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Inflammation Research

, Volume 49, Issue 9, pp 452–459 | Cite as

Involvement of the β 2-integrin CD18 in apoptosis signal transduction in human neutrophils

  • J. Larsson
  • L. Serrander
  • O. Stendahl
  • H. Lundqvist-Gustafsson
Article

Abstract:

Objective and design: To examine the hypothesis that an accelerated rate of neutrophil apoptosis occurs following β 2-integrin activation, and further investigate the signal transduction pathways involved.¶Material: Human polymorphonuclear neutrophils.¶Treatment: Neutrophils were challenged with pansorbins coated with antibodies towards the β 2-integrin subunit CD18 in a proportion of 1:100 with or without the inhibitors diphenylene iodonium (10 M), cytochalasin B (5 μg/ml), genistein (10 nM), herbimycin A (10 M) and Z-VAD-FMK (10 μM).¶Methods: Measurement of phosphatidylserine exposure and DNA fragmentation in flow cytometry and assessment of H2O2-production through spectrofluorometry. The results were analysed using Mann Whitney U test and Kruskal Wallis.¶Results: Pansorbins coated with antibodies to CD18 induce apoptosis in neutrophils (p<0.01), and activate the production of reactive oxygen species (p<0.01). Pre-treatment with the inhibitors have no effect on anti-CD18 induced apoptosis.¶Conclusion: Anti-CD18 pansorbins induce apoptosis in neutrophils through an alternative pathway not involving reactive oxygen species and independent of tyrosine phosphorylation, cytoskeletal reorganisation and caspases.¶

Key words: Programmed cell death - Reactive oxygen species - Adhesion - Cytoskeleton - Tyrosine phosphorylation 

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Copyright information

© Birkhäuser Verlag, 2000

Authors and Affiliations

  • J. Larsson
    • 1
  • L. Serrander
    • 2
  • O. Stendahl
    • 2
  • H. Lundqvist-Gustafsson
    • 1
  1. 1.Division of Pathology II, Faculty of Health Sciences, Linköping University, 58185 Linköping, Sweden, Fax: +4613221529, e-mail: jenny.larsson@inr.liu.seSE
  2. 2.Division of Medical Microbiology, Faculty of Health Sciences, Linköping University, 58185 Linköping, SwedenSE

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