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Inflammation Research

, Volume 49, Issue 4, pp 170–176 | Cite as

Participation of endogenous endothelins in delayed eosinophil and neutrophil recruitment in mouse pleurisy

  • A. L. F. Sampaio
  • G. A. Rae
  • M. G. M. O. Henriques

Abstract.

Objective: Investigate the role of endothelins in leukocyte recruitment in allergic and non allergic inflammation.¶Methods: Pleurisy was induced in mice by intrathoracic injection of ovalbumin (OVA; in sensitized animals), E. coli LPS, carrageenan, Mycobacterium bovis (BCG) or zymosan. Animals were treated with BQ-123 or BQ-788 (1.5-150 pmol/cavity), or intravenously with bosentan (30 mg/kg).¶Results: None of the ET receptor antagonists modified early neutrophil recruitment (at 4h) induced by OVA, LPS, carrageenan, BCG or zymosan or plasma leakage caused by carrageenan or zymosan. Mononuclear and eosinophil accumulation triggered by OVA were reduced by BQ-123 (150 pmol/cavity) or bosentan (68 and 43% inhibition of eosinophilia), but unaffected by BQ-788. BQ-123 and bosentan also inhibited LPS increases in neutrophil (by 67 and 40%) and eosinophil (by 63 and 74%) at 24 h.¶Conclusions: Endothelins, acting via ETA receptors, play a role in late eosinophil and neutrophil accumulation (24h), but not in the acute (4h) neutrophilic response.

Key words: Endothelin — Eosinophil — Neutrophil — Allergy — LPS 

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Copyright information

© Birkhäuser Verlag, 2000

Authors and Affiliations

  • A. L. F. Sampaio
    • 1
  • G. A. Rae
    • 2
  • M. G. M. O. Henriques
    • 1
  1. 1.Laboratório de Farmacologia Aplicada, Instituto de Tecnologia em Fármacos - FARMANGUINHOS - Fundação Oswaldo Cruz, Rua Sizenando Nabuco 100, Manguinhos, Rio de Janeiro, 21041-250, Brasil, Fax: +5521 2802773, e-mail: henrique@gene.dbbm.fiocruz.brBR
  2. 2.Department of Pharmacology, CCB, Federal University of Santa Catarina, Florianopolis, Brazil BR

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