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Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most damaging diseases in common wheat (Triticum aestivum L.). With the objective of identifying and tagging new genes for resistance to stripe rust, F1, F2 and F3 populations from the cross Zhou 8425B/Chinese Spring were inoculated with Chinese PST isolate CYR32 in the greenhouse. A total of 790 SSR primers were used to test the parents and resistant and susceptible bulks. The resulting seven polymorphic markers on chromosome 7BL were used for genotyping F2 and F3 populations. Results indicated that Zhou 8425B carries a single dominant resistance gene, temporarily designated YrZH84, closely linked to SSR markers Xcfa2040-7B and Xbarc32-7B with genetic distances of 1.4 and 4.8 cM, respectively. In a seedling test with 25 PST isolates, the reaction patterns of YrZH84 were different from those of lines carrying Yr2 and Yr6. It was concluded that YrZH84 is probably a new stripe rust resistance gene.

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Acknowledgements

The authors are very grateful to Prof. R.A. McIntosh for reviewing this manuscript. This project was funded by the National 863 program (2003AA207090) and National Natural Science Foundation of China (30220140636 and 30471083).

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Authors and Affiliations

  1. Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Zhongguancun South Street 12, 100081, Beijing, China

    Z. F. Li, Z. H. He, G. Q. Li & X. C. Xia

  2. Zhoukou Academy of Agricultural Sciences, Jianshe East Road, 466001, Zhoukou, Henan Province, China

    T. C. Zheng, X. P. Li & G. Y. Yang

  3. International Maize and Wheat Improvement Center (CIMMYT), CIMMYT China Office, c/o CAAS, Zhongguancun South Street 12, 100081, Beijing, China

    Z. H. He

  4. Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Yuanmingyuan West Road 2, 100094, Beijing, China

    S. C. Xu

  5. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico D.F, Mexico

    R. P. Singh

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  1. Z. F. Li
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  2. T. C. Zheng
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  3. Z. H. He
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  9. X. C. Xia
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Correspondence to Z. H. He or X. C. Xia.

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Communicated by P. Langridge

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Li, Z.F., Zheng, T.C., He, Z.H. et al. Molecular tagging of stripe rust resistance gene YrZH84 in Chinese wheat line Zhou 8425B. Theor Appl Genet 112, 1098–1103 (2006). https://doi.org/10.1007/s00122-006-0211-8

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  • DOI: https://doi.org/10.1007/s00122-006-0211-8

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