Theoretical and Applied Genetics

, Volume 126, Issue 11, pp 2825–2834 | Cite as

Genetic mapping of QTL for resistance to Fusarium head blight spread (type 2 resistance) in a Triticum dicoccoides × Triticum durum backcross-derived population

  • Maria Buerstmayr
  • Abdallah Alimari
  • Barbara Steiner
  • Hermann BuerstmayrEmail author
Original Paper


Improvement of resistance to Fusarium head blight (FHB) is a continuous challenge for durum wheat breeders, particularly due to the limited genetic variation within this crop species. We accordingly generated a backcross-derived mapping population using the type 2 FHB resistant Triticum dicoccoides line Mt. Gerizim #36 as donor and the modern Austrian T. durum cultivar Helidur as recipient; 103 BC1F6:7 lines were phenotyped for type 2 FHB resistance using single-spikelet inoculations and genotyped with 421 DNA markers (SSR and AFLP). QTL mapping revealed two highly significant QTL, mapping to chromosomes 3A and 6B, respectively. For both QTL the T. dicoccoides allele improved type 2 FHB resistance. Recombinant lines with both favorable alleles fixed conferred high resistance to FHB similar to that observed in the T. dicoccoides parent. The results appear directly applicable for durum wheat resistance breeding.


Plant Height Amplify Fragment Length Polymorphism Durum Wheat Fusarium Head Blight Amplify Fragment Length Polymorphism Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Special thank for the excellent technical assistance by Lisa Schmid and Matthias Fidesser. We thank Marc Lemmens (IFA-Tulln, Austria) for providing the Fusarium inoculum and Tzion Fahima and Tami Krugman (University of Haifa, Israel) for providing the T. dicoccoides line. Many thanks to Clare Nelson (USA) for his suggestions to improve the manuscript. Abdallah Alimari was supported by a North–South Dialogue grant, funded by the Austrian Ministry of Foreign Affairs and managed by the Austrian Academic Exchange Service (OEAD).

Supplementary material

122_2013_2174_MOESM1_ESM.pdf (11 kb)
Supplementary material 1 (PDF 10 kb)
122_2013_2174_MOESM2_ESM.pdf (9 kb)
Supplementary material 2 (PDF 9 kb)


  1. Agostinelli AM, Clark AJ, Brown-Guedira G, Van Sanford DA (2012) Optimizing phenotypic and genotypic selection for Fusarium head blight resistance in wheat. Euphytica 186:115–126CrossRefGoogle Scholar
  2. Atanasoff D (1920) Fusarium-blight (scab) of wheat and other cereals. J Agri Res 20:1–32Google Scholar
  3. Bai G, Shaner G (1994) Scab of wheat: prospects for control. Plant Dis 78:760–766CrossRefGoogle Scholar
  4. Bai G, Shaner G (1996) Variation in Fusarium graminearum and cultivar resistance to wheat scab. Plant Dis 80:975–979CrossRefGoogle Scholar
  5. Beardall JM, Miller JD (1994) Diseases in humans with mycotoxins as possible causes. In: Miller JD, Trenholm HL (eds) Mycotoxins in grain: compounds other than aflatoxin. Eagan Press, St. Paul, pp 487–539Google Scholar
  6. Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I: resistance to fungal spread (type II resistance). Theor Appl Genet 104:84–91PubMedCrossRefGoogle Scholar
  7. Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M (2003a) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II: resistance to fungal penetration and spread. Theor Appl Genet 107:503–508PubMedCrossRefGoogle Scholar
  8. Buerstmayr H, Stierschneider M, Steiner B, Lemmens M, Griesser M, Nevo E, Fahima T (2003b) Variation for resistance to head blight caused by Fusarium graminearum in wild emmer (Triticum dicoccoides) originating from Israel. Euphytica 130:17–23CrossRefGoogle Scholar
  9. Buerstmayr H, Ban T, Anderson JA (2009) QTL mapping and marker-assisted selection for fusarium head blight resistance in wheat: a review. Plant Breed 128:1–26CrossRefGoogle Scholar
  10. Buerstmayr M, Lemmens M, Steiner B, Buerstmayr H (2011) Advanced backcross QTL mapping of resistance to Fusarium head blight and plant morphological traits in a Triticum macha × T. aestivum population. Theor Appl Genet 123:293–306PubMedCrossRefGoogle Scholar
  11. Buerstmayr M, Huber K, Heckmann J, Steiner B, Nelson JC, Buerstmayr H (2012) Mapping of QTL for Fusarium head blight resistance and morphological and developmental traits in three backcross populations derived from Triticum dicoccum × Triticum durum. Theor Appl Genet 125:1751–1765PubMedCrossRefGoogle Scholar
  12. Chen J, Griffey CA, Maroof MAS, Stromberg EL, Biyashev RM, Zhao W, Chappell MR, Pridgen TH, Dong Y, Zeng Z (2006) Validation of two major quantitative trait loci for Fusarium head blight resistance in Chinese wheat line W14. Plant Breed 125:99–101CrossRefGoogle Scholar
  13. Chen XF, Faris JD, Hu JG, Stack RW, Adhikari T, Elias EM, Kianian SF, Cai XW (2007) Saturation and comparative mapping of a major Fusarium head blight resistance QTL in tetraploid wheat. Mol Breed 19:113–124CrossRefGoogle Scholar
  14. Cuthbert P, Somers D, Brule-Babel A (2007) Mapping of Fhb2 on chromosome 6BS: a gene controlling Fusarium head blight field resistance in bread wheat (Triticum aestivum L.). Theor Appl Genet 114:429–437PubMedCrossRefGoogle Scholar
  15. de Givry S, Bouchez M, Chabrier P, Milan D, Schiex T (2005) CARTHAGENE: multi population integrated genetic and radiated hybrid mapping. Bioinformatics 21:1703–1704PubMedCrossRefGoogle Scholar
  16. de Nijs M, Rombouts F, Notermans S (1996) Fusarium molds and their mycotoxins. J Food Saf 16:15–58CrossRefGoogle Scholar
  17. Dexter JE, Nowicki TW (2003) Safety assurance and quality assurance issues associated with Fusarium head blight in wheat. In: Leonard KJ, Bushnell WR (eds) Fusarium head blight of wheat and barley. APS Press, St. Paul, pp 420–460Google Scholar
  18. Draeger R, Gosman N, Steed A, Chandler E, Thomsett M, Schondelmaier J, Srinivasachary, Buerstmayr H, Lemmens M, Schmolke M, Mesterhazy A, Nicholson P (2007) Identification of QTLs for resistance to Fusarium head blight, DON accumulation and associated traits in the winter wheat variety Arina. Theor Appl Genet 115:617–625PubMedCrossRefGoogle Scholar
  19. Elias EM, Manthey FA, Stack RW, Kianian SF (2005) Breeding efforts to develop Fusarium head blight resistant durum wheat in North Dakota. In: Canty SM, Boring T, Wardwell J, Siler L, Ward RW (eds) Proc 2005 national Fusarium head blight forum. Milwaukee, WI, 11–13 Dec 2005. Michigan State University, East Lansing, pp 25–26Google Scholar
  20. Ellis M, Spielmeyer W, Gale K, Rebetzke G, Richards R (2002) “Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theor Appl Genet 105:1038–1042PubMedCrossRefGoogle Scholar
  21. Gervais L, Dedryver F, Morlais JY, Bodusseau V, Negre S, Bilous M, Groos C, Trottet M (2003) Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat. Theor Appl Genet 106:961–970PubMedGoogle Scholar
  22. Ghavami F, Elias EM, Mamidi S, Ansari O, Sargolzaei M, Adhikari T, Mergoum M, Kianian SF (2011) Mixed model association mapping for Fusarium head blight resistance in Tunisian-derived durum wheat populations. G3 (Bethesda) 1:209–218CrossRefGoogle Scholar
  23. Gladysz C, Lemmens M, Steiner B, Buerstmayr H (2007) Evaluation and genetic mapping of resistance to Fusarium head blight in Triticum dicoccoides. Isr J of Plant Sci 55:263–266CrossRefGoogle Scholar
  24. Haley SC, Knott AS (1992) A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity 69:315–324PubMedCrossRefGoogle Scholar
  25. Handa H, Namiki B, Xu D, Ban T (2008) Dissecting of the FHB resistance QTL on the short arm of wheat chromosome 2D using a comparative genomic approach: from QTL to candidate gene. Mol Breed 27:71–84CrossRefGoogle Scholar
  26. Hartl L, Mohler V, Zeller FJ, Hsam SLK, Schweizer G (1999) Identification of AFLP markers closely linked to the powdery mildew resistance genes Pm1c and Pm4a in common wheat (Triticum aestivum L.). Genome 42:322–329Google Scholar
  27. Hilton A, Jenkinson P, Hollins T, Parry D (1999) Relationship between cultivar height and severity of Fusarium ear blight in wheat. Plant Pathol 48:202–208CrossRefGoogle Scholar
  28. Huhn M, Elias E, Ghavami F, Kianian S, Chao S, Zhong S, Alamri M, Yahyaoui A, Mergoum M (2012) Tetraploid Tunisian wheat germplasm as a new source of Fusarium head blight resistance. Crop Sci 52:136–145CrossRefGoogle Scholar
  29. Klahr A, Zimmermann G, Wenzel G, Mohler V (2007) Effects of environment, disease progress, plant height and heading date on the detection of QTLs for resistance to Fusarium head blight in an European winter wheat cross. Euphytica 154:17–28CrossRefGoogle Scholar
  30. Kumar S, Stack R, Friesen T, Faris J (2007) Identification of a novel Fusarium head blight resistance quantitative trait locus on chromosome 7A in tetraploid wheat. Phytopathology 97:592–597PubMedCrossRefGoogle Scholar
  31. Lin F, Kong ZX, Zhu HL, Xue SL, Wu JZ, Tian DG, Wei JB, Zhang CQ, Ma ZQ (2004) Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 × Wangshuibai population. I: type II resistance. Theor Appl Genet 109:1504–1511PubMedCrossRefGoogle Scholar
  32. Lin F, Xue SL, Zhang ZZ, Zhang CQ, Kong ZX, Yao GQ, Tian DG, Zhu HL, Li CJ, Cao Y, Wei JB, Luo QY, Ma ZQ (2006) Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 × Wangshuibai population. II: type I resistance. Theor Appl Genet 112:528–535PubMedCrossRefGoogle Scholar
  33. Mao SL, Wei YM, Cao W, Lan XJ, Yu M, Chen ZM, Chen GY, Zheng YL (2010) Confirmation of the relationship between plant height and Fusarium head blight resistance in wheat (Triticum aestivum L.) by QTL meta-analysis. Euphytica 174:343–356CrossRefGoogle Scholar
  34. McCartney CA, Somers DJ, Fedak G, DePauw RM, Thomas J, Fox SL, Humphreys DG, Lukow O, Savard ME, McCallum BD, Gilbert J, Cao W (2007) The evaluation of FHB resistance QTLs introgressed into elite Canadian spring wheat germplasm. Mol Breed 20:209–221CrossRefGoogle Scholar
  35. McMullen MP, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:1340–1348CrossRefGoogle Scholar
  36. Mesterhazy A (1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breed 114:377–386CrossRefGoogle Scholar
  37. Miedaner T, Voss H (2008) Effect of dwarfing Rht genes on Fusarium head blight resistance in two sets of near-isogenic lines of wheat and check cultivars. Crop Sci 48:2115–2122CrossRefGoogle Scholar
  38. Nelson J (1997) QGENE: software for marker-based genomic analysis and breeding. Mol Breed 3:239–245CrossRefGoogle Scholar
  39. Nevo E, Korol AB, Beiles A, Fahima T (2002) Evolution of wild emmer and wheat improvement: population genetics, genetic resources, and genome organization of wheat’s progenitor, Triticum dicoccoides. Springer-Verlag, BerlinCrossRefGoogle Scholar
  40. Nyquist WE (1991) Estimation of heritability and prediction of selection response in plant populations. Crit Rev Plant Sci 10:235–322CrossRefGoogle Scholar
  41. Oliver R, Stack R, Miller J, Cai X (2007) Reaction of wild emmer wheat accessions to Fusarium head blight. Crop Sci 47:893–899CrossRefGoogle Scholar
  42. Oliver RE, Cai X, Friesen TL, Halley S, Stack RW, Xu SS (2008) Evaluation of Fusarium head blight resistance in tetraploid wheat (Triticum turgidum L.). Crop Sci 48:213–222CrossRefGoogle Scholar
  43. Otto CD, Kianian SF, Elias EM, Stack RW, Joppa LR (2002) Genetic dissection of a major Fusarium head blight QTL in tetraploid wheat. Plant Mol Biol 48:625–632PubMedCrossRefGoogle Scholar
  44. Paillard S, Schnurbusch T, Tiwari R, Messmer M, Winzeler M, Keller B, Schachermayr G (2004) QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.). Theor Appl Genet 109:323–332PubMedCrossRefGoogle Scholar
  45. Roeder SM, Korzun K, Wendehake K, Plaschke J, Tixier HM, Leroy P, Ganal WM (1998) A microsatellite map of wheat. Genetics 149:2007–2023Google Scholar
  46. Ruan Y, Comeau A, Langevin F, Hucl P, Clarke JM, Brule-Babel A, Pozniak CJ (2012) Identification of novel QTL for resistance to Fusarium head blight in a tetraploid wheat population. Genome 55:853–864PubMedCrossRefGoogle Scholar
  47. Saghai Maroof MAK, Soliman RA, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer length polymorphism in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018PubMedCrossRefGoogle Scholar
  48. SAS Institute Inc (2008) SAS/STAT®9.2 user’s guide. Cary, NCGoogle Scholar
  49. Schmolke M, Zimmermann G, Buerstmayr H, Schweizer G, Miedaner T, Korzun V, Ebmeyer E, Hartl L (2005) Molecular mapping of Fusarium head blight resistance in the winter wheat population dream/lynx. Theor Appl Genet 111:747–756PubMedCrossRefGoogle Scholar
  50. Schmolke M, Zimmermann G, Schweizer G, Miedaner T, Korzun V, Ebmeyer E, Hartl L (2008) Molecular mapping of quantitative trait loci for field resistance to Fusarium head blight in a European winter wheat population. Plant Breed 127:459–464CrossRefGoogle Scholar
  51. Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838Google Scholar
  52. Somers DJ, Fedak G, Savard M (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46:555–564PubMedCrossRefGoogle Scholar
  53. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  54. Somers DJ, Fedak G, Clarke J, Cao WG (2006) Mapping of FHB resistance QTLs in tetraploid wheat. Genome 49:1586–1593PubMedCrossRefGoogle Scholar
  55. Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560PubMedCrossRefGoogle Scholar
  56. Sourdille P, Guyomarc’h H, Baron C, Gandon B, Chiquet V, Artiguenave F, Edwards K, Foisset N, Dufour P (2001) Improvement of the genetic maps of wheat using new microsatellite markers. Plant Animal Genome IX Abstracts, p 167Google Scholar
  57. Srinivasachary, Gosman N, Steed A, Simmonds J, Leverington-Waite M, Wang Y, Snape J, Nicholson P (2008) Susceptibility to Fusarium head blight is associated with the Rht-D1b semi-dwarfing allele in wheat. Theor Appl Genet 116:1145–1153PubMedCrossRefGoogle Scholar
  58. Srinivasachary, Gosman N, Steed A, Hollins T, Bayles R, Jennings P, Nicholson P (2009) Semi-dwarfing Rht-B1 and Rht-D1 loci of wheat differ significantly in their influence on resistance to Fusarium head blight. Theor Appl Genet 118:695–702PubMedCrossRefGoogle Scholar
  59. Stack RW, Faris J (2006) Identification of a Fusarium head blight resistance QTL on chromosome 6B in tetraploid wheat [abstract]. Plant and Animal Genome XIV Conference. Abstract no P285:172Google Scholar
  60. Stack RW, Elias EM, Fetch JM, Miller JD, Joppa LR (2002) Fusarium head blight reaction of Langdon durum - Triticum dicoccoides chromosome substitution lines. Crop Sci 42:637–642CrossRefGoogle Scholar
  61. Steiner B, Lemmens M, Griesser M, Scholz U, Schondelmaier J, Buerstmayr H (2004) Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor Appl Genet 109:215–224PubMedCrossRefGoogle Scholar
  62. Talas F, Longin F, Miedaner T (2011) Sources of resistance to Fusarium head blight within Syrian durum wheat landraces. Plant Breed 130:398–400CrossRefGoogle Scholar
  63. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  64. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Horens M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414PubMedCrossRefGoogle Scholar
  65. Wang YZ, Miller JD (1988) Screening techniques and sources of resistance to Fusarium head blight. In: Klatt AR (ed) Wheat production constraints in tropical environments. CIMMYT, DF, pp 239–250Google Scholar
  66. Xue SL, Li GQ, Jia HY, Xu F, Lin F, Tang MZ, Wang Y, An X, Xu HB, Zhang LX, Kong ZX, Ma ZQ (2010) Fine mapping Fhb4, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 121:147–156PubMedCrossRefGoogle Scholar
  67. Yan W, Li H, Cai S, Ma H, Rebetzke G, Liu C (2011) Effects of plant height on type I and type II resistance to Fusarium head blight in wheat. Plant Pathol 60:506–512CrossRefGoogle Scholar
  68. Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Maria Buerstmayr
    • 1
  • Abdallah Alimari
    • 2
  • Barbara Steiner
    • 1
  • Hermann Buerstmayr
    • 1
    Email author
  1. 1.BOKU-University of Natural Resources and Life Sciences, ViennaDepartment for Agrobiotechnology, TullnTullnAustria
  2. 2.Ministry of AgricultureNational Agricultural Research Center (NARC) JeninWest BankPalestine

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