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Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations

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Abstract

The P3H1/CRTAP/PPIB complex is essential for prolyl 3-hydroxylation and folding of procollagens in the endoplasmic reticulum (ER). Deficiency in any component of this ternary complex is associated with the misfolding of collagen and the onset of osteogenesis imperfecta. However, little structure information is available about how this ternary complex is assembled and retained in the ER. Here, we assessed the role of the KDEL sequence of P3H1 and probed the spatial interactions of PPIB in the complex. We show that the KDEL sequence is essential for retaining the P3H1 complex in the ER. Its removal resulted in co-secretion of P3H1 and CRTAP out of the cell, which was mediated by the binding of P3H1 N-terminal domain with CRTAP. The secreted P3H1/CRTAP can readily bind PPIB with their C-termini close to PPIB in the ternary complex. Cysteine modification, crosslinking, and mass spectrometry experiments identified PPIB surface residues involved in the complex formation, and showed that the surface of PPIB is extensively covered by the binding of P3H1 and CRTAP. Most importantly, we demonstrated that one disease-associated pathological PPIB mutation on the binding interface did not affect the PPIB prolyl-isomerase activity, but disrupted the formation of P3H1/CRTAP/PPIB ternary complex. This suggests that defects in the integrity of the P3H1 ternary complex are associated with pathological collagen misfolding. Taken together, these results provide novel structural information on how PPIB interacts with other components of the P3H1 complex and indicate that the integrity of P3H1 complex is required for proper collagen formation.

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Abbreviations

ER:

Endoplasmic reticulum

P3H1:

Prolyl 3-hydroxylase 1

CRTAP:

Cartilage-associated protein

PPIB:

Peptidyl–prolyl cis–trans isomerase B

OI:

Osteogenesis imperfecta

mPEG:

NEM-PEG2000

MW:

Molecular weight

HC:

Hyperelastosis cutis

WT:

Wild type

IPTG:

Isopropyl-β-d-thiogalactopyranoside

DTT:

Dithiothreitol

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Acknowledgements

This work was supported in part by Grants from the National Natural Science Foundation of China (81870309, 31570824, and 81572090), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Shanghai PuJiang Program, and Innovation Program of Shanghai Municipal Education Commission (no. 12ZZ113).

Funding

This work was supported in part by Grants from the National Natural Science Foundation of China (81870309, 31570824, and 81572090), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning, Shanghai PuJiang Program, and Innovation Program of Shanghai Municipal Education Commission (no. 12ZZ113).

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  1. Jiawei Wu, Wenting Zhang, and Li Xia have contributed equally to this work.

Authors and Affiliations

  1. Department of Pathophysiology, Shanghai Tongren Hospital/Faculty of Basic Medicine, Hongqiao International Institute of Medicine; Key Laboratory of Cell Differentiation and Apoptosis of the Chinese Ministry of Education, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China

    Jiawei Wu, Wenting Zhang, Li Xia, Lingling Feng, Zimei Shu, Jing Zhang, Naiyan Zeng & Aiwu Zhou

  2. Department of Preventive Dentistry, The Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China

    Wei Ye

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  1. Jiawei Wu
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Contributions

JW and WZ prepared all the P3H1/CRTAP/PPIB mutants. WZ, ZS, JZ, and LF performed the in vitro experiment about the interaction of P3H1 and CRTAP. J.W carried out the PPIB cysteine modification and crosslinking experiment. XL helped to establish the mass spectrometry method. JW and AZ wrote the paper. WY, NZ, and AZ conceived and designed all the experiments.

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Correspondence to Wei Ye, Naiyan Zeng or Aiwu Zhou.

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Wu, J., Zhang, W., Xia, L. et al. Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations. Cell. Mol. Life Sci. 76, 3899–3914 (2019). https://doi.org/10.1007/s00018-019-03102-8

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