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Phosphorus deficiency-induced root elongation and its QTL in rice (Oryza sativa L.)

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Abstract

A significant level of root elongation was induced in rice (Oryza sativa) grown under phosphorus-deficient conditions. The root elongation clearly varied among a total of 62 varieties screened under two different phosphorus levels. Two contrasting varieties, ‘Gimbozu’, with a low elongating response and ‘Kasalath’, with a high elongating response, were chosen and crossed to produce a hybrid population for QTL analyses. QTLs for the phosphorus deficiency-induced root elongation were detected by two linkage maps, i.e., one with 82 F3 families constructed by 97 simple sequence repeat (SSR) and sequence-tag site markers and another with 97 F8 lines by 790 amplified fragment length polymorphism and SSR markers. A single QTL for the elongation response was detected on chromosome 6, with a LOD score of 4.5 in both maps and explained about 20% of total phenotypic variance. In addition, this QTL itself, or a region tightly linked with it, partly explained an ability to reduce accumulation of excess iron in the shoots. The identified QTL will be useful to improve rice varieties against a complex nutritional disorder caused by phosphorus deficiency and iron toxicity.

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Acknowledgements

This research was funded by the Japan-IRRI Shuttle Research Project.

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Authors and Affiliations

  1. Laboratory of Plant Genetics And Breeding, College of Bioresource Sciences, Nihon University, Kameino Fujisawa, Kanagawa, 252-8501, Japan

    Akifumi Shimizu & Hiroshi Ikehashi

  2. International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines

    Seiji Yanagihara

  3. Plant Physiology Department, National Institute of Agrobiological Sciences, Kannon-dai Tsukuba, Ibaraki, 305-8602, Japan

    Shinji Kawasaki

Authors
  1. Akifumi Shimizu
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  2. Seiji Yanagihara
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  3. Shinji Kawasaki
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  4. Hiroshi Ikehashi
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Corresponding author

Correspondence to Hiroshi Ikehashi.

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Communicated by Q. Zhang

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Shimizu, A., Yanagihara, S., Kawasaki, S. et al. Phosphorus deficiency-induced root elongation and its QTL in rice (Oryza sativa L.). Theor Appl Genet 109, 1361–1368 (2004). https://doi.org/10.1007/s00122-004-1751-4

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  • DOI: https://doi.org/10.1007/s00122-004-1751-4

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