European Journal of Wood and Wood Products

, Volume 76, Issue 6, pp 1725–1735 | Cite as

Effect of Punica granatum peel and Melia azedarach bark extracts on durability of European beech and maritime pine

  • Lilia Lajnef
  • Isaura CaceresEmail author
  • Pierre Trinsoutrot
  • Fatima Charrier-El Bouhtoury
  • Naceur Ayed
  • Bertrand Charrier


A method to improve wood durability using natural extracts was evaluated. Wood deterioration is a condition caused by several abiotic and biotic factors including fungal contamination. To date, approaches aiming at the reduction of these contaminants mainly involve the use of chemicals agents. Natural products could represent an alternative strategy. Aqueous extracts of Punica granatum L. (pomegranate) peel and Melia azedarach L. barks were evaluated as antifungal agents to improve natural durability of beech wood and maritime pine. To evaluate the effect of treatments under simulated accelerated ageing of wood by natural conditions, impregnation and leaching tests were performed. Results demonstrated that samples impregnated with pomegranate or M. azedarach solutions notably increased the biological resistance of wood in a dose-dependent manner. These results were confirmed by the reduction in weight losses in treated samples even after 6 weeks of fungal exposure. Moreover, after leaching tests, 20 and 7% (w/v) of pomegranate and M. azedarach extract solutions were demonstrated as the better concentrations to enhance wood durability. Total phenol content and characterization of the phenolic compounds in both, natural extracts and wood samples were analyzed by Folin–Ciocalteu assay and HPLC-DAD. In conclusion, it was demonstrated that the present method can be considered as an effective treatment to increase wood durability while it proposes the valorization of natural extractives in wood industry.



We gratefully acknowledge the Laboratory of Biology FCBA of Technological Institute (Bordeaux, France) as well as local Sawmills (Mont de Marsan, France) for the supplied biological and wood material used in this study.


This project was realized with the financial support of the “Projet Utique CMCU-2012”. Funded by ANR-10-EQPX-16 XYLOFOREST.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Akacha M, Chaieb I, Laarif A, Haouala R, Boughanmi N (2017) Effects of Melia azedarach leaf extracts on nutritional behavior and growth of Spodoptera littoralis. Tunis J Plant Prot 12:61–70Google Scholar
  2. Akhtar Y, Yeoung Y-R, Isman MB (2007) Comparative bioactivity of selected extracts from Meliaceae and some commercial botanical insecticides against two noctuid caterpillars, Trichoplusia in and Pseudaletia unipuncta. Phytochem Rev 7:77–88CrossRefGoogle Scholar
  3. Aloui F, Ayadi N, Charrier F, Charrier B (2004) Durability of European oak (Quercus petraea and Quercus robur) against white rot fungi (Coriolus versicolor): relations with phenol extractives. Holz Roh Werkst 62:286–290CrossRefGoogle Scholar
  4. Al-Zoreky NS (2009) Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. Int J Food Microbiol 134:244–248CrossRefGoogle Scholar
  5. Andrei GM, Lampuri JS, Coto C, de Torres RA (1986) An antiviral factor from Melia azedarach L. prevents Tacaribe virus encephalitis in mice. Experientia 42:843–845CrossRefGoogle Scholar
  6. Ayed N (2011) La pomme de Carthage (Grenade de Tunisie). Le Maghreb Magazine, Tunisie 2:98–100Google Scholar
  7. Brand MA, Anzaldo J, Moreschi JC (2006) Novos produtos para o tratamento preservante da madeira: perspectivas da pesquisa e utilizaçao (new products for the preservation of wood: perspectives of research use). Floresta 36:129–138CrossRefGoogle Scholar
  8. Brimner TA, Boland GJ (2003) A review of the non-target effects of fungi used to biologically control plant diseases. Agric Ecosyst Environ 100:3–16CrossRefGoogle Scholar
  9. Brocco VF, Paes JB, Costa LG, Brazolin S, Donaria M (2017) Potential of teak heartwood extracts as a natural wood preservative. J Clean Prod 142:2093–2099CrossRefGoogle Scholar
  10. Carpinella MC, Ferrayoli CG, Palacios SM (2005) antifungal synergistic effect of scopoletin, a hydroxycoumarin isolated from Melia azedarach L. fruits. J Agric Food Chem 53:2922–2927CrossRefGoogle Scholar
  11. CEN/TS 15083-1 (2005) Durability of wood and wood-based products—determination of the natural durability of solid wood against wood-destroying fungi, test methods—Part 1: basidiomycetes. CEN European Committee for StandardizationGoogle Scholar
  12. Charrier B, Marques M, Haluk JP (1992) HPLC analysis of gallic and ellagic acids in European oakwood (Quercus robur L.) and eucalyptus (Eucalyptus globulus). Holzforschung 46:87–89CrossRefGoogle Scholar
  13. Charrier B, Haluk JP, Metche M (1995) Characterization of European oakwood constituents acting in the brown discolouration during Kiln Drying. Holzforschung 49:168–172CrossRefGoogle Scholar
  14. EN 350-2 (1994) Durability of wood and wood based products—Natural durability of solid wood—Part 2: Guide to natural durability and treatability of selected wood species of importance in Europe. European Committee for StandardizationGoogle Scholar
  15. EN 84 (1997) Wood preservatives—accelerated ageing of treated wood prior to biological testing—leaching procedure. European Committee for StandardizationGoogle Scholar
  16. Endo EH, Garcia Cortez DA, Ueda-Nakamura T, Nakamura CV, Dias Filho BP (2010) Potent antifungal activity of extracts and pure compound isolated from pomegranate peels and synergism with fluconazole against Candida albicans. Res Microbiol 161:534–540CrossRefGoogle Scholar
  17. Fischer UA, Carle R, Kammerer DR (2011) Identification and quantification of phenolic compounds from pomegranate (Punica granatum L.) peel, mesocarp, aril and differently produced juices by HPLC-DAD-ESI/MSn. Food Chem 127:807–821CrossRefGoogle Scholar
  18. Gérardin P (2016) New alternatives for wood preservation based on thermal and chemical modification of wood—a review. Ann For Sci 73:559–570CrossRefGoogle Scholar
  19. Gonzalez-Laredo RF, Rosales-Castro M, Rocha-Guzman NE et al (2015) Wood preservation using natural products. Madera y Bosques 21:63–76CrossRefGoogle Scholar
  20. Guilley E, Charpentier JP, Ayadi N, Snakkers G, Nepveu G, Charrier B (2004) Decay resistance against Coriolus versicolor in Sessile oak (Quercus petraea Liebl.): analysis of the between-tree variability and correlations with extractives, tree growth and other basic wood properties. Wood Sci Technol 38:539–554CrossRefGoogle Scholar
  21. Gullon B, Pintado ME, Pérez-Álvarez JA, Viuda-Martos M (2016) Assessment of polyphenolic profile and antibacterial activity of pomegranate peel (Punica granatum) flour obtained from co-product of juice extraction. Food Control 59:94–98CrossRefGoogle Scholar
  22. Guo G, Wang H, Pomegranin Ng T (2009) An antifungal peptide from pomegranate peels. Protein Pept Lett 16:82–85CrossRefGoogle Scholar
  23. Hedenstrom E, Fagerlund Edfeldt A, Edman M, Jonsson B-G (2016) Resveratrol, piceatannol, and isorhapontigenin from Norway spruce (Picea abies) debarking wastewater as inhibitors on the growth of nine species of wood-decaying fungi. Wood Sci Technol 50:617–629CrossRefGoogle Scholar
  24. Hirasawa M, Takada K (2004) Multiple effects of green tea catechin on the antifungal activity of antimycotics against Candida albicans. J Antimicrob Chemother 53:225–229CrossRefGoogle Scholar
  25. Hofmann T, Nebehaj E, Stefanovits-Bányai É, Albert L (2015) Antioxidant capacity and total phenol content of beech (Fagus sylvatica L.) bark extracts. Ind Crops Prod 77:375–381CrossRefGoogle Scholar
  26. Hu J, Chang S, Peng K, Hu K et al (2015) Bio-susceptibility of shells of Camellia oleifera Abel. fruits to fungi and termites. Int Biodeterior Biodegrad 104:219–223CrossRefGoogle Scholar
  27. Iravani S, Zolfaghari B (2011) Pharmaceutical and nutraceutical effects of Pinus pinaster bark extract. Res Pharm Sci 6:1–11PubMedPubMedCentralGoogle Scholar
  28. Jensen KA Jr, Houtman CJ, Ryan ZC, Hammel KE (2001) Pathways for extracellular fenton chemistry in the brown rot basidiomycete Gloeophyllum trabeum. Appl Environ Microbiol 67:2705–2711CrossRefGoogle Scholar
  29. Kevers C, Falkowski M, Tabart J, Defraigne JO, Dommes J, Pincemail J (2007) Evolution of antioxidant capacity during storage of selected fruits and vegetables. J Agric Food Chem 55:8596–8603CrossRefGoogle Scholar
  30. Kirker GT, Bishell AB, Lebow PK (2016) Laboratory evaluations of durability of southern pine pressure treated with extractives from durable wood species. J Econ Entomol 109:259–266CrossRefGoogle Scholar
  31. Kumar SV, Sanghai DB, Rao CM, Shreedhara CS (2012) Histological and physiochemical standardization of Melia azedarach Linn bark Asian Pacific. J Trop Biomed 2:S284–S289CrossRefGoogle Scholar
  32. Kumar A, Ryparová P, Škapin AS et al (2016) Influence of surface modification of wood with octadecyltrichlorosilane on its dimensional stability and resistance against Coniophora puteana and molds. Cellulose 23:3249–3263CrossRefGoogle Scholar
  33. Künniger T, Gerecke AC, Ulrich A et al (2014) Release and environmental impact of silver nanoparticles and conventional organic biocides from coated wooden façades. Environ Pollut 184:464–471CrossRefGoogle Scholar
  34. Kuppusamy S, Thavamani P, Megharaj M, Nirola R, Lee YB, Naidu R (2016) Assessment of antioxidant activity, minerals, phenols and flavonoid contents of common plant/tree waste extracts. Ind Crops Prod 83:630–634CrossRefGoogle Scholar
  35. Leonowicz A, Matuszewska A, Luterek J et al (1999) Review: Biodegradation of lignin by white rot fungi. Fungal Genet Biol 27:175–185CrossRefGoogle Scholar
  36. Li Z-J, Guo X, Dawuti G, Aibai S (2015) Antifungal activity of ellagic acid in vitro and in vivo. Phytother Res 29:1019–1025CrossRefGoogle Scholar
  37. Lucci P, Pacetti D, Loizzo MR, Frega NG (2015) Punica granatum cv Dente di Cavallo seed ethanolic extract: antioxidant and antiproliferative activities. Food Chem 167:475–483CrossRefGoogle Scholar
  38. Mansour MMA, Salem MZM (2015) Evaluation of wood treated with some natural extracts and Paraloid B-72 against the fungus Trichoderma harzianum: Wood elemental composition, in-vitro and application evidence. Int Biodeterior Biodegrad 100:62–69CrossRefGoogle Scholar
  39. Mena P, Calani L, Dall’Asta C et al (2012) Rapid and comprehensive evaluation of (Poly) phenolic compounds in pomegranate (Punica granatum L.) Juice by UHPLC-MSn. Molecules 17:14821–14840CrossRefGoogle Scholar
  40. Monrroy M, Ortega I, Ramírez M, Baeza J, Freer J (2011) Structural change in wood by brown rot fungi and effect on enzymatic hydrolysis. Enzyme Microb Technol 49:472–477CrossRefGoogle Scholar
  41. Mounguengui S, Saha Tchinda JB, Ndikontar MK et al (2016) Total phenolic and lignin contents, phytochemical screening, antioxidant and fungal inhibition properties of the heartwood extractives of ten Congo Basin tree species. Ann For Sci 73:287–296CrossRefGoogle Scholar
  42. Nasr CB, Ayed N, Metche M (1996) Quantitative determination of the polyphenolic content of pomegranate peel Z Lebensm. Unters Forsch 203:374–378CrossRefGoogle Scholar
  43. Ntalli NG, Vargiu S, Menkissoglu-Spiroudi U, Caboni P (2010) Nematicidal carboxylic acids and aldehydes from Melia azedarach fruits. J Agric Food Chem 58:11390–11394CrossRefGoogle Scholar
  44. Panov D, Terziev N (2009) Study on some alkoxysilanes used for hydrophobation and protection of wood against decay. Int Biodeterior Biodegrad 63:456–461CrossRefGoogle Scholar
  45. Rachokarn S, Piyasaengthong N, Bullangpoti V (2008) Impact of botanical extracts derived from leaf extracts Melia azedarach L. (Meliaceae) and Amaranthus viridis L. (Amaranthaceae) on populations of Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) and detoxification enzyme activities. Commun Agric Appl Biol Sci 73:451–457PubMedGoogle Scholar
  46. Rättö M, Ritschkoff A-C, Viikari L (2004) Enzymatically polymerized phenolic compounds as wood preservatives. Holzforschung 58:440–445CrossRefGoogle Scholar
  47. Rodrigues AMS, Stien D et al (2012) The wood preservative potential of long-lasting Amazonian wood extracts. Int Biodeterior Biodegrad 75:146–149CrossRefGoogle Scholar
  48. Romero N, Saavedra J, Tapia F, Sepulveda B, Aparicio R (2016) Influence of agroclimatic parameters on phenolic and volatile compounds of Chilean virgin olive oils and characterization based on geographical origin, cultivar and ripening stage: Effect of agroclimatic parameters on compounds responsible for the flavor of EVOO. J Sci Food Agric 96:583–592CrossRefGoogle Scholar
  49. Rowell RM (2006) Chemical modification of wood: a short review. Wood Mater Sci Eng 1:29–33CrossRefGoogle Scholar
  50. Saad H, Charrier-El Bouhtoury F, Pizzi A, Rode K, Charrier B, Ayed N (2012) Characterization of pomegranate peels tannin extractives. Ind Crops Prod 40:239–246CrossRefGoogle Scholar
  51. Salem MZM, Zidan YE, El Hadidi NMN, Mansour MMA, Abo Elgat WAA (2016) Evaluation of usage three natural extracts applied to three commercial wood species against five common molds. Int Biodeterior Biodegrad 110:206–226CrossRefGoogle Scholar
  52. Scalbert A, Monties B, Janin G (1989) Tannins in wood: comparison of different estimation methods. J Agric Food Chem 37:1324–1329CrossRefGoogle Scholar
  53. Schultz TP, Nicholas DD (2000) Naturally durable heartwood: evidence for a proposed dual defensive function of extractives. Phytochemistry 54:47–52CrossRefGoogle Scholar
  54. Schultz TP, Nicholas DD, Kelly S (2006) A non-leachable waterborne composition of resin acids and wood preserving organic biocides US Provisional patent 60/743 669, filed 22Google Scholar
  55. Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, Heber D (2005) In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem 16:360–367CrossRefGoogle Scholar
  56. Sen S, Tascioglu C, Tırak K (2009) Fixation, leachability, and decay resistance of wood treated with some commercial extracts and wood preservative salts. Int Biodeterior Biodegrad 63:135–141CrossRefGoogle Scholar
  57. Singh T, Singh AP (2012) A review on natural products as wood protectant. Wood Sci Technol 46:851–870CrossRefGoogle Scholar
  58. Singh AP, Singh T (2014) Biotechnological applications of wood-rotting fungi: a review. Biomass Bioenergy 62:198–206CrossRefGoogle Scholar
  59. Sitheeque MAM, Panagoda GJ, Yau J, Amarakoon AM, Udagama UR, Samaranayake LP (2009) Antifungal activity of black tea polyphenols (catechins and theaflavins) against Candida species. Chemotherapy 55:189–196CrossRefGoogle Scholar
  60. Sotillo DR, Hadley M, Holm ET (1994) Phenolics in aqueous potato peel extract: extraction, identification and degradation. J Food Sci 59:649–651CrossRefGoogle Scholar
  61. Sultana B, Anwar F, Przybylski R (2007) Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees. Food Chem 104:1106–1114CrossRefGoogle Scholar
  62. Tascioglu C, Mesut Y, Selim S, Akcay C (2013) Antifungal properties of some plant extracts used as wood preservatives. Int Biodeterior Biodegrad 85:23–28CrossRefGoogle Scholar
  63. Taverna S, Corrado C (2017) Natural compounds: molecular weapons against Leukemia’s. J Leuk 5:1–7CrossRefGoogle Scholar
  64. Tehranifar A, Selahvarzi Y, Kharrazi M, Bakhsh VJ (2011) High potential of agro-industrial by-products of pomegranate (Punica granatum L.) as the powerful antifungal and antioxidant substances. Ind Crops Prod 34:1523–1527CrossRefGoogle Scholar
  65. Temiz A, Alfredsen G, Yildiz UC, Gezer ED, Kose G, Akbas S, Yildiz S (2014) Leaching and decay resistance of alder and pine wood treated with copper based wood preservatives. Maderas Cienc y Tecnol 16:63–76Google Scholar
  66. Tondi G, Wieland S, Lemenager N, Petutschnigg A, Pizzi A, Thevenon MF (2012) Efficacy of tannin in fixing boron in wood. Bioresources 7:1238–1252CrossRefGoogle Scholar
  67. Tondi G, Thevenon MF, Mies B, Standfest G, Petutschnigg A, Wieland S (2013) Impregnation of Scots pine and beech with tannin solutions: effect of viscosity and wood anatomy in wood infiltration. Wood Sci Technol 47:615–626CrossRefGoogle Scholar
  68. Wang W, Gao PJ (2003) Function and mechanism of a low-molecular-weight peptide produced by Gloeophyllum trabeum in biodegradation of cellulose. J Biotechnol 101:119–130CrossRefGoogle Scholar
  69. Witomski P, Olek W, Bonarski JT (2016) Changes in strength of Scots pine wood (Pinus silvestris L.) decayed by brown rot (Coniophora puteana) and white rot (Trametes versicolor). Constr Build Mater 102:162–166CrossRefGoogle Scholar
  70. Yamaguchi LH, Okuda K (1998) Chemically modified tannin and tannin–copper complexes as wood preservatives. Holzforschung 52:596–602CrossRefGoogle Scholar
  71. Zabalza BI, Valero Capilla AO, Aranda Usón A (2011) Life cycle assessment of building materials: comparative analysis of energy and environmental impacts and evaluation of the eco-efficiency improvement potential. Build Environ 46:1133–1140CrossRefGoogle Scholar
  72. Zahoor M, Manzoor A, Sumaira N, Musarrat A (2015) Cytotoxic, antibacterial and antioxidant activities of extracts of the bark of Melia Azedarach (China Berry). Nat Prod Res 29:1170–1172CrossRefGoogle Scholar
  73. Zhang Q, Shi Y, Liu XT, Liang JY, Ip NY, Min ZD (2007) Minor limonoids from Melia toosendan and their antibacterial activity. Planta Med 73:1298–1303CrossRefGoogle Scholar
  74. Zhang Z, Yang T, Mi N, Wang Y, Li G, Wang L, Xie Y (2016) Antifungal activity of monoterpenes against wood white-rot fungi. Int Biodeterior Biodegrad 106:157–160CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.CNRS/UNIV PAU & PAYS ADOUR, Institut des Sciences Analytiques et de Physicochimie pour l’Environnement et les Matériaux, XYLOMAT UMR 5254Mont de MarsanFrance
  2. 2.Unité de Recherche de Chimie Industrielle Organique et Alimentaire 00UR/1201, Institut National des Sciences Appliquées et de Technologie INSAT, Centre Urbain NordTunisTunisia

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