Dipeptides as linker for multicomponent presentation—a facile, robust, and high-bioactivity yielding strategy
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The need for multiple drugs arises when the response to pharmacological inflection is complicated and the disease conditions get worsened. The treatment requires a common platform to which multiple drugs are linked and this methodology is proven to be useful in drug delivery, biomaterial research, biomedicine, and vaccine development. In view of this, the present work is centered on dipeptides (KW/ KD/ KE/ kW/ kD/ kE), which are used as linkers and can potentially hold different functional group containing molecules (here, amino and carboxyl). First, we have demonstrated the incorporation of three components to the linker successfully followed by tetra component linking to those dipeptides, which contain trifunctional groups. To provide a proof of concept, these multisubstituted constructs were subjected to microbial growth suppression assay as well as anti-inflammatory assay. The biological results revealed that the multimers play a key role in enhancing the activity. Hence, the present system may be regarded as simple and straight forward which can be employed to develop various therapeutic agents as well as in different methodologies.
KeywordsMulticomponent Dipeptides Linker Bioactivity
- QZN 1
- QZN 2
We gratefully acknowledge Department of Science and Technology (DST) New Delhi for awarding Inspire Fellowship, University Grant Commission (UGC) New Delhi for awarding BSR faculty fellowship and UGC-Post doctoral Fellowship (PDFSS). We also acknowledge DST-Purse and Instrumentation facility.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Mezo G, de Oliveira E, Krikorian D, Feijlbrief M, Jakab A, Tsikaris V, Sakarellos C, Welling-Wester S, Andreu D, Hudecz F (2003) Synthesis and comparison of antibody recognition of conjugates containing herpes simplex virus type 1 glycoprotein D epitope VII. Bioconjugate Chem 14:1260–1269CrossRefGoogle Scholar
- Mozdzanowska K, Feng J, Eid M, Kragol G, Cudic Jr. M, Otvos L, Gerhard W (2003) Induction of influenza type A virus-specific resistance by immunization of mice with a synthetic multiple antigenic peptide vaccine that contains ectodomains of matrix protein 2. Vaccine 21:2616–2626CrossRefPubMedGoogle Scholar
- Perez C, Paul M, Bazerque P (1990) An antibiotic assay by the agar-well diffusion method. Acta Biol Med Exp 15:113–115Google Scholar
- Singh I, Singh VP (2000) Antifungal properties of aqueous and organic solution extracts of seed plants against Aspergillus flavus and A. niger. Phytomorphology 50:151–157Google Scholar