Abstract
Mexican races of maize (Zea mays L.) represent a valuable genetic resource for breeding and genetic surveys. We applied simple sequence repeat (SSR) markers to characterize 25 accessions of races of maize from Mexico. Our objectives were to (1) study the molecular genetic diversity within and among these accessions and (2) examine their relationships as assumed previously on the basis of morphological data. A total of 497 individuals were fingerprinted with 25 SSR markers. We observed a high total number of alleles (7.84 alleles per locus) and total gene diversity (0.61), confirming the broad genetic base of the maize races from Mexico. In addition, the accessions were grouped into distinct racial complexes on the basis of a model-based clustering approach. The principal coordinate analyses of the four Modern Incipient hybrids corroborated the proposed parental races of Chalqueño, Cónico Norteño, Celaya, and Bolita on the basis of the morphological data. Consequently, for some of the accessions, hybridizations provide a clue that can further be used to explain the associations among the Mexican races of maize.
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References
Beadle GW (1939) Teosinte and the origin of maize. J Hered 30:245–247
Bretting PK, Goodman MM (1989) Karyological variation in Mesoamerican races of maize and its systematic significance. Econ Bot 43:107–124
Crossa J, Taba S, Wellhausen EJ (1990) Heterotic patterns among Mexican races of maize. Crop Sci 30:1182–1190
Doebley JF (2004) The genetics of maize evolution. Annu Rev Genet 38:37–59
Doebley JF, Goodman MM, Stuber CW (1985) Isozymic variation in the races of maize from Mexico. Am J Bot 72:629–639
Eyre-Walker A, Gaut RL, Hilton H, Feldman DL, Gaut BS (1998) Investigation of the bottleneck leading to the domestication of maize. Proc Natl Acad Sci USA 95:4441–4446
Goodman MM (1972) Distance analysis in biology. Syst Zool 21:174–186
Goodman MM, Brown WL (1988) Races of corn. In: Sprague GF, Dudley JW (eds) Corn and corn Improvement. 3rd edn. Agron Monogr 18. ASA, CSSA, and SSSA, Madison, WI, pp 39–74
Gower JC (1966) Some distance properties of latent root and vector methods used in multivariate analysis. Biometrika 53:325–338
Hollander M, Wolfe DA (1973) Nonparametric statistical inference. Wiley, New York, pp 139–146
Labate JA, Lamkey KR, Mitchell SH, Kresovich S, Sullivan H, Smith JSC (2003) Molecular and historical aspects of Corn Belt dent diversity. Crop Sci 43:80–91
Legendre P, Legendre L (1998) Numerical ecology. 2nd English edn. Elsevier, Amsterdam
Lynch M, Walsh B (1997) Genetics and analysis of quantitative traits. Sinauer Assoc, Sunderland, MA, p 413
Matsuoka Y, Vigouroux Y, Goodman MM, Sanchez Garcia J, Buckler E, Doebley J (2002) A single domestication for maize shown by multilocus microsatellite genotyping. Proc Natl Acad Sci USA 99:6080–6084
Maurer HP, Melchinger AE, Frisch M (2004) Plabsoft: Software for simulation and data analysis in plant breeding. XVIIth EUCARPIA General Congress 2004, Tulln, Austria, pp 359–362
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Nelson OE (1993) The gametophyte factors of maize. In: Freeling M, Walbot V (eds) The maize handbook. Springer, Berlin Heidelberg New York
Pressoir G, Berthaud J (2004) Population structure and strong divergent selection shape phenotypic diversification in maize landraces. Heredity 92:95–101
Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959
R Development Core Team (2004) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-00-3, URL http://www.R-project.org
Rebourg C, Chastanet M, Gouesnard B, Welcker C, Dubreuil P, Charcosset A (2003) Maize introduction into Europe: the history reviewed in the light of molecular data. Theor Appl Genet 106:895–903
Reif JC, Xia XC, Melchinger AE, Warburton ML, Hoisington DA, Beck D, Bohn M, Frisch M (2004) Genetic diversity determined within and among CIMMYT maize populations of tropical, subtropical, and temperate germplasm by SSR markers. Crop Sci 44:326–334
Reif JC, Hamrit S, Heckenberger M, Schipprack W, Bohn M, Melchinger AE (2005) Genetic structure and diversity of European flint maize populations determined with SSR analyzes of individuals and bulks. Theor Appl Genet 111:906–913
Sanchez GJJ, Goodman MM (1992) Relationships among Mexican races of maize. Econ Bot 46:72–85
Sanchez GJJ, Goodman MM, Stuber CW (2000) Isozymatic and morphological diversity in the races of maize of Mexico. Econ Bot 54:43–59
Vigouroux Y, McMullen M, Hittinger CT, Houchins K, Schulz L, Kresovich S, Matsuoka Y, Doebley J (2002) Identifying genes of agronomic importance in maize by screening microsatellites for evidence of selection during domestication. Proc Natl Acad Sci USA 99:9650–9655
Wellhausen EJ, Roberts LM, Hernandez E (in collaboration with Mangelsdorf PC) (1952) Races of maize in Mexico. The Bussey Institution, Harvard University Press, Cambridge, MA
Wright S (1978) Evolution and genetics of populations, Vol. IV. The University of Chicago Press, Chicago, IL, p 91
Acknowledgments
The molecular marker analyses of this research were supported by funds from the German Federal Ministry for Economic Cooperation and Development (BMZ) Project No. 98.7860.4-001-01. The authors thank Leticia Diaz and Ana Lidia Gomez for their excellent technical assistance. The authors thank three anonymous reviewers for their valuable suggestions.
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Reif, J.C., Warburton, M.L., Xia, X.C. et al. Grouping of accessions of Mexican races of maize revisited with SSR markers. Theor Appl Genet 113, 177–185 (2006). https://doi.org/10.1007/s00122-006-0283-5
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DOI: https://doi.org/10.1007/s00122-006-0283-5