Tonifying qi and Warming yang, a compound traditional Chinese herbal medicine, suppresses AngII-induced cardiac H9c2 cells apoptosis through regulating PI3K/Akt signaling pathway
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This study aims to evaluate the effects of Tonifying qi and Warming yang on Ang II-induced cardiac apoptosis and mitochondrial dysfunction and to explore the underlying mechanism. Sprague Dawley (SD) rats were treated with Tonifying qi and Warming yang, and the drug-contained serum was used in our experiment. Ang II-treated H9c2 cells were administered with drug-contained serum in different concentrations. The cell viability was determined by MTT and cell apoptosis was analyzed by the Flow cytometry instrument. The changes of mitochondrial membrane potential (MMP) was detected by Rho123 assay. qRT-PCR, Western blot and immunocytochemical staining were used to measure the mRNA and protein expression levels, respectively. Our results showed that drug-contained serum treatment prevented AngII-induced cell viability inhibition, suppressed AngII-induced apoptosis and prevented AngII-induced MMP reduction. Mechanically, drug-contained serum treatment prevented AngII-induced apoptosis by regulating expression of apoptosis-related genes, Bcl-2, Bax, Cyt-c, caspase-3, and PI3K. Besides, we found LY29400, an inhibitor of PI3K, reversed the effects of drug-contained serum treatment on apoptosis-related genes (p-AKT, Bcl-2, and Bax) in AngII-treated H9c2 cells. Totally, Tonifying qi and Warming yang, a compound traditional Chinese herbal medicine, suppresses AngII-induced cardiac H9c2 cells apoptosis through regulating PI3K/Akt signaling pathway.
KeywordsTonifying qi and Warming yang Chinese herbal medicine AngII PI3K/Akt signaling pathway
This research was supported by grants from the Key Laboratory of Myocardial (Harbin Medical University), Chinese Ministry of Education.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no competing interests.
- Chang YM, Tsai CT, Wang CC, Chen YS, Lin YM, Kuo CH, Tzang BS, Chen RJ, Tsai FJ, Huang CY (2013) Alpinate oxyphyllae fructus (Alpinia Oxyphylla Miq) extracts inhibit angiotensin-II induced cardiac apoptosis in H9c2 cardiomyoblast cells. Agric Biol Chem 77:229–234Google Scholar
- Chen LX, Liao JZ, Guo WQ, Yu WJ, Qin LM (1996) Effects of Radix Astragali on left ventricular function and oxygen free radicals in acute myocardial infarction patients and its mechanism of cardiotonic action. Chin J Integr Med 2:252–254Google Scholar
- Dickstein K, CohenSolal A, Filippatos G, McMurray JJ, Ponikowski P (2008) ESC guidelines for the diagnosis and treatment of acute and chronic heart failure. Eur J Heart Fail 10:933–989Google Scholar
- Dong Q, Ding SW (2013) Effect of Renshen Jianxin capsule on neuroendocrine activation in rat congestive heart failure caused by doxorubicin. Chin J Exp Tradit Med Formulae 19:243–246Google Scholar
- Francis GS, Cohn JN (1990) Heart failure: mechanisms of cardiac and vascular dysfunction and the rationale for pharmacologic intervention. Faseb J Off Publ Fed Am Soc Exp Biol 4:3068–3075Google Scholar
- Han L, Wang Z, Chai S, Xing G, Wang H, Yuan B, Li Z (2016) Medicinals with properties of warming Yang and tonifying Qi in terms of Traditional Chinese Medicine: their effects on left ventricular ejection fraction and aldosterone in rats with induced failure heart. J Tradit Chin Med 36:789–793CrossRefGoogle Scholar
- Hunt SA (2001) ACC/AHA 2005 Guideline Update for the Diagnosis and Management of Chronic Heart Failure in the Adult: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Writing Committee to Update the 2001 Guidelines. J Am Coll Cardiol 38:2101–2113CrossRefPubMedGoogle Scholar
- Jianying WU (2012) Clinical observation of warming Yang,Tonifying Qi and Channelizing Body Fluid Method to Treat Refractory Heart Failure. J Sichuan Tradit Chin Med 3:76–77Google Scholar
- National Institute for Clinical Excellence (2003) Chronic heart failure. Management of chronic heart failure in adults in primary and secondary care. Clinical Guidelines. Vol. 5. London: NICEGoogle Scholar
- Sun W, Wang H, Huo Y, Jie YU, Zhou J, Yingming MA (2015) Optimal effectiveness research on chronic heart failure with tonifying qi for warming yang and promoting blood circulation for diuresis herbs based on regular treatment. Modern Chinese Clinical Medicine 22:5–8Google Scholar
- Wang TXY, Wang SQ (2011) Clinical observation of shenfuqiangxin granule in treating chronic heart failure. Zhong Guo Zhong Yi Ji Zheng 20:1749–1765Google Scholar
- Yan HCX, Lu Q, Liu YM, Chen QY, Cai HZ (2013) Effect of Wenyangzhenghuai decoction on myocardial matrix metalloproteinase - 2, 9 in rats with chronic heart failure. Guang Ming Zhong Yi 28:1336–1339Google Scholar
- Zheng J (2011) Clinical observation of method of Tonifying Qi to Warm Yang and promote blood flow on sinus bradycardia. Shanxi J Tradit Chin Med 27:13–14Google Scholar