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Inflammation Research

, Volume 46, Issue 9, pp 348–353 | Cite as

Glucosaminylmuramyl dipeptide (GMDP) modulates endothelial cell activities in vitro but has no effect on angiogenesis in vivo

  • C. G. Li
  • S. Kumar
  • P. W. Ledger
  • J. M. Ponting
  • M. Carette
  • E. Allan
Article

Abstract.

Objective and Design: The aim of the study was to evaluate the effects of GMDP on angiogenesis in vivo and as a modulator of human umbilical vein endothelial cell proliferation, cell surface antigen expression and cell adhesion in vitro.¶Materials: Human umbilical vein endothelial cells (HUVEC), fertilized white leghorn chicken eggs, antibodies against adhesion molecules and glucosaminylmuramyl dipeptide (GMDP).¶Treatment: GMDP [0.01–100 μg/ml] applied to cell cultures for 6–72 h and to the chick chorioallantoic membrane (CAM) for four days.¶Methods: Angiogenic activity of GMDP in vivo was assessed using the CAM assay; HUVEC proliferation was measured by tritiated thymidine incorporation and cell cycle studies; cell surface antigen expression by indirect immunofluorescence and flow cytometry; cell adhesion by quantification of [3H]-thymidine labeled leukocyte adherence to HUVEC monolayers. Statistical analysis was performed using one-way ANOVA and if necessary was followed by Duncan's multiple range test for variables.¶Results: GMDP induced [3H]-thymidine incorporation in a concentration- and time-dependent manner (p < 0.003) and significantly increased the proportion of cells in the S phase of the cell cycle (p < 0.03). It weakly augmented the expression of ICAM-1 and CD31 but not adhesion of leukocytes to HUVEC monolayers GMDP was not angiogenic in the CAM assay.¶Conclusions: GMDP can modulate endothelial cell activity without the induction of angiogenesis in vivo which may have implications for its use as a therapeutic agent.

Key words: Glucosaminylmuramyl dipeptide — Human umbilical vein endothelial cells — ICAM-1 — CD31 — Cell adhesion 

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Copyright information

© Birkhäuser Verlag, Basel 1997

Authors and Affiliations

  • C. G. Li
    • 1
  • S. Kumar
    • 1
  • P. W. Ledger
    • 2
  • J. M. Ponting
    • 1
  • M. Carette
    • 1
  • E. Allan
    • 3
  1. 1.Department of Pathology and Rheumatology, Medical School, University of Manchester, Manchester M13 9PT, UK, Fax +44 161 275 5289UK
  2. 2.Peptech Ltd., Dyer Street, Cirencester, Glos GL7 2PF, UKUK
  3. 3.Christie Hospital, Wilmslow Road, Manchester M20 9BX, UKUK

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