Fig. 1 | Journal of Molecular Medicine

Fig. 1

From: Glucocorticoids dexamethasone and prednisolone suppress fibroblast growth factor 23 (FGF23)

Fig. 1

Effect of glucocorticoids on Fgf23 expression and protein synthesis in UMR106 and MC3T3-E1 cells. Arithmetic means ± SEM (n = 6, arbitrary units (a. u.)) of relative Fgf23 mRNA expression normalized to Tbp transcript levels in UMR106 cells incubated without (white bars) or with (black bars) dexamethasone (DEXA, a) or prednisolone (b) at the indicated concentrations in the presence of calcitriol (10 nM) for 24 h. *p < 0.05, **p < 0.01, ***p < 0.001 indicate significant differences compared to UMR106 cells treated with 10 nM calcitriol only. (c) Arithmetic means ± SEM of C-terminal FGF23 protein concentration in the cell culture supernatant of UMR106 cells treated with vehicle (white bars) or with 30 nM (n = 8) or 100 nM (n = 6) dexamethasone (DEXA, black bars) in the presence of calcitriol (10 nM) for 48 h. *p < 0.05, **p < 0.01, indicate significant difference compared to UMR106 cells treated with 10 nM calcitriol only. Arithmetic means ± SEM (n = 5, arbitrary units (a. u.)) of relative Fgf23 mRNA expression normalized to Tbp transcript levels in MC3T3-E1 cells at day 7 of osteoblast differentiation incubated with vehicle (white bars) or 30 nM (d) or 100 nM (e) dexamethasone (DEXA, black bars) in the presence of calcitriol (10 nM) for 24 h. **p < 0.01 indicates significant difference compared to control cells. (f) Arithmetic means ± SEM (n = 4, arbitrary units (a. u.)) of relative Fgf23 mRNA expression normalized to Tbp transcript levels in MC3T3-E1 cells at day 14 in osteogenic differentiation medium containing calcitriol (10 nM) and incubated with 100 nM dexamethasone (DEXA, black bar) or vehicle (white bar) for 24 h. **p < 0.01 indicates significant difference compared to vehicle-treated MC3T3-E1 cells. (a and b: one-way ANOVA followed by Dunnett’s multiple comparisons test; c–f: paired t test)

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