Zusammenfassung
Die Plasmamedizin hat sich in den letzten Jahren zu einem innovativen Forschungsgebiet mit großem Potenzial entwickelt. Seit der Entwicklung von Niedertemperaturplasmen stehen neue, fassettenreiche Anwendungsmöglichkeiten in der Medizin zur Verfügung. So hat sich eine multidisziplinäre Interessengruppe aus Medizinern, Physikern und Biologen gebildet, die gemeinsam versuchen, die Plasmamedizin zu verstehen und sowohl klinische als auch wissenschaftliche Fragestellungen zu beantworten. Für die Dermatologie werden neue Horizonte in der Wundheilung, Geweberegeneration, Behandlung von Hautinfektionen und Bekämpfung von Tumorerkrankungen eröffnet. Die größte Herausforderung bei der Einführung der Plasmamedizin in den klinischen Alltag wird es jedoch sein, die Kenntnis über die genauen Wirkmechanismen von Plasma auf Zellebene weiter zu vertiefen. Nur so kann eine sichere Anwendung von Plasma am Patienten gewährleistet werden.
Abstract
Plasma medicine has developed into an innovative field of research showing high potential. Since the establishment of cold atmospheric plasma, new, multifaceted medical treatment opportunities have become available. Within a short time a multidisciplinary special interest group of medical scientists, physicists, and biologists was created, aiming to understand plasma medicine and answer clinical as well as scientific questions. In dermatology, new horizons are being opened for wound healing, tissue regeneration, treatment of skin infections, and tumor therapy. A major task will be the introduction of plasma into clinical medicine and, simultaneously, the further investigation of the mechanisms of action of plasma at the cellular level. Only then can the safety of plasma treatment in patients be assured.
Literatur
Ahlfeld B, Li Y, Boulaaba A et al (2015) Inactivation of a foodborne norovirus outbreak strain with nonthermal atmospheric pressure plasma. MBio 6(1): e02300-14.
Alkawareek MY, Gorman SP, Graham WG et al (2014) Potential cellular targets and antibacterial efficacy of atmospheric pressure non-thermal plasma. Int J Antimicrob Agents 43:154–160
Arjunan KP, Friedman G, Fridman A et al (2012) Non-thermal dielectric barrier discharge plasma induces angiogenesis through reactive oxygen species. J R Soc Interface 9:147–157
Arndt S, Landthaler M, Zimmermann JL et al (2015) Effects of cold atmospheric plasma (CAP) on ss-defensins, inflammatory cytokines, and apoptosis-related molecules in keratinocytes in vitro and in vivo. PLoS One 10:e0120041
Arndt S, Unger P, Wacker E et al (2013) Cold atmospheric plasma (CAP) changes gene expression of key molecules of the wound healing machinery and improves wound healing in vitro and in vivo. PLoS One 8:e79325
Arndt S, Wacker E, Li YF et al (2013) Cold atmospheric plasma, a new strategy to induce senescence in melanoma cells. Exp Dermatol 22:284–289
Bekeschus S, Kolata J, Müller A, Kramer A, Weltmann KP, Bröker B, Masur K (2013) Differential viability of eight human blood mononuclear cell subpopulations after plasma treatment. Plasma Med 3:1–13
Bekeschus S, Masur K, Kolata J, Wende K, Schmidt A, Bundscherer L, Barton A, Kramer A, Bröker B, Weltmann KD (2013) Human mononuclear cell survival and proliferation is modulated by cold atmospheric plasma jet. Plasma Process Polym 10:706–713
Blackert S, Haertel B, Wende K et al (2013) Influence of non-thermal atmospheric pressure plasma on cellular structures and processes in human keratinocytes (HaCaT). J Dermatol Sci 70:173–181
Brehmer F, Haenssle HA, Daeschlein G et al (2015) Alleviation of chronic venous leg ulcers with a hand-held dielectric barrier discharge plasma generator (PlasmaDerm((R)) VU-2010): results of a monocentric, two-armed, open, prospective, randomized and controlled trial (NCT01415622). J Eur Acad Dermatol Venereol 29:148–155
Daeschlein G, Darm K, Niggemeier M, Majunke S et al (2009) Selective antistaphylococcal activity of atmospheric pressure plasma jet (APPJ) on human skin. Second International Conference on Plasma Medicine, San Antonio
Daeschlein G, Napp M, von Podewils S, Lutze S et al (2014) In vitro susceptibility of multidrug resistant skin and wound pathogens against low temperature atmospheric pressure plasma jet (APPJ) and dielectric barrier discharge plasma (DBD). Plasma Process Polym 11:175–183
Daeschlein G, Scholz S, Ahmed R et al (2012) Skin decontamination by low-temperature atmospheric pressure plasma jet and dielectric barrier discharge plasma. J Hosp Infect 81:177–183
Dobrynin D, Friedman G, Friedman G, Fridman A (2009) Physical and biological mechanisms of direct plasma interaction with living tissue. New J Phys 11:115–120
Fernandez A, Noriega E, Thompson A (2013) Inactivation of Salmonella enterica serovar Typhimurium on fresh produce by cold atmospheric gas plasma technology. Food Microbiol 33:24–29
Fridman A, Chirokov A, Gutsol A (2005) Non-thermal atmospheric pressure discharges. J Phys D Applied Phys 38 R1–R24
Graves D (2012) The emerging role of reactive oxy-gen and nitrogen species in redox biology and some implications for plasma applications to medicine and biology. J Phys D Appl Phys 45:263001
Grundmann H, Aires-de-Sousa M, Boyce J et al (2006) Emergence and resurgence of meticillin-resistant Staphylococcus aureus as a public-health threat. Lancet 368:874–885
Haertel B, Strassenburg S, Oehmigen K et al (2013) Differential influence of components resulting from atmospheric-pressure plasma on integrin expression of human HaCaT keratinocytes. Biomed Res Int 2013:761451
Haertel B, Volkmann F, von Woedtke T et al (2012) Differential sensitivity of lymphocyte subpopulations to non-thermal atmospheric-pressure plasma. Immunobiology 217:628–633
Haertel B, Wende K, von Woedtke T et al (2011) Non-thermal atmospheric-pressure plasma can influence cell adhesion molecules on HaCaT-keratinocytes. Exp Dermatol 20:282–284
Heinlin J, Isbary G, Stolz W et al (2011) Plasma applications in medicine with a special focus on dermatology. J Eur Acad Dermatol Venereol 25:1–11
Heinlin J, Isbary G, Stolz W et al (2013) A randomized two-sided placebo-controlled study on the efficacy and safety of atmospheric non-thermal argon plasma for pruritus. J Eur Acad Dermatol Venereol 27:324–331
Heinlin J, Maisch T, Zimmermann JL et al (2013) Contact-free inactivation of Trichophyton rubrum and Microsporum canis by cold atmospheric plasma treatment. Future Microbiol 8:1097–1106
Heinlin J, Zimmermann JL, Zeman F et al (2013) Randomized placebo-controlled human pilot study of cold atmospheric argon plasma on skin graft donor sites. Wound Repair Regen 21:800–807
Hirst AM, Simms MS, Mann VM et al (2015) Low-temperature plasma treatment induces DNA damage leading to necrotic cell death in primary prostate epithelial cells. Br J Cancer 112:1536–1545
Hoentsch M, Woedtke von T, Weltmann KD, Nebe JB (2012) Time-dependent effects of low-temperature atmospheric-pressure argon plasma on epithelial cell attachment, viability and tight junction formation in vitro. J Phys D Appl Phys. doi:10.1088/0022-3727/45/2/025206
Hong YF, Kang JG, Lee HY et al (2009) Sterilization effect of atmospheric plasma on Escherichia coli and Bacillus subtilis endospores. Lett Appl Microbiol 48:33–37
Isbary G, Heinlin J, Shimizu T et al (2012) Successful and safe use of 2 min cold atmospheric argon plasma in chronic wounds: results of a randomized controlled trial. Br J Dermatol 167:404–410
Isbary G, Morfill G, Schmidt HU et al (2010) A first prospective randomized controlled trial to decrease bacterial load using cold atmospheric argon plasma on chronic wounds in patients. Br J Dermatol 163:78–82
Isbary G, Morfill G, Zimmermann J et al (2011) Cold atmospheric plasma: a successful treatment of lesions in Hailey-Hailey disease. Arch Dermatol 147:388–390
Isbary G, Shimizu T, Zimmermann JL et al (2013) Cold atmospheric plasma for local infection control and subsequent pain reduction in a patient with chronic post-operative ear infection. New Microbes New Infect 1:41–43
Isbary G, Shimizu T, Zimmermann JL, Heinlin J, Al-Zaabi S, Rechfeld M, Morfill GE, Karrer S, Stolz M (2014) Randomized placebo-controllled clinical trial showed cold atmospheric argon plasma in herpes zoster relieved acute pain and accelerated healing. Clin Plasma Med J 2:50–55
Isbary G, Stolz W, Shimizu T, Monetti R, Bunk W, Schmidt H-U, Morfill GE, Klämpfl TG, Steffes B, Thomas HM, Heinlin J, Karrer S, Landthaler M, Zimmermann JL (2013) Cold atmospheric argon plasma treatment may accelerate wound healing in chronic wounds: results of an open retrospective randomized controlled study in vivo. Clin Plasma Med J 1:25–30
Ishaq M, Evans MM, Ostrikov KK (2014) Effect of atmospheric gas plasmas on cancer cell signaling. Int J Cancer 134:1517–1528
Kalghatgi S, Friedman G, Fridman A et al (2010) Endothelial cell proliferation is enhanced by low dose non-thermal plasma through fibroblast growth factor-2 release. Ann Biomed Eng 38:748–757
Kalghatgi S, Kelly CM, Cerchar E et al (2011) Effects of non-thermal plasma on mammalian cells. PLoS One 6:e16270
Kieft IE, Broers JL, Caubet-Hilloutou V et al (2004) Electric discharge plasmas influence attachment of cultured CHO K1 cells. Bioelectromagnetics 25:362–368
Kim CH, Bahn JH, Lee SH et al (2010) Induction of cell growth arrest by atmospheric non-thermal plasma in colorectal cancer cells. J Biotechnol 150:530–538
Kim JY, Ballato J, Foy P et al (2011) Apoptosis of lung carcinoma cells induced by a flexible optical fiber-based cold microplasma. Biosens Bioelectron 28:333–338
Klein E, Smith DL, Laxminarayan R (2007) Hospitalizations and deaths caused by methicillin-resistant Staphylococcus aureus, United States, 1999–2005. Emerg Infect Dis 13:1840–1846
Maisch T, Shimizu T, Isbary G et al (2012) Contact-free inactivation of Candida albicans biofilms by cold atmospheric air plasma. Appl Environ Microbiol 78:4242–4247
Maisch T, Shimizu T, Li YF et al (2012) Decolonisation of MRSA, S. aureus and E. coli by cold-atmospheric plasma using a porcine skin model in vitro. PLoS One 7:e34610
Maisch T, Shimizu T, Mitra A et al (2012) Contact-free cold atmospheric plasma treatment of Deinococcus radiodurans. J Ind Microbiol Biotechnol 39:1367–1375
Marschewski M, Hirschberg J, Omairi T et al (2012) Electron spectroscopic analysis of the human lipid skin barrier: cold atmospheric plasma-induced changes in lipid composition. Exp Dermatol 21:921–925
Mertens N, Helmke A, Goppold A, Emmert S, Kaemling A, Wandke D, Vioel W (2009) Low temperature plasma treatment of human tissue. Second International Conference on Plasma Medicine, San Antonio
Mertens N, Helmke A, Vioel W (2009) Dielectric barrier discharge plasma – an upcoming approach in skin treatment. 2nd International Workshop on Plasma-Tissue Interactions, Greifswald
Metelmann HR, Vu TT, Do HT et al (2013) Scar formation of laser skin lesions after cold atmospheric pressure plasma (CAP) treatment: a clinical long term observation. Clin Plasma Med 1:30–35
Niemira BA (2012) Cold plasma reduction of Salmonella and Escherichia coli O157:H7 on almonds using ambient pressure gases. J Food Sci 77:M171–M175
Niemira BA, Sites J (2008) Cold plasma inactivates Salmonella Stanley and Escherichia coli O157:H7 inoculated on golden delicious apples. J Food Prot 71:1357–1365
Park SB, Kim B, Bae H et al (2015) Differential epigenetic effects of atmospheric cold plasma on MCF-7 and MDA-MB-231 breast cancer cells. PLoS One 10:e0129931
Schmidt A, Wende K, Bekeschus S et al (2013) Non-thermal plasma treatment is associated with changes in transcriptome of human epithelial skin cells. Free Radic Res 47:577–592
Shashurin A, Stepp M, Hawley TS, Pal-Ghosh S, Brieda L, Bronnikov S, Jurjus RA, Keidar M (2010) Influence of cold plasma atmospheric jet on surface integrin expression of living cells. Plasma Process Polym 7:294–300
Siu A, Volotskova O, Cheng X et al (2015) Differential effects of cold atmospheric plasma in the treatment of malignant glioma. PLoS One 10:e0126313
Spange S, Pfuch A, Wiegand C et al (2015) Atmospheric pressure plasma CVD as a tool to functionalise wound dressings. J Mater Sci Mater Med 26:76
Ulrich C, Kluschke F, Patzelt A, et al (2015) Clinical use of cold atmospheric pressure argon plasma in chronic leg ulcers: A pilot study. J Wound Care 24:(5):196–203
Wende K, Strassenburg S, Haertel B et al (2014) Atmospheric pressure plasma jet treatment evokes transient oxidative stress in HaCaT keratinocytes and influences cell physiology. Cell Biol Int 38:412–425
Wiegand C, Beier O, Horn K et al (2014) Antimicrobial impact of cold atmospheric pressure plasma on medical critical yeasts and bacteria cultures. Skin Pharmacol Physiol 27:25–35
Wu Y, Liang Y, Wei K et al (2014) Rapid allergen inactivation using atmospheric pressure cold plasma. Environ Sci Technol 48:2901–2909
Zimmermann JL, Dumler K, Shimizu T, Morfill GE, Wolf A, Boxhammer V, Schlegel J, Gansbacher B, Anton M (2011) Effects of cold atmospheric plasmas on adenoviruses in solution. J Phys D Appl Phys 44:505201
Ziuzina D, Patil S, Cullen PJ et al (2013) Atmospheric cold plasma inactivation of Escherichia coli in liquid media inside a sealed package. J Appl Microbiol 114:778–787
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Interessenkonflikt
S. Karrer und S. Arndt geben an, dass kein Interessenkonflikt besteht.
Dieser Beitrag beinhaltet keine Studien an Menschen oder Tieren.
Rights and permissions
About this article
Cite this article
Karrer, S., Arndt, S. Plasmamedizin in der Dermatologie. Hautarzt 66, 819–828 (2015). https://doi.org/10.1007/s00105-015-3686-x
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00105-015-3686-x