Fig. 3 | Cellular and Molecular Life Sciences

Fig. 3

From: Bafilomycin A1 activates respiration of neuronal cells via uncoupling associated with flickering depolarization of mitochondria

Fig. 3

Baf activates glycolysis and reduces ATP production by OxPhos in dPC12 cells. a In the cells treated with 0.25 μM Baf and CMA for 30 min, cytosolic pH decreases. Live-cell imaging of the BCECF distribution within the cells reveals focal condensation of the probe. b Extracellular acidification rate (ECA), which is linked to the rate of glycolysis, increases in the cells treated with 0.25 μM Baf to the levels characteristic of valinomycin (Val, 0.25 μM) or FCCP (FCCP, 0.5 μM) uncoupling. CMA (0.25 μM) does not change the extracellular acidification. c, d dPC12 cells grown in galactose(+) conditions show a decrease in their ATP levels in a Baf concentration-dependent manner (c) and a time-dependent manner (d). In glucose(+) medium Baf does not affect cellular ATP levels. Inhibition of the F0F1 ATPase by oligomycin (10 μM) further reduces the ATP levels. DMSO was used as a negative control. Asterisks significant differences

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