Dynamic analysis of QTLs on tiller number in rice (Oryza sativa L.) with single segment substitution lines

Abstract:

Twelve single segment substitution lines (SSSLs) in rice, which contain quantitative trait loci (QTLs) for tiller number detected previously, were used to study dynamic expression of the QTLs in this study. These SSSLs and their recipient, Hua-Jing-Xian 74 (HJX74), were used to produce 78 crossing combinations first, and then these combinations and their parents were grown in two planting seasons with three cropping densities. Tiller number was measured at seven developmental stages. QTL effects including main effects (additive, dominance and epistasis), QTL × season and QTL × density interaction effects were analyzed at each measured stage. The additive, dominant and epistatic effects of the 12 QTLs as well as their interaction effects with the seasons and with the densities all display dynamic changes with the development. Eight QTLs are detected with significant additive effects and/or additive × season and/or additive × density interaction effects at least at one developmental stage, and all QTLs have significant dominant and epistatic effects and/or interaction effects involved in. For most of the QTLs dominant effects are much bigger than additive effects, showing overdominance. Each QTL interacts at least with eight other QTLs. Additive and dominant effects of these QTLs are mostly positive while epistatic effects are negative and minor. Most of the QTLs show significant interactions with planting seasons and cropping densities, but the additive effects of QTLs Tn3-1 and Tn3-2, the dominant effects of QTL Tn7 and Tn8, and the epistatic effects of 14 pairs of QTLs are stable across seasons and the dominant effect of QTL Tn3-3 and the epistatic effects of QTL pairs Tn2-1/Tn6-2, Tn2-1/Tn9 and Tn3-3/Tn6-3 are nearly consistent across cropping densities. This paper is the first report of dynamics on dominances and epistasis of QTLs for tiller number in rice and provides abundant information, which is useful to improve rice tiller number via heterosis and/or QTL pyramiding.

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Acknowledgments

This research was supported by State Key Laboratory of Crop Genetics and Gemplasm Enhancement Open Foundation, Nanjing Agricultural University, China (ZW2010001), Xinjiang Key Laboratory of Water Cycle and Utilization in Arid Land Head Open Foundation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences (XJYS0907-2011-02), and The Japan Breeding Program (International Rice Research Institute, IRRI).

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Correspondence to Guoyou Ye.

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G. Liu and H. Zhu contributed equally to this work.

Communicated by J. Wang.

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Liu, G., Zhu, H., Zhang, G. et al. Dynamic analysis of QTLs on tiller number in rice (Oryza sativa L.) with single segment substitution lines. Theor Appl Genet 125, 143–153 (2012). https://doi.org/10.1007/s00122-012-1822-x

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Keywords

  • Dominant Effect
  • Epistatic Effect
  • Tiller Number
  • Best Linear Unbiased Prediction
  • Significant Additive Effect