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Cellular and Molecular Life Sciences

, Volume 75, Issue 15, pp 2843–2856 | Cite as

Stem cells from human apical papilla decrease neuro-inflammation and stimulate oligodendrocyte progenitor differentiation via activin-A secretion

  • Pauline De Berdt
  • Pauline Bottemanne
  • John Bianco
  • Mireille Alhouayek
  • Anibal Diogenes
  • Amy Llyod
  • Jose Gerardo-Nava
  • Gary A. Brook
  • Véronique Miron
  • Giulio G. Muccioli
  • Anne des Rieux
Original Article

Abstract

Secondary damage following spinal cord injury leads to non-reversible lesions and hampering of the reparative process. The local production of pro-inflammatory cytokines such as TNF-α can exacerbate these events. Oligodendrocyte death also occurs, followed by progressive demyelination leading to significant tissue degeneration. Dental stem cells from human apical papilla (SCAP) can be easily obtained at the removal of an adult immature tooth. This offers a minimally invasive approach to re-use this tissue as a source of stem cells, as compared to biopsying neural tissue from a patient with a spinal cord injury. We assessed the potential of SCAP to exert neuroprotective effects by investigating two possible modes of action: modulation of neuro-inflammation and oligodendrocyte progenitor cell (OPC) differentiation. SCAP were co-cultured with LPS-activated microglia, LPS-activated rat spinal cord organotypic sections (SCOS), and LPS-activated co-cultures of SCOS and spinal cord adult OPC. We showed for the first time that SCAP can induce a reduction of TNF-α expression and secretion in inflamed spinal cord tissues and can stimulate OPC differentiation via activin-A secretion. This work underlines the potential therapeutic benefits of SCAP for spinal cord injury repair.

Keywords

Spinal cord Dental stem cells Inflammation Oligodendrocyte progenitor cells Differentiation 

Notes

Acknowledgements

Anne des Rieux is a Research Associate, Mireille Alhouayek is a Post-doctoral Researcher and Pauline Bottemanne is a FRIA Doctoral Researcher at the FRS-FNRS (Fonds de la Recherche Scientifique). The authors acknowledge Prof. O. Feron (UCL) for the access to hypoxia incubator and Daniel Soong (EdU) for his help with MBP quantification as well as Loïc Germain (UCL) for his support in the development of the tri-cultures. We are also grateful to Université Catholique de Louvain (FSR) and International Foundation for Research in Paraplegia (IRP) for the financial support. The authors declare no potential conflicts of interest with respect to the authorship and/or publication of this article.

Supplementary material

18_2018_2764_MOESM1_ESM.tiff (960 kb)
Supplementary material 1 (TIFF 960 kb)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Pauline De Berdt
    • 1
  • Pauline Bottemanne
    • 2
  • John Bianco
    • 1
  • Mireille Alhouayek
    • 2
  • Anibal Diogenes
    • 3
  • Amy Llyod
    • 4
  • Jose Gerardo-Nava
    • 5
  • Gary A. Brook
    • 5
  • Véronique Miron
    • 4
  • Giulio G. Muccioli
    • 2
  • Anne des Rieux
    • 1
  1. 1.Louvain Drug Research Institute (LDRI), Advanced Drug Delivery and Biomaterials (ADDB)Université Catholique de LouvainBrusselsBelgium
  2. 2.Louvain Drug Research Institute, Bioanalysis and Pharmacology of Bioactive Lipids Research Group (BPBL)Université Catholique de LouvainBrusselsBelgium
  3. 3.Department of EndodonticsUniversity of Texas Health Science Center at San AntonioTexasUSA
  4. 4.MRC Center for Reproductive Health, The Queen’s Medical Research InstituteThe University of EdinburghEdinburghUK
  5. 5.Institute of NeuropathologyUniklinik RWTH AachenAachenGermany

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