Fig. 6 | Cellular and Molecular Life Sciences

Fig. 6

From: Bafilomycin A1 activates respiration of neuronal cells via uncoupling associated with flickering depolarization of mitochondria

Fig. 6

The interplay between Baf and Ca2+ in dPC12 cell. a Cytosolic Ca2+ levels increase dramatically after 45–60 min of treatment with 0.25 μM Baf, as detected with the Fluo-4 probe. b Uncoupling with 0.5 μM FCCP causes a dramatic drop in mitoCase12 fluorescence in Baf(+) cells, indicating a deep decrease in mitochondrial Ca2+, similar to that seen in the control. c The effect of Baf on cell respiration is abolished by increasing the Ca2+ buffering capacity of the cytosol with 25 μM BAPTA-AM or by inhibiting the SERCA with 10 μM thapsigargin. The respiratory response is not changed under extracellular Ca2+-free conditions, or upon inhibition of plasma membrane Ca2+-ATPase with caloxin 1C2 (100 μM). It is not significantly downregulated by the inhibition of mitochondrial Na+/Ca2+ exchanger with CGP37157 (10 μM). DMSO was used as a negative control. Bars 20 μm; asterisks significant differences

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