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Fine mapping of qhir1 influencing in vivo haploid induction in maize

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Abstract

Production of haploids by the in vivo haploid induction method has now become routine for generating new inbred lines in maize. In previous studies, a major quantitative trait locus (QTL) (qhir1) located in bin 1.04 was detected, explaining up to 66 % of the genotypic variance for haploid induction rate (HIR). Our objectives were to (1) fine-map qhir1 and (2) identify closely linked markers useful for marker-assisted breeding of new inducers. For this purpose, we screened a mapping population of 14,375 F2 plants produced from a cross between haploid inducer UH400 and non-inducer line 1680 to identify recombinants. Based on sequence information from the B73 reference genome, markers polymorphic between the two parents were developed to conduct fine mapping with these recombinants. A progeny test mapping strategy was applied to accurately determine the HIR of the 14 recombinants identified. Furthermore, F3 progeny of recombinant F2 plants were genotyped and in parallel evaluated for HIR. We corroborated earlier studies in that qhir1 has both a significantly positive effect on HIR but also a strong selective disadvantage, as indicated by significant segregation distortion. Altogether, we were able to narrow down the qhir1 locus to a 243 kb region flanked by markers X291 and X263.

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Acknowledgments

This work was supported by grants from the National Projects (CARS-02-09, 2009CB118400, 2011AA10A103, 2012AA10A305) and DFG, Grant No. 1070/1, International Research Training Group “Sustainable Resource Use in North China”. A.E.M. also gratefully acknowledges the financial support received from the Bill & Melinda Gates Foundation for implementation of this work through the project Double Haploid Facility for Strengthening Maize Breeding Programs in Africa “A Double Haploid Facility for Strengthening Maize Breeding Programs in Africa”.

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Authors and Affiliations

  1. National Maize Improvement Center of China, China Agricultural University, 100193, Beijing, China

    X. Dong, X. Xu, J. Miao, L. Li, D. Zhang, C. Liu, X. Tian & S. Chen

  2. Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, 100193, Beijing, China

    S. Chen

  3. Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, 70593, Stuttgart, Germany

    X. Mi & A. E. Melchinger

Authors
  1. X. Dong
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  2. X. Xu
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  3. J. Miao
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  4. L. Li
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  5. D. Zhang
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  6. X. Mi
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  7. C. Liu
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  8. X. Tian
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  9. A. E. Melchinger
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  10. S. Chen
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Corresponding authors

Correspondence to A. E. Melchinger or S. Chen.

Additional information

Communicated by F. Hochholdinger.

X. Dong, X. Xu, A. E. Melchinger and S. Chen contributed equally to this work.

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Dong, X., Xu, X., Miao, J. et al. Fine mapping of qhir1 influencing in vivo haploid induction in maize. Theor Appl Genet 126, 1713–1720 (2013). https://doi.org/10.1007/s00122-013-2086-9

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  • DOI: https://doi.org/10.1007/s00122-013-2086-9

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