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Versatile cell ablation tools and their applications to study loss of cell functions

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Abstract

Targeted cell ablation is a powerful approach for studying the role of specific cell populations in a variety of organotypic functions, including cell differentiation, and organ generation and regeneration. Emerging tools for permanently or conditionally ablating targeted cell populations and transiently inhibiting neuronal activities exhibit a diversity of application and utility. Each tool has distinct features, and none can be universally applied to study different cell types in various tissue compartments. Although these tools have been developed for over 30 years, they require additional improvement. Currently, there is no consensus on how to select the tools to answer the specific scientific questions of interest. Selecting the appropriate cell ablation technique to study the function of a targeted cell population is less straightforward than selecting the method to study a gene’s functions. In this review, we discuss the features of the various tools for targeted cell ablation and provide recommendations for optimal application of specific approaches.

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Funding

This work was supported by R21OD024931 (XQ), R21AA024984 (SLC and XQ), R21 DA043448 (SLC and XQ), R01 HL130233 (XQ), R01HL141132 (XQ) and P40 OD011062 (ECB).

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Authors and Affiliations

  1. Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N Broad Street, Philadelphia, PA, 19140, USA

    Fengming Liu, Shen Dai, Xiao Peng, Zhongnan Qin, Alison C. Kearns, Yong Chen, Hong Wang & Xuebin Qin

  2. Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA, 70433, USA

    Fengming Liu, Zhongnan Qin & Xuebin Qin

  3. Department of Immunology and Microbiology, Tulane University School of Medicine, New Orleans, LA, 70112, USA

    Fengming Liu, Zhongnan Qin & Xuebin Qin

  4. Laboratory of Liver Diseases, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD, 20892, USA

    Dechun Feng & Bin Gao

  5. Institute of NeuroImmune Pharmacology, Seton Hall University, 400 South Orange Avenue, South Orange, NJ, 07079, USA

    Wenfei Huang & Sulie L. Chang

  6. Key Lab for Immunology in Universities of Shandong Province, School of Clinical Medicine, Weifang Medical University, 261053, Weifang, People’s Republic of China

    Yong Chen

  7. Institute of Anatomy and Cell Biology, Julius-Maximillan University, 97070, Wurzburg, Germany

    Süleyman Ergün

  8. Division of Pathology, Tulane National Primate Research Center, 18703 Three Rivers Road, Covington, LA, 70433, USA

    Jay Rappaport

  9. Rat Resource and Research Center, University of Missouri, 4011 Discovery Drive, Columbia, MO, 65201, USA

    Elizabeth C. Bryda

  10. Division of Biological Sciences, 340D Life Sciences Center, University of Missouri, 1201 Rollins St, Columbia, MO, USA

    Anand Chandrasekhar

  11. Department of Medicine, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, 02115, USA

    Bertal Aktas

  12. Department of Anesthesiology, Center for the Study of Itch, Washington University School of Medicine, St. Louis, MO, 63110, USA

    Hongzhen Hu

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  1. Fengming Liu
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  2. Shen Dai
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  3. Dechun Feng
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  4. Xiao Peng
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  9. Süleyman Ergün
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  10. Hong Wang
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  15. Hongzhen Hu
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  16. Sulie L. Chang
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  17. Bin Gao
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  18. Xuebin Qin
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Contributions

FL, SD, DF, XP, ZQ, ACK, WH, YC, SE, HW, JR, ECB, AC, BA, HH, SLC, BG, and XQ wrote the paper. ZQ and XB drew the pictures and generated table. XB developed the idea.

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Correspondence to Xuebin Qin.

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Liu, F., Dai, S., Feng, D. et al. Versatile cell ablation tools and their applications to study loss of cell functions. Cell. Mol. Life Sci. 76, 4725–4743 (2019). https://doi.org/10.1007/s00018-019-03243-w

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  • DOI: https://doi.org/10.1007/s00018-019-03243-w

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