IL-33/s-ST2 ratio, systemic symptoms, and basophil activation in Pru p 3-sensitized allergic patients
Although IL-33/ST2 axis is involved in the development of allergic diseases, its contribution in food allergy is still unknown.
In this study, we assessed the serum levels of IL-33 and its s-ST2 receptor in 53 control patients (without allergic diseases), 47 peach (Pru p 3)-sensitized allergic patients (SAP), and in 68 non-Pru p 3-SAP. Basophil activation test (BAT) was used to assess the basophil activation due to allergen exposure before and after the addition of s-ST2 to the blood samples from 5 Pru p 3-SAP.
IL-33 levels in Pru p 3-SAP were higher than in non-Pru p 3-SAP and in normal controls. Lower s-ST2 levels were found in Pru p 3-SAP than in non-Pru p 3-SAP. IL-33/s-ST2 ratio was higher in Pru p 3-SAP than in both non-Pru p 3-SAP and controls. Higher IL-33/s-ST2 ratio was observed in Pru p 3-SAP with severe than in those with mild systemic symptoms. BAT analysis in Pru p 3-SAP showed a decrease in basophil activation due to Pru p 3 exposure after the addition of s-ST2 to the blood samples.
An imbalance in the baseline levels of IL-33/ST2 pathway is present in Pru p 3-SAP. The measurement of this pathway might be helpful to detect patients at a higher risk of developing severe systemic symptoms.
KeywordsIL-33 receptor sST2 Food allergy Non-specific lipid-transfer protein Peach allergy sIgE to Pru p 3 Anaphylaxis Basophil activation test Panallergens
Soluble IL-33 receptor
- Pru p 3-SAP
Peach lipid-transfer protein (LTP)-sensitized allergic patients
- Non-Pru p 3-SAP
Non-Pru p 3-sensitized allergic patients
Specific immunoglobuline E
Basophil activation test
Skin prick test
Oral allergic syndrome
Enzyme-linked immunosorbent assay
Non-specific lipid-transfer protein
IL-1 receptor or like 1 gene
This work was supported by the Italian National Research Council. The authors thank Dr Massimiliano Di Vita for his unconditional support.
Conflict of interest
No conflicts of interest or disclosures to be made by any of the authors.
Consent for publication
Availability of data and material
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