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Colleters in Rubiaceae from forest and savanna: the link between secretion and environment

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Abstract

This study aims to investigate colleters’ secretory function, on cellular level, in Rubiaceae species from contrasting environments looking to explore the association between secretion and environment. We collected samples from eight species of Rubiaceae growing in forest and savanna having standard-type colleters with diverse histochemistry (hydrophilic, lipophilic and mixed secretions) and processed for both conventional and cytochemical study under transmission electron microscopy (TEM). The standard colleters, although similar in morphology and anatomy, exhibited marked differences on cellular level, especially in the abundance and topology of Golgi bodies, endoplasmic reticulum and plastids when comparing forest and savanna species. These differences were clearly aligned with the chemical nature of the secretions they produce, with predominance of hydrophilic secretions in forest species and lipophilic or mixed secretions in savanna species. The combination of methods in electron microscopy revealed the sites of synthesis and intracellular compartmentation of substances, the mechanisms of their secretion from the protoplast and confirmed the involvement of the outer walls of the epithelial cells in the elimination of exudates to the gland surface. Our study suggests a potential environment-associated plasticity of the secretory cells of standard-type colleters in modulating their secretory function performance.

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Acknowledgements

This article is part of the doctoral thesis of F. Tresmondi. We acknowledge the financial support of the National Council for Scientific and Technological Development (CNPq—financial support Proc. 473289/2010 and grants to the SR Machado Proc. 302657/2011-8) and the São Paulo Council for Research (FAPESP—financial support Thematic Project Proc. 2008/55434-7 and grants to the first author—Proc. 2011/02488-5). Thanks to the Electron Microscopy Centre (CME) IBB, UNESP and its technicians for assistance with sample preparations.

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Authors and Affiliations

  1. Graduation Program in Biological Sciences (Botany), Institute of Biosciences (UNESP), São Paulo State University, Rua Prof. Dr. Antonio Celso Wagner Zanin, s/no, Caixa Postal 510, Botucatu, SP, 18618-000, Brazil

    Fernanda Tresmondi

  2. Department of Botany, Institute of Biosciences (UNESP), São Paulo State University, Rua Prof. Dr. Antonio Celso Wagner Zanin, s/no, Caixa Postal 510, Botucatu, SP, 18618-970, Brazil

    Yve Canaveze, Elza Guimarães & Silvia Rodrigues Machado

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  1. Fernanda Tresmondi
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  2. Yve Canaveze
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  3. Elza Guimarães
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  4. Silvia Rodrigues Machado
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Correspondence to Silvia Rodrigues Machado.

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Communicated by: Sven Thatje

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Tresmondi, F., Canaveze, Y., Guimarães, E. et al. Colleters in Rubiaceae from forest and savanna: the link between secretion and environment. Sci Nat 104, 17 (2017). https://doi.org/10.1007/s00114-017-1444-x

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  • DOI: https://doi.org/10.1007/s00114-017-1444-x

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