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Sectional relationships in the genus Musa L. inferred from the PCR-RFLP of organelle DNA sequences

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Abstract.

The objective of this study was to construct a molecular phylogeny of the genus Musa using restriction-site polymorphisms of the chloroplast (cpDNA) and mitochondrial DNA (mtDNA). Six cpDNA and two mtDNA sequences were amplified individually in polymerase chain reaction (PCR) experiments in 13 species representing the four sections of Musa. Ensete ventricosum (W.) Ch. was used as the outgroup. The amplified products were digested with ten restriction endonucleases. A total of 79 restriction-site changes were scored in the sample. Wagner parsimony using the branch and bound option defined two lines of evolution in Musa. One lineage comprised species of the sections Australimusa and Callimusa which have a basic number of x = 10 chromosomes, while most species of sections Eumusa and Rhodochlamys (x = 11) formed the other lineage. Musa laterita Cheesman (Rhodochlamys) had identical organellar genome patterns as some subspecies of the Musa acuminata Colla complex. The progenitors of the cultivated bananas, M. acuminata and Musa balbisiana Colla, were evolutionarily distinct from each other. Musa balbisiana occupied a basal position in the cladogram indicating an evolutionarily primitive status. The close phylogenetic relationship between M. laterita and M. acuminata suggests that species of the section Rhodochlamys may constitute a secondary genepool for the improvement of cultivated bananas.

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Acknowledgements.

Funding for this research was provided, in part, by the Directorate General for International Cooperation (DGIC), Belgium. D.C. Nwakanma was funded by the IITA Graduate Research Fellowship Program. This is IITA manuscript number 03/003/JA.

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Author notes

  1. D. C. Nwakanma

    Present address: Medical Research Council (MRC) Laboratories, Fajara, P. O. Box 273, Banjul, Gambia,

  2. M. Pillay

    Present address: IITA-Eastern and Southern Africa Regional Center, P. O. Box 7878, Kampala, Uganda,

  3. A. Tenkouano

    Present address: IITA-Humid Forest Ecoregional Center, BP 2008 Messa, Yaoundé, Cameroon,

Authors and Affiliations

  1. International Institute of Tropical Agriculture, High Rainfall Station, P.M.B. 008, Nchia-Eleme (Port-Harcourt), Rivers State, Nigeria

    D. C. Nwakanma, M. Pillay & A. Tenkouano

  2. Department of Botany and Biotechnology, University of Port Harcourt, Port Harcourt, Nigeria

    B. E. Okoli

  3. IITA, C/o Lambourns UK Ltd., Carolyn House, 26 Dingwall Road, Croydon CR9 3EE, UK

    M. Pillay

Authors
  1. D. C. Nwakanma
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  2. M. Pillay
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  3. B. E. Okoli
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  4. A. Tenkouano
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Correspondence to M. Pillay.

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Communicated by H.F. Linskens

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Nwakanma, D.C., Pillay, M., Okoli, B.E. et al. Sectional relationships in the genus Musa L. inferred from the PCR-RFLP of organelle DNA sequences. Theor Appl Genet 107, 850–856 (2003). https://doi.org/10.1007/s00122-003-1340-y

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  • DOI: https://doi.org/10.1007/s00122-003-1340-y

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