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Targeting xa13, a recessive gene for bacterial blight resistance in rice

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Abstract

Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most serious diseases of rice worldwide. Thirty bacterial blight resistance (R) genes (21 dominant genes and 9 recessive genes) in rice have been identified. They are the main sources for the genetic improvement of rice for resistance to Xoo. However, little is known about the recessive R genes. To clone and characterize the recessive R genes, we fine-mapped xa13, a fully recessive gene for Xoo resistance, to a DNA fragment of 14.8 kb using the map-based cloning strategy and a series of sequence-based molecular markers. Sequence analysis of this fragment indicated that this region contains only two apparently intact candidate genes (an extensin-like gene and a homologue of nodulin MtN3) and the 5′ end of a predicted hypothetical gene. These results will greatly facilitate the isolation and characterization of xa13. Four PCR-based markers, E6a, SR6, ST9 and SR11 that were tightly linked to the xa13 locus, were also developed. These markers will be useful tools for the marker-assisted selection of xa13 in breeding programs.

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Acknowledgements

This research was supported by grants from the National Program of Research and Development of Transgenic Plants of China, National Special Key Program on Rice Functional Genomics, and the National Natural Science Foundation of China.

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Author notes

  1. Binying Fu & Zhikang Li

    Present address: Chinese Academy of Agricultural Sciences, Institute of Crop Sciences, 100081, Beijing, China

Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070, Wuhan, China

    Zhaohui Chu, Hong Yang, Caiguo Xu, Qifa Zhang & Shiping Wang

  2. Plant Breeding, Genetics and Biotechnology Division, International Rice Research Institute, P. O. Box 933, 1099, Manila, The Philippines

    Binying Fu, Zhikang Li & A. Sanchez

  3. Department of Genetics, University of Georgia, Athens, GA, 30602, USA

    Y. J. Park & J. L. Bennetzen

Authors
  1. Zhaohui Chu
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  2. Binying Fu
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  3. Hong Yang
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  4. Caiguo Xu
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  5. Zhikang Li
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  6. A. Sanchez
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  7. Y. J. Park
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  8. J. L. Bennetzen
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  9. Qifa Zhang
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  10. Shiping Wang
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Corresponding author

Correspondence to Shiping Wang.

Additional information

Communicated by D. J. Mackill

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Chu, Z., Fu, B., Yang, H. et al. Targeting xa13, a recessive gene for bacterial blight resistance in rice. Theor Appl Genet 112, 455–461 (2006). https://doi.org/10.1007/s00122-005-0145-6

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  • DOI: https://doi.org/10.1007/s00122-005-0145-6

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