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Isolation, characterization and antiproliferative evaluation of constituents from stem extracts of Alafia barteri Oliv. Hook. F.

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An Author Correction to this article was published on 26 December 2017

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Abstract

This study was to investigate the constituents of stem extracts of Alafia barteri and evaluate its antiproliferative activity in order to support its ethnomedicinal application. Chromatographic isolation procedure was used to separate the constituents from n-hexane, ethyl acetate, and methanol extracts of Alafia barteri stem and testing against three human cancer cell lines, i.e., leukemia carcinoma, K-562, hepatic liver cancer cells, WRL68, and breast carcinoma, MCF-7 using the MTT assay. Thirteen known fatty esters: 1,2,3-propanetriyl tris(dodecanoate) (1), 1,2,3-propanetriyl tris(tetradecanoate) (2), 1,2,3-propanetriyl tris(pentadecanoate) (3), 1,2,3-propanetriyl (5Z,5′Z,5″Z)tris(-5-pentadecenoate) (4), 1,2,3-propanetriyl (7Z,7′Z,7″Z)tris(-7-pentadecenoate) (5), 1,2,3-propanetriyl tris(hexadecanoate) (6), 1,2,3-propanetriyl (9Z,9′Z,9″Z)tris(-9-hexadecenoate) (7), 1,2,3-propanetriyl (9Z,9′Z,9″Z)tris(-9-octadecenoate) (8), methyl octanoate (9), 9Z-ethyloctadec-9-enoate (10), 1,2,3-propanetriyl (5Z,5′Z,5″Z)tris(-5-eicosenoate) (11), dodec-5-enoic acid (12) and 1,2,3-propanetriyl (9Z,12′Z,15″Z)tris(-9,12,15-uneicostrienoate) (13); an isoalcohol, 12-methyltridecan-2-ol (14), β-sitosterol (15), lupeol (16), and α-amyrin (17) were isolated from the extracts and characterized using infrared, nuclear magnetic resonance, and mass spectroscopic techniques. Compounds 2 and 3 showed cytotoxic activity against WRL68 cell lines (IC50 = 63.50 and 65.03 µM, respectively), while 5 exhibited cytotoxic activity against MCF-7 (IC50 = 85.26 µM). Compounds 9, 12, and 16 inhibited the growth of K-562 carcinoma with IC50 of 28.31, 52.32, and 85.20 µM, respectively. The phytochemical constituents of Alafia barteri stem extracts could be considered as lead compounds for further investigation as antiproliferative agents.

Graphical abstract

Antiproliferative activity………

WRL68 Compounds 2 and 3: IC50 = 63.50 ± 7.07 × 10−3 and 65.03 ± 7.07 × 10−3 µM. MCF-7 Compound 5: IC50 = 85.26 ± 1.41 × 10−2 µM.

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Change history

  • 26 December 2017

    Correction to: https://doi.org/10.1007/s00044-017-2033-4

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Acknowledgements

We are thankful to Director, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India for rendering essential research facilities and support. TWAS-CSIR Postgraduate fellowship (Sandwich, 2012–2013) to A.A. Hamid is duly acknowledged. A.A. Hamid also acknowledged the support of University of Ilorin, Nigeria.

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Authors and Affiliations

  1. Department of Chemistry, University of Ilorin, Ilorin, Kwara State, Nigeria

    Abdulmumeen A. Hamid

  2. Department of Chemistry, University of Ibadan, Ibadan, Oyo State, Nigeria

    Olapeju O. Aiyelaagbe

  3. Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, India

    Fatima Kaneez, Suaib Luqman & Arvind S. Negi

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  1. Abdulmumeen A. Hamid
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  2. Olapeju O. Aiyelaagbe
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  5. Arvind S. Negi
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Correspondence to Abdulmumeen A. Hamid.

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A correction to this article is available online at https://doi.org/10.1007/s00044-017-2116-2.

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Hamid, A.A., Aiyelaagbe, O.O., Kaneez, F. et al. Isolation, characterization and antiproliferative evaluation of constituents from stem extracts of Alafia barteri Oliv. Hook. F. . Med Chem Res 26, 3407–3416 (2017). https://doi.org/10.1007/s00044-017-2033-4

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