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Theoretical and Applied Genetics

, Volume 126, Issue 11, pp 2777–2789 | Cite as

Cbf14 copy number variation in the A, B, and D genomes of diploid and polyploid wheat

  • Taniya Dhillon
  • Eric J. StockingerEmail author
Original Paper

Abstract

Freezing tolerance and winter hardiness are complex traits. In the Triticeae, two loci on the group 5 chromosome homoeologs are repeatedly identified as having major effects on these traits. Recently, we found that segments of the genomic region at one of these loci, Frost resistance-2 (Fr-2) is copy number variable in barley. Freezing-tolerant winter-hardy genotypes have greater tandem copy numbers of the genomic region encompassing the C-repeat binding factor genes Cbf2A and Cbf4B at Fr-H2 than the less freezing-tolerant nonwinter-hardy genotypes. Here we report that in wheat the Cbf14 gene at Fr-2 is copy number variable. Using DNA blot hybridizations, we estimated copy numbers of Cbf14 across the different genomes of diploid and polyploid wheat. Copy numbers of Cbf14 are lower in the B genome than in the A and D genomes across all ploidy levels. Among hexaploid red wheats, winter genotypes harbor greater Cbf14 copy numbers than spring genotypes. Cbf14 copy numbers also vary across the red winter wheats such that hard wheats harbor greater copy numbers than soft wheats. Analysis of hexaploid wheat chromosome 5 substitution lines indicates that Cbf14 copy numbers in the introgressions are stable in the different backgrounds. Taken together our data suggest that higher copy number states existed in the diploid wild ancestors prior to the polyploidization events and that the loss of Cbf14 copies occurred in the cultivated germplasm.

Keywords

Hexaploid Wheat Freezing Tolerance Substitution Line Tetraploid Wheat Diploid Wheat 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Drs. Harold E. Bockelman, Bikram S. Gill, David F. Garvin, P. Stephen Baenziger, and Carl A. Griffey for providing wheat accessions and genetic stocks. We thank Alexandra Shaffner for DNA extractions. We also thank the anonymous reviewers for helpful suggestions and Laura J. Chapin for help in making changes to some of the figures during the revision. This work was supported by grants from SEEDS: OARDC Research Enhancement Competitive Grants Program (2009127) and the Ohio Plant Biotechnology Consortium (2010-011). Salaries and research support in the Stockinger laboratory provided by state and federal funds appropriated to The Ohio State University, Ohio Agricultural Research and Development Center.

Supplementary material

122_2013_2171_MOESM1_ESM.docx (28.4 mb)
Supplementary material (DOCX 29113 kb)

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© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Department of Horticulture and Crop ScienceThe Ohio State University/Ohio Agricultural Research and Development Center (OARDC)WoosterUSA

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