Quantitative trait loci for root morphology traits under aluminum stress in common bean (Phaseolus vulgaris L.)

Abstract

Aluminum (Al) toxicity is a major limiting factor of crop production in acid soils, which are found mostly in developing countries of the tropics and sub-tropics. Common bean (Phaseolus vulgaris L.) is particularly sensitive to Al toxicity; and development of genotypes with better root growth in Al-toxic soils is a priority. The objectives of the present study were to physiologically assess root architectural traits in a recombinant inbred line (RIL) population of common bean that contrasts for Al resistance (DOR364 × G19833) and to identify quantitative trait loci (QTL) controlling root growth under two nutrient solutions, one with 20 μM Al concentration and the other without Al, both at pH 4.5. A total of 24 QTL were found through composite interval mapping analysis, 9 for traits under Al treatment, 8 for traits under control treatment, and 7 for relative traits. Root characteristics expressed under Al treatment were found to be under polygenic control, and some QTL were identified at the same location as QTL for tolerance to low phosphorous stress, thus, suggesting cross-links in genetic control of adaptation of common bean to different abiotic stresses.

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Abbreviations

ARD:

Average root diameter

DTZ:

Distal part of the transition zone

EZ:

Elongation zone

LG:

Linkage group

NRT:

Average number of root tips

RDW:

Root dry weight

RIL:

Recombinant inbred line

SRL:

Specific root length

TRE:

Tap root elongation rate

TRL:

Total root length

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Acknowledgments

The authors duly acknowledge the partial financial support to CIAT from BMZ/GTZ, Germany through a restricted core project (No. 05.7860.9-001.00) entitled “Fighting drought and aluminum toxicity: Integrating functional genomics, phenotypic screening and participatory evaluation with women and small-scale farmers to develop stress-resistant common bean and Brachiaria for the tropics”. Our thanks also go to S. Beebe, W. Horst, A. F. Rangel, G. Manrique, G. Machado and M. C. Duque for their advice on germplasm, phenotypic screening and statistical analysis.

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Correspondence to Matthew W. Blair.

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Communicated by F. Muehlbauer.

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López-Marín, H.D., Rao, I.M. & Blair, M.W. Quantitative trait loci for root morphology traits under aluminum stress in common bean (Phaseolus vulgaris L.). Theor Appl Genet 119, 449–458 (2009). https://doi.org/10.1007/s00122-009-1051-0

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Keywords

  • Quantitative Trait Locus
  • Common Bean
  • Total Root Length
  • Transgressive Segregation
  • Specific Root Length