Abstract
The I-3 gene from the wild tomato species Lycopersicon pennellii confers resistance to race 3 of the devastating vascular wilt pathogen Fusarium oxysporum f. sp. lycopersici. As an initial step in a positional cloning strategy for the isolation of I-3, we converted restriction fragment length polymorphism and conserved orthologue set markers, known genes and a resistance gene analogue (RGA) mapping to the I-3 region into PCR-based sequence characterised amplified region (SCAR) and cleaved amplified polymorphic sequence (CAPS) markers. Additional PCR-based markers in the I-3 region were generated using the randomly amplified DNA fingerprinting (RAF) technique. SCAR, CAPS and RAF markers were used for high-resolution mapping around the I-3 locus. The I-3 gene was localised to a 0.3-cM region containing a RAF marker, eO6, and an RGA, RGA332. RGA332 was cloned and found to correspond to a putative pseudogene with at least two loss-of-function mutations. The predicted pseudogene belongs to the Toll interleukin-1 receptor-nucleotide-binding site-leucine-rich-repeat sub-class of plant disease resistance genes. Despite the presence of two RGA332 homologues in L. esculentum, DNA gel blot and PCR analysis suggests that no other homologues are present in lines carrying I-3 that could be alternative candidates for the gene.
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Acknowledgements
This research was conducted within the Cooperative Research Centre for Tropical Plant Protection, established and supported by the Australian Government’s Cooperative Research Centres Program. The authors would like to thank Dr. Brett Baillie and Dr. Stephen Garland for their involvement in this work, Dr. S. Tanksley for generous provision of the TG clones and Dr. D. Zamir and Dr. J. Scott for generous provision of the IL lines and Fla. introgression lines.
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Hemming, M.N., Basuki, S., McGrath, D.J. et al. Fine mapping of the tomato I-3 gene for fusarium wilt resistance and elimination of a co-segregating resistance gene analogue as a candidate for I-3 . Theor Appl Genet 109, 409–418 (2004). https://doi.org/10.1007/s00122-004-1646-4
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DOI: https://doi.org/10.1007/s00122-004-1646-4