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Bio-transformed sawdust by white rot fungi used as a carrier for plant growth-promoting bacteria

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Abstract

This study assessed the bio-transformation of sawdust wood waste by a white rot fungi consortium and explores the use of the final product as a carrier for plant growth-promoting bacteria. During 75 days, Tabebuia roseae and Eucalyptus pellita (1:1) sawdust wood were used as growing substrates for Ganoderma lucidum, Pleurotus ostreatus, Trametes versicolor and Phanerochaete chrysosporium. Then, bio-transformed sawdust was evaluated as carrier of two strains of Enterobacter sp. and one strain of Stenotrophomonas maltophilia. Biologic activity and viability were determined at two storage temperatures (23–4 °C) for 60 days. Sawdust mixture was bio-transformed by white rot fungi. After 45 days, the carbon/nitrogen ratio was reduced up to 46 %, and 4.8 mg/g of CO2 of residue was produced. Enzymatic activities attained a peak of 36.7 and 0.8 U g−1 for laccase and manganese peroxidase, respectively in 45 days. Population of plant growth-promoting bacteria immobilized in bio-transformed sawdust wood decreased to 105 CFU g−1. However, this concentration and its biologic activity remained stable at 23 °C.

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Acknowledgments

This research was supported and funded by Vicerrectoría Académica de la Pontificia Universidad Javeriana (Project 00004583) and COLCIENCIAS (Project 00005384). The authors thank Refocosta S.A.S for providing the sawdust waste used in this study. Authors thank Jorge Andres Fernandez Gonzales for English editing.

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Authors and Affiliations

  1. Unidad de Investigaciones Agropecuarias (UNIDIA), Laboratorio de Microbiología Ambiental y Suelos, Departamento de Microbiología, Pontificia Universidad Javeriana, Carrera 7 No 43-68 oficina 618, edificio 53, Bogotá, Colombia

    Naydu Shirley Rojas-Higuera, Ana Milena Pava-Sánchez, Diana Lizeth Pinzón Rangel & Aura Marina Pedroza-Rodríguez

  2. Grupo de Investigación en Agricultura Biológica. (GIAB), Laboratorio de Interrelación Suelos, Planta, Microorganismos, Departamento de Biología, Pontificia Universidad Javeriana, Bogotá, Colombia

    Lucía Ana Díaz-Ariza

  3. Grupos de Biotecnología Ambiental e Industrial (GBAI), Laboratorio de Biotecnología Aplicada, Departamento de Microbiología, Pontificia Universidad Javeriana, Bogotá, Colombia

    Balkys Quevedo-Hidalgo

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  1. Naydu Shirley Rojas-Higuera
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  2. Ana Milena Pava-Sánchez
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  3. Diana Lizeth Pinzón Rangel
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  5. Balkys Quevedo-Hidalgo
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  6. Aura Marina Pedroza-Rodríguez
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Correspondence to Aura Marina Pedroza-Rodríguez.

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Rojas-Higuera, N.S., Pava-Sánchez, A.M., Pinzón Rangel, D.L. et al. Bio-transformed sawdust by white rot fungi used as a carrier for plant growth-promoting bacteria. Eur. J. Wood Prod. 75, 263–273 (2017). https://doi.org/10.1007/s00107-016-1099-x

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