL for peroxidase activity were mapped, and gene-specific markers for TaPod-A1 were developed and validated using RILs derived from the Doumai/Shi 4185 cross and 281 wheat cultivars. TaPod-A1 is within one of the three QTL.
Peroxidase (POD) activity in grain is an important factor determining the color of flour and end-use products of wheat, such as noodles and steamed bread. Mapping QTL for POD activity, characterization of POD genes and development of gene-specific markers are important for molecular marker-assisted selection in wheat breeding. Quantitative trait loci (QTL) for POD activity in common wheat were mapped using a recombinant inbred line (RIL) population derived from a Doumai/Shi 4185 cross grown in four environments and genotyped using the wheat 90 K iSelect assay. Three novel QTL for POD activity, QPod.caas-3AL, QPod.caas-4BS and QPod.caas-5AS, were identified on chromosomes 3AL, 4BS and 5AS, explaining 5.3–21.2 % of phenotypic variance across environments. The full-length genomic DNA (gDNA) sequence of a POD gene, designated TaPod-A1, on chromosome 3A was characterized by homolog cloning and PCR verification. Two complementary dominant sequence-tagged site (STS) markers, POD-3A1 and POD-3A2, were developed based on single nucleotide polymorphisms (SNPs) between two alleles at the TaPod-A1 locus, amplifying 291- and 766-bp fragments in cultivars with lower and higher POD activities, respectively. The two gene-specific markers were mapped on chromosome 3AL using a set of Chinese Spring (CS) nulli-tetrasomic lines, and ditelosomic lines 3AL and 3AS. QTL analysis indicated that QPod.caas-3AL co-segregated with the gene-specific markers POD-3A1 and POD-3A2. POD-3A1 and POD-3A2 were verified on 281 wheat cultivars and advanced lines, and showed significant (P < 0.05) associations with POD activities. POD-3A1 and POD-3A2 may be useful as markers for improving color attributes in wheat breeding programs.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Analysis of variance
Logarithm of odds
Open reading frame
Polymerase chain reaction
Quantitative trait loci/locus
Recombinant inbred line
Single nucleotide polymorphism
Bérard A, Le Paslier MC, Dardevet M, Exbrayat-Vinson F, Bonnin I, Cenci A, Haudry A, Brunel D, Ravel C (2009) High-throughput single nucleotide polymorphism genotyping in wheat (Triticum spp.). Plant Biotechnol J 7:364–374
Blee KA, Jupe SC, Richard G, Zimmerlin A, Davies DR, Paul Bolwell G (2001) Molecular identification and expression of the peroxidase responsible for the oxidative burst in French bean (Phaseolus vulgaris L.) and related members of the gene family. Plant Mol Biol 47:607–620
Boisson M, Mondon K, Torney V, Nicot N, Laine AL, Bahrman N, Gouy A, Daniel-Vedele F, Hirel B, Sourdille P, Dardevet M, Ravel C, Gouis L (2005) Partial sequences of nitrogen metabolism genes in hexaploid wheat. Theor Appl Genet 110:932–940
Borrelli GM, Troccoli A, Di Fonzo N, Fares C (1999) Durum wheat lipoxygenase activity and other quality parameters that affect pasta colour. Cereal Chem 76:335–340
Borrelli GM, De Leonardis AM, Fares C, Platani C, Di Fonzo N (2003) Effect of modified processing conditions on oxidative properties of semolina dough and pasta. Cereal Chem 80:225–231
Bosch A, Vega C, Benito C (1987) The peroxidase isozymes of the wheat kernel: tissue and substrate specificity and their chromosomal location. Theor Appl Genet 73:701–706
Brenchley R, Spannagl M, Pfeifer M, Barker GLA, D’Amore R, Allen AM, McKenzie N, Kramer M, Kerhornou A, Bolser D, Kay S, Waite D, Trick M, Bancroft I, Gu Y, Huo N, Luo MC, Sehgal S, Gill B, Kianian S, Anderson O, Kersey P, Dvorak J, McCombie WR, Hall A, Mayer KFX, Edwards KJ, Bevan MW, Hall N (2012) Analysis of the bread wheat genome using whole-genome shotgun sequencing. Nature 491:705–710
Feillet P, Autran JC, Icard-Vernière C (2000) Mini review pasta brownness: an assessment. J Cereal Sci 32:215–233
Fraignier MP, Michaux-Ferrière N, Kobrehel K (2000) Distribution of peroxidase in durum wheat (Triticum durum). Cereal Chem 77:11–17
Fry SC (1986) Cross-linking of matrix polymers in the growing cell walls of angiosperms. Annu Rev Plant Physiol 37:165–186
Gélinas P, Poitras E, McKinnon CM, Morin A (1998) Oxido-reductases and lipases as dough-bleaching agents. Cereal Chem 75:810–814
Geng HW, Xia XC, Zhang LP, Qu YY, He ZH (2012) Development of functional markers for a lipoxygenase gene TaLox-B1 on chromosome 4BS in common wheat. Crop Sci 52:568–576
Giroux MJ, Morris CF (1997) A glycine to serine change in puroindoline b is associated with wheat grain hardness and low levels of starch-surface friabilin. Theor Appl Genet 95:857–864
Goodin DB, McRee DE (1993) The Asp–His–iron triad of cytochrome c peroxidase controls the reduction potential electronic structure, and coupling of the tryptophan free radical to the heme. Biochemistry 32:3313–3324
Guillaumie S, Charmet G, Linossier L, Torney V, Robert N, Ravel C (2004) Colocation between a gene coding for the bZip factor SPA and an eQTL for a high-molecular-weight glutenin subunit in wheat (Triticum aestivum). Genome 47:705–713
Hemalatha MS, Manu BT, Bhagwat SG, Leelavathi K, Prasada Rao UJS (2007) Protein characteristics and peroxidase activities of different Indian wheat varieties and their relationship to chapatti-making quality. Eur Food Res Technol 225:463–471
Hessler TG, Thomson MJ, Benscher D, Nachit MM, Sorrells ME (2002) Association of a lipoxygenase locus, Lpx-B1, with variation in lipoxygenase activity in durum wheat seeds. Crop Sci 42:1695–1700
Hiraga S, Sasaki K, Ito H, Ohashi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol 42:462–468
Icard-Vernière C, Feillet P (1999) Effects of mixing conditions on pasta dough development and biochemical changes. Cereal Chem 76:558–565
Jia JZ, Zhao SC, Kong XY, Li YR, Zhao GY et al (2013) Aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation. Nature 496:91–95
Johansson A, Rasmussen SK, Harthill JE, Welinder KG (1992) cDNA, amino acid and carbohydrate sequence of barley seed-specific peroxidase BP 1. Plant Mol Biol 18:1151–1161
Kobrehel K, Feillet P (1975) Identification of genomes and chromosomes involved in peroxidase synthesis of wheat seeds. Can J Bot 53:2336–2344
Kobrehel K, Laignelet B, Feillet P (1974) Study of some factors of macaroni brownness. Cereal Chem 51:675–684
Kosambi DD (1944) The estimation of map distance from recombination values. Ann Eugen 12:172–175
Lagudah ES, Appels R, McNeil D (1991) The Nor-D3 locus of Triticum tauschii: natural variation and genetic linkage to markers in chromosome 5. Genome 34:387–395
Li WL, Faris JD, Chittoor JM, Leach JE, Hulbert SH, Liu DJ, Chen PD, Gill BS (1999) Genomic mapping of defense response genes in wheat. Theor Appl Genet 98:226–233
Li H, Ye G, Wang J (2007) A modified algorithm for the improvement of composite interval mapping. Genetics 175:361–374
Ling HQ, Zhao SC, Liu DC, Wang JY, Sun H, Zhang C, Fan HJ, Li D, Dong LL, Tao Y, Gao C, Wu HL, Li YW, Cui Y, Guo XS, Zheng SS, Wang B, Yu K, Liang QS, Yang WL, Lou XY, Chen J, Feng MJ, Jian JB, Zhang XF, Luo GB, Jiang Y, Liu JJ, Wang ZB, Sha YH, Zhang BR, Wu HJ, Tang DZ, Shen QH, Xue PY, Zou SH, Wang XJ, Liu X, Wang FM, Yang YP, An XL, Dong ZY, Zhang KP, Zhang XQ, Luo MC, Dvorak J, Tong YP, Wang J, Yang HM, Li ZS, Wang DW, Zhang AM, Wang J (2013) Draft genome of the wheat A-genome progenitor Triticum urartu. Nature 496:87–90
Liu YN, He ZH, Appels R, Xia XC (2012) Functional markers in wheat: current status and future prospects. Theor Appl Genet 125:1–10
Maksimov IV, Cherepanova EA, Kuzmina OI, Yarullina LG, Akhunov AA (2010) Molecular peculiarities of the chitin-binding peroxidases of plants. Russ J Bioorganic Chem 36:293–330
Malosetti M, Ribaut JM, Vargas M, Crossa J, Eeuwijk FA (2008) A multi-trait multi-environment QTL mixed model with an application to drought and nitrogen stress trials in maize (Zea mays L.). Euphytica 161:241–257
Matsuo RR, Dexter J (1980) Relationship between some durum wheat physical characteristics and semolina milling properties. Can J Plant Sci 60:49–53
Morris CF (2002) Puroindolines: the molecular genetic basis of wheat grain hardness. Plant Mol Biol 48:633–647
Passardi F, Longet D, Penel C, Dunand C (2004) The class III peroxidase multigenic family in rice and its evolution in land plants. Phytochemistry 65:1879–1893
Peng JR, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F, Sudhakar D, Christou P, Snape JW, Gale MD, Harberd NP (1999) ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature 400:256–261
Piontek K, Glumoff T, Winterhalter K (1993) Low pH crystal structure of glycosylated lignin peroxidase from Phanerochate chrysosporium at 2.5 Å resolution. FEBS Lett 315:119–124
Poulos TL, Patterson WR, Sundaramoorthy M (1995) The crystal structure of ascorbate and manganese peroxidase: the role of non-haem metal in the catalytic mechanism. Biochem Soc Trans 23:228–232
Ravel C, Praud S, Murigneux A, Canaguier A, Sapet F, Samson D, Balfourier F, Dufour P, Chalhoub B, Brunel D, Beckert M, Charmet G (2006) Single-nucleotide polymorphism frequency in a set of selected lines of bread wheat (Triticum aestivum L.). Genome 49:1131–1139
Revanappa SB, Salimath PV, Prasada Rao UJS (2014) Effect of peroxidase on textural quality dough and arabinoxylan characteristic isolated from whole wheat flour dough. Int J Food Microbiol 17:2131–2141
Rhee SG, Woo HA, Kil IS, Bae SH (2012) Peroxiredoxin functions as a peroxidase and a regulator and sensor of local peroxides. J Biol Chem 287:4403–4410
Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744
Taha SA, Sagi F (1987) Relationships between chemical composition of durum wheat semolina and macaroni quality. II. Ash, carotenoid pigments and oxidative enzymes. Cereal Res Commun 15:123–129
Takasaki S, Kato Y, Murata M, Homma S, Kawakishi S (2005) Effects of peroxidase and hydrogen peroxide on the dityrosine formation and the mixing characteristics of wheat-flour dough. Biosci Biotechnol Biochem 69:1686–1692
Theilade B, Rasmussen SK (1992) Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A. Gene 118:261–266
Trono D, Pastore D, Di Fonzo N (1999) Carotenoid dependent inhibition of durum wheat lipoxygenase. J Cereal Sci 29:99–102
Veldboom LR, Lee M (1996) Genetic mapping of quantitative trait loci in maize in stress and non-stress environments: I. Grain yield and yield components. Crop Sci 36:1310–1319
Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78
Wang SC, Wong D, Forrest K, Allen A, Chao S, Huang BE, Maccaferri M, Salvi S, Milner SG, Cattivelli L, Mastrangelo AM, Whan A, Stephen S, Barker G, Wieseke R, Plieske J, IWGSC, Lillemo M, Mather D, Appels R, Dolferus R, Guedira GB, Korol A, Akhunova AR, Feuillet C, Salse J, Morgante M, Pozniak C, Luo MC, Dvorak J, Morell M, Dubcovsky J, Ganal M, Tuberosa R, Lawley C, Mikoulitch I, Cavanagh C, Edwards KJ, Hayden M, Akhunov E (2014) Characterization of polyploidy wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array. Plant Biotechnol J 12:787–796
Yanagisawa T, Kiribuchi-Otobe C, Hirano H, Suzuki Y, Fujita M (2003) Detection of single nucleotide polymorphism (SNP) controlling the waxy character in wheat by using a derived cleaved amplified polymorphic sequence (dCAPS) marker. Theor Appl Genet 107:84–88
Žilić S, Serpen A, Akillioğlu G, Janković M, Gőkmen V (2012) Distributions of phenolic compounds, yellow pigments and oxidative enzymes in wheat grains and their relation to antioxidant capacity of bran and debranned flour. J Cereal Sci 56:652–658
The authors are grateful to Prof. R. A. McIntosh, Plant Breeding Institute, University of Sydney, for critical review of this manuscript. This study was supported by the National Natural Science Foundation of China (31260327, 31461143021), the National 863 Project (2012AA10A308), the Gene Transformation Projects (2011ZX08009-003, 2011ZX08002004-008), International Science & Technology Cooperation Program of China (2014DFG31690), and China Agriculture Research System (CARS-3-1-3).
Conflict of interest
There are no conflicts of interest for this manuscript.
We declare that these experiments comply with the ethical standards in China, where they were performed.
Communicated by B. Hulke.
Electronic supplementary material
Below is the link to the electronic supplementary material.
About this article
Cite this article
Wei, J., Geng, H., Zhang, Y. et al. Mapping quantitative trait loci for peroxidase activity and developing gene-specific markers for TaPod-A1 on wheat chromosome 3AL. Theor Appl Genet 128, 2067–2076 (2015). https://doi.org/10.1007/s00122-015-2567-0
- Quantitative Trait Locus
- Common Wheat
- Quantitative Trait Locus Analysis
- Chinese Spring
- Recombinant Inbred Line Population