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Theoretical and Applied Genetics

, Volume 126, Issue 11, pp 2865–2878 | Cite as

Mapping of QTL conferring resistance to sharp eyespot (Rhizoctonia cerealis) in bread wheat at the adult plant growth stage

  • Jiang Chen
  • Guo Hui Li
  • Zi Yi Du
  • Wei Quan
  • Hu Yan Zhang
  • Ming Zhe Che
  • Zhen Wang
  • Zhong Jun ZhangEmail author
Original Paper

Abstract

Key message

Seven sharp eyespot resistance QTL were detected consistently across five environments and delimited to seven DNA marker intervals, respectively, six of which were independent of plant height and heading time.

Abstract

Sharp eyespot, caused mainly by the soil-borne fungus Rhizoctonia cerealis, is one of the important diseases of bread wheat (Triticum aestivum L.). This disease has escalated into a major threat to wheat production in some regions of the world. Wheat resistance to sharp eyespot can be a potential means to reduce the needs for application of fungicides and agricultural inputs. In the present study, the winter wheat lines, Luke and AQ24788-83, both of which possess quantitative resistance to sharp eyespot, were crossed and a population consisting 241 recombinant-inbred lines (RILs) was constructed. These RILs were assessed for sharp eyespot resistance by conducting five field and greenhouse trials during the period from 2008 to 2012, and they were genotyped with 549 simple-sequence repeat DNA markers. Seven quantitative trait loci (QTL) were detected consistently across the five trial environments to be associated with the sharp eyespot resistance. They were mapped on chromosomes 1A, 2B, 3B, 4A, 5D, 6B, and 7B. Four of these QTL are unequivocally novel, while it is possible that the other three might also be novel. Plant height and heading date of the 241 RILs were recorded in the four field trials. All of the seven disease resistance QTL were independent of plant height and heading time except one that was significantly associated with plant heading time. This association might be attributed genetically to a single QTL, or to different but closely linked QTL. In the case of single QTL, pleiotropism might be involved or the sharp eyespot resistance might be conferred in a physical instead of physiological nature.

Keywords

Quantitative Trait Locus Cetyl Trimethyl Ammonium Bromide Composite Interval Mapping Resistance Quantitative Trait Locus Trial Environment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

This study was supported by the National Natural Science Foundation of China (30871612), the Commonweal Specialized Research Fund of China Agriculture (200903035, nyhyzx3-16), the National Basic Research Program of China (2013CB127700), and the Program for Changjiang Scholars and Innovative Research Team (IRT1042).

Conflict of interest

The authors declare no conflict of interest.

Ethical standards

The experiments comply with the current laws of P. R. China.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Jiang Chen
    • 1
  • Guo Hui Li
    • 2
  • Zi Yi Du
    • 1
  • Wei Quan
    • 1
  • Hu Yan Zhang
    • 1
  • Ming Zhe Che
    • 1
  • Zhen Wang
    • 1
  • Zhong Jun Zhang
    • 1
    Email author
  1. 1.Department of Plant PathologyChina Agricultural UniversityBeijingPeople’s Republic of China
  2. 2.College of ScienceChina Agricultural UniversityBeijingPeople’s Republic of China

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