European Journal of Wood and Wood Products

, Volume 76, Issue 3, pp 1083–1085 | Cite as

Confocal spectral microscopy—an innovative tool for tracking of pathogen agents on contaminated wooden surfaces

  • L. Dubreil
  • F. Aviat
  • V. Anthoine
  • R. Ismail
  • A. Rossero
  • M. Federighi
Brief Original


Contamination of wood is an important consideration when this material is used as food packaging or as workplace surface. It is the reason why the choice of the raw material is important according to its properties related to retention of the bacteria or to inhibition of the bacterial invasion. It is therefore essential to develop a method able to detect and image bacteria on untreated wood surfaces allowing the assessment of the hygienic state of the material. In this study, a rapid non-destructive and sensitive method for detecting and locating bacteria on wood surfaces is described. Spectral confocal microscopy provides the high spatial and spectral resolution necessary to detect and image bacteria on wood.



Confocal spectral microscope LSM780 from Platform APEX UMR703 was acquired thanks to Pays de la Loire supports.


  1. Aviat F, Gerhards C, Rodriguez-Jerez JJ, Michel V, Bayon IL, Ismail R, Federighi M (2016) Microbial safety of wood in contact with food: a review. Comprehensive Reviews in Food Science Food Safety 15(3):491–505CrossRefGoogle Scholar
  2. Donaldson LA (2013) Softwood and hardwood lignin fluorescence spectra of wood cell walls in different mounting media. IAWA J 34:3–19CrossRefGoogle Scholar
  3. Ismaïl R, Aviat F, Michel V, Le Bayon I, Gay-Perret P, Kutnik M, Fédérighi M (2013) Methods for recovering microorganisms from solid surfaces used in the food industry: a review of the literature. Int J Environ Res Public Health 10(11):6169–6183CrossRefPubMedPubMedCentralGoogle Scholar
  4. Silva F, Lourenço O, Pina-Vaz C, Rodrigues AG, Queiroz JA, Domingues FC (2010) The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry. J Fluoresc 20(4):907–914CrossRefPubMedGoogle Scholar

Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.PAnTher, INRA, École nationale vétérinaire, agro-alimentaire et de l’alimentation Nantes-Atlantique (Oniris), Université Bretagne Loire (UBL)NantesFrance
  2. 2.YouR ResearcH Bio ScientificLe LandreauFrance
  3. 3.Oniris, UMR 1014 SECALIMNantesFrance
  4. 4.INRA, UMR 1014 SECALIMNantesFrance

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