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Inflammation Research

, Volume 48, Issue 4, pp 224–228 | Cite as

A comparison of the inhibitory effects of budesonide, beclomethasone dipropionate, dexamethasone, hydrocortisone and tixocortol pivalate on cytokine release from leukocytes recovered from human bronchoalveolar lavage

  • C. J. Whelan
  • A. N. Payne
  • J.-M. Planquois
Article

Abstract

Objective: The potency of budesonide, beclomethasone dipropionate (BDP), dexamethasone, hydrocortisone and tixocortol pivalate as inhibitors of interleukin-5 (IL-5) and interferon-γ (IFNγ) release from human bronchoalveolar lavage cells in vitro were compared.¶Methods: BAL leukocytes were obtained from patients undergoing bronchoscopy for diagnostic purposes. BAL leukocytes were activated with PHA (10 μg/ml) and PMA (10 ng/ml) and cultured for 48 h in the presence or absence of glucocorticoids. Culture supernatants were assayed for cytokines by ELISA.¶Results: Budesonide (10-9 to 10-7 M) and BDP (10-8 to 10-6 M) were the most potent glucocorticoids tested. Dexamethasone (10-7 to 10-5 M) was less potent, and the maximum inhibitory effect of dexamethasone was less than that produced by than budesonide or BDP. Tixocortol pivalate (10-6 to 3 × 10-5 M) caused a concentration-related inhibition of IL-5 release but only the highest concentration tested inhibited the release of IFNγ. Hydrocortisone (10-4 M) inhibited IL-5 and IFNγ release.¶Conclusion: We conclude that, unlike the other glucocorticoids tested, tixocortol pivalate appeared to be a selective inhibitor of IL-5 release. Possible mechanisms for this selectivity are discussed.

Key words: Glucocorticoid — Tixocortol pivalate — Interleukin-5 — Interferon-γ— BAL leukocytes 

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Copyright information

© Birkhäuser Verlag 1999

Authors and Affiliations

  • C. J. Whelan
    • 1
  • A. N. Payne
    • 2
  • J.-M. Planquois
    • 2
  1. 1.Department of Biosciences, University of Hertfordshire, College Lane, Hatfield, Herts, AL10 9AB, UKUK
  2. 2.Institute de Recherche Jouveinal, 3-9 rue de la Loge, B.P. 100, F-94265 Fresnes Cedex, FranceFrance

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