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Resistance to stem rust Ug99 in six bread wheat cultivars maps to chromosome 6DS

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Identified SSR markers ( Xcfd49 and Xbarc183 ) linked with stem rust resistance for efficient use in marker-assisted selection and stacking of resistance genes in wheat breeding programs.

Abstract

More than 80 % of the worldwide wheat (Triticum aestivum L.) area is currently sown with varieties susceptible to the Ug99 race group of stem rust fungus. However, wheat lines Niini, Tinkio, Coni, Pfunye, Blouk, and Ripper have demonstrated Ug99 resistance at the seedling and adult plant stages. We mapped stem rust resistance in populations derived from crosses of a susceptible parent with each of the resistant lines. The segregation of resistance in each population indicated the presence of a single gene. The resistance gene in Niini mapped to short arm of chromosome 6D and was flanked by SSR markers Xcfd49 at distances of 3.9 cM proximal and Xbarc183 8.4 cM distal, respectively. The chromosome location of this resistance was validated in three other populations: PBW343/Coni, PBW343/Tinkio, and Cacuke/Pfunye. Resistance initially postulated to be conferred by the SrTmp gene in Blouk and Ripper was also linked to Xcfd49 and Xbarc183 on 6DS, but it was mapped proximal to Xbarc183 at a similar position to previously mapped genes Sr42 and SrCad. Based on the variation in diagnostic marker alleles, it is possible that Niini and Pfunye may carry different resistance genes/alleles. Further studies are needed to determine the allelic relationships between various genes located on chromosome arm 6DS. Our results provide valuable molecular marker and genetic information for developing Ug99 resistant wheat varieties in diverse germplasm and using these markers to tag the resistance genes in wheat breeding.

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References

  • CIMMYT (2005) Laboratory protocols: CIMMYT applied molecular genetics laboratory, 3rd edn. CIMMYT, Mexico

  • Fetch TG Jr (2007) Virulence of stem rust race TTKS on Canadian wheat cultivars. Can J Plant Pathol 29:441

    Google Scholar 

  • Ghazvini H, Hiebert CW, Zegeye T, Liu S, Dilawari M, Tsilo T, Anderson JA, Rouse MN, Jin Y, Fetch T (2012) Inheritance of resistance to Ug99 stem rust in wheat cultivar Norin 40 and genetic mapping of Sr42. Theor Appl Genet 125:817–824

    Article  CAS  PubMed  Google Scholar 

  • Haley SD, Johnson JJ, Peairs FB, Quick JS, Stromberger JA, Clayshulte SR, Butler JD, Rudolph JB, Seabourn BW, Bai GH, Jin Y, Kolmer J (2007) Registration of ‘Ripper’ wheat. J Plant Regist 1:1–6

    Article  Google Scholar 

  • Haley SD, Johnson JJ, Peairs FB, Quick JS, Stromberger JA, Butler JD, Miller HR, Heaton EE, Rudolph JB, Seabourn BW, Bai GH, Jin Y, Kolmer J, Chen XM (2008) Registration of ‘Bill Brown’ wheat. J Plant Regist 2:218–223

    Article  Google Scholar 

  • Hiebert CW, Fetch TG Jr, Zegeye T (2010) Genetics and mapping of stem rust resistance to Ug99 in the wheat cultivar Webster. Theor Appl Genet 121:65–69

    Article  PubMed  Google Scholar 

  • Hiebert CW, Fetch TG, Zegeye T, Thomas JB, Somers DJ, Humphreys DG, McCallum BD, Cloutier S, Singh D, Knott DR (2011) Genetics and mapping of seedling resistance to Ug99 stem rust in Canadian wheat cultivars “Peace” and “AC Cadillac”. Theor Appli Genet 122:143–149

    Article  Google Scholar 

  • Jin Y, Singh RP (2006) Resistance in US wheat to recent eastern African isolates of Puccinia graminis f. sp. tritici with virulence to resistance gene Sr31. Plant Dis 90:476–480

    Article  CAS  Google Scholar 

  • Jin Y, Singh RP, Ward RW, Wanyera R, Kinyua M, Njau P, Fetch T, Pretorius ZA, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 91:1096–1099

    Article  Google Scholar 

  • Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward R, Fetch TG Jr (2008) Detection of virulence to resistance genes Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 92:923–926

    Article  Google Scholar 

  • Jin Y, Szabo LJ, Rouse MN, Fetch TG Jr, Pretorius ZA, Wanyera R, Njau P (2009) Detection of virulence to resistance gene Sr36 within the TTKS race lineage of Puccinia graminis f. sp. tritici. Plant Dis 93:367–370

    Article  CAS  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugenics 12:172–175

    Article  Google Scholar 

  • Laroche A, Demeke T, Gaudet DA, Puchalski B, Frick M, McKenzie R (2000) Development of a PCR marker for rapid identification of the Bt10 gene for common bunt resistance in wheat. Genome 43:217–223

    CAS  PubMed  Google Scholar 

  • Leonard KJ, Szabo LJS (2005) Stem rust of small grains and grasses caused by Puccinia graminis. Mol Plant Pathol 6:99–111

    Article  PubMed  Google Scholar 

  • Lorieux M (2012) MapDisto: fast and efficient computation of genetic linkage maps. Mol Breed 30:1231–1235

    Article  CAS  Google Scholar 

  • Lowe I, Cantu D, Dubcovsky J (2011) Durable resistance to the wheat rust: integrating systems biology and traditional phenotype-based research methods to guide the deployment of resistance genes. Euphytica 179:69–79

    Article  Google Scholar 

  • Ma H, Kong Z, Fu B, Li N, Zhang L, Jia H, Ma Z (2011) Identification and mapping of a new powdery mildew resistance gene on chromosome 6D of common wheat. Theor Appl Genet 123:1099–1106

    Article  CAS  PubMed  Google Scholar 

  • Mago R, Spielmeyer W, Lawrence GJ, Lagudah ES, Ellis JG, Pryor A (2002) Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat-rye translocation lines. Theor Appl Genet 104:1317–1324

    Article  CAS  PubMed  Google Scholar 

  • Mago R, Miah H, Lawrence GJ, Wellings CR, Spielmeyer W, Bariana HS, McIntosh RA, Pryor AJ, Ellis JG (2005) High-resolution mapping and mutation analysis separate the rust resistance genes Sr31, Lr26 and Yr9 on the short arm of rye chromosome 1. Theor Appl Genet 112:41–50

    Article  CAS  PubMed  Google Scholar 

  • Mago R, Brown-Guedira G, Dreisigacker S, Breen J, Jin Y, Singh R, Appels R, Lagudah ES, Ellis J, Spielmeyer W (2011) An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat. Theor Appl Genet 122:735–744

    Article  CAS  PubMed  Google Scholar 

  • McFadden ES (1930) A successful transfer of emmer characters to vulgare wheat. Agron J 22:1020–1034

    Article  Google Scholar 

  • McIntosh RA (1988) The role of specific genes in breeding for durable stem rust resistance in wheat and triticale. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rusts of wheat. CIMMYT, Mexico, pp 1–9

    Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rust an atlas of resistance genes. CSIRO, Australia

    Google Scholar 

  • McVey DV, Hamilton K (1985) Stem rust gene from Triumph 64 identified in four other winter wheats. Plant Dis 69:217–218

    Article  Google Scholar 

  • Mebrate SA, Oerke EC, Dehne HW, Pillen K (2008) Mapping of the leaf rust resistance gene Lr38 on wheat chromosome arm 6DL using SSR markers. Euphytica 162:457–466

    Article  CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genome regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  CAS  PubMed  Google Scholar 

  • Nagarajan S, Joshi LM (1975) An historical account of wheat rust epidemics in India and their significance. Cereal Rust Bull 2:29–33

    Google Scholar 

  • Nazari K, Mafi M, Yahyaoui A, Singh RP, Park RF (2009) Detection of wheat stem rust (Puccinia graminis f. sp. tritici) race TTKSK in Iran. Plant Dis 93:317

    Article  Google Scholar 

  • Njau P, Jin Y, Huerta-Espino J, Keller B, Singh RP (2010) Identification and evaluation of sources of resistance to stem rust race Ug99 in wheat. Plant Dis 94:413–419

    Article  Google Scholar 

  • Njau PN, Bhavani S, Huerta-Espino J, Keller B, Singh RP (2013) Identification of QTL associated with durable adult plant resistance to stem rust race Ug99 in wheat cultivar ‘Pavon76’. Euphytica 190:33–44

    Article  Google Scholar 

  • Peterson RF, Campbell AB, Hannah AE (1948) A diagrammatic scale for estimating rust intensity of leaves and stem of cereals. Can J Res Sect C26:496–500

    Article  Google Scholar 

  • Pretorius Z, Singh RP, Wagoire WW, Payne TS (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Dis 84:203

    Article  Google Scholar 

  • Pretorius ZA, Bender CM, Visser B, Terefe T (2010) First report of a Puccinia graminis f. sp. tritici race virulent to the Sr24 and Sr31 wheat stem rust resistance genes in South Africa. Plant Dis 94:784

    Article  Google Scholar 

  • Qi LL, Pumphrey MO, Friebe B, Zhang P, Qian C, Bowden RL, Rouse MN, Jin U, Gill BS (2011) A novel Robertsonian translocation event leads to transfer of a stem rust resistance gene (Sr52) effective against race Ug99 from Dasypyrum villosum into bread wheat. Theor Appl Genet 123:159–167

    Article  CAS  PubMed  Google Scholar 

  • Rajaram S, Singh RP, Torres E (1988) Current CIMMYT approaches in breeding wheat for rust resistance. In: Simmonds NW, Rajaram S (eds) Breeding strategies for resistance to the rust of wheat. CIMMYT, Mexico, D.F, pp 101–118

    Google Scholar 

  • Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023

    CAS  PubMed  Google Scholar 

  • Roelfs AP (1978) Estimated losses caused by rust in small grains cereals in the United States—1918. Misc Publ US Dep Agric 1363:1–18

    Google Scholar 

  • Roelfs AP, Singh RP, Saari EE (1992) Rust diseases of wheat: concepts and methods of disease management. CIMMYT, Mexico, p 81

    Google Scholar 

  • Rouse MN, Wanyera R, Njau P, Jin Y (2011) Sources of resistance to stem rust race Ug99 in spring wheat germplasm. Plant Dis 95:762–766

    Article  Google Scholar 

  • Rouse MN, Nava IC, Chao S, Anderson JA, Jin Y (2012) Identification of markers linked to the race Ug99 effective stem rust resistance gene Sr28 in wheat (Triticum aestivum L.). Theor Appl Genet 125:877–885

    Article  PubMed  Google Scholar 

  • Sambasivam PK, Bansal UK, Hayden MJ, Dvorak J, Lagudah ES, Bariana HS (2008) Identification of markers linked with stem rust resistance genes Sr33 and Sr45. In: Appels R et al (eds) Proceedings of the 11th international wheat genetics symposium, vol 2. Sydney University Press, Australia, pp 351–353

    Google Scholar 

  • Sharma RK, Singh PK, Vinod, Joshi AK, Bhardwaj SC, Bains NS, Sukwinder-Singh (2013) Protecting south Asia wheat production from stem rust (Ug99) epidemic. J Phytopathol 161:299–307

  • Simons K, Abate Z, Chao SM, Zhang WJ, Rouse M, Jin Y, Elias E, Dubcovsky J (2011) Genetic mapping of stem rust resistance gene Sr13 in tetraploid wheat (Triticum turgidum ssp durum L.). Theor Appl Genet 122:649–658

    Article  PubMed  Google Scholar 

  • Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Njau P, Wanyera R, Herrera-Foessel SA, Ward RW (2008) Will stem rust destroy the world’s wheat crop? Adv Agron 98:271–309

    Article  CAS  Google Scholar 

  • Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Bhavani S, Njau P, Herrera-Foessel SA, Singh PK, Singh S, Velu G (2011a) The emergence of Ug99 races of the stem rust fungus is a threat to world wheat production. Annu Rev Phytopathol 49:465–481

    Article  CAS  PubMed  Google Scholar 

  • Singh RP, Huerta-Espino J, Bhavani S, Herrera-Foessel SA, Singh D, Singh PK, Velu G, Mason RE, Jin Y, Njau P, Crossa J (2011b) Race non-specific resistance to rust diseases in CIMMYT spring wheats. Euphytica 179:175–186

    Article  Google Scholar 

  • Singh S, Singh RP, Bhavani S, Huerta-Espino J, Lopez-Vera EE (2013) QTL mapping of slow-rusting, adult plant resistance to race Ug99 of stem rust fungus in PBW343/Muu RIL population. Theor Appl Genet 126:1367–1375

    Article  PubMed  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560

    Article  CAS  PubMed  Google Scholar 

  • Stakman EC, Stewart DM, Loegering WQ (1962) Identification of physiologic races of Puccinia graminis var. tritici. U.S. Dep Agric Agric Res Serv E-617

  • Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744

    Article  CAS  Google Scholar 

  • Tsilo TJ, Jin Y, Anderson JA (2008) Diagnostic microsatellite markers for the detection of stem rust resistance gene Sr36 in diverse genetic backgrounds of wheat. Crop Sci 48:253–261

    Article  CAS  Google Scholar 

  • Van Ooijen JW (2006) JoinMap.4.0: Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen

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Acknowledgments

This research work was supported by the Durable Rust Resistance in Wheat (DRRW) project managed by Cornell University with the Bill & Melinda Gates Foundation and the UK Agency for International Development (DFID). E.E. Lopez-Vera appreciates the Ph.D. fellowship from Mexico’s Consejo Nacional de Ciencia y Tecnología (CONACYT). Editing assistance was received from E. Quilligan (CIMMYT).

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The authors declare that there are no conflicts of interest.

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Correspondence to Sukhwinder Singh.

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Communicated by B. Keller.

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Lopez-Vera, E.E., Nelson, S., Singh, R.P. et al. Resistance to stem rust Ug99 in six bread wheat cultivars maps to chromosome 6DS. Theor Appl Genet 127, 231–239 (2014). https://doi.org/10.1007/s00122-013-2212-8

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