Inflammation Research

, Volume 67, Issue 8, pp 671–679 | Cite as

IL-33/s-ST2 ratio, systemic symptoms, and basophil activation in Pru p 3-sensitized allergic patients

  • Carina G. Uasuf
  • Caterina Di Sano
  • Sebastiano Gangemi
  • Giuseppe Albeggiani
  • Diego Cigna
  • Paola Dino
  • Ignazio Brusca
  • Mark Gjomarkaj
  • Elisabetta Pace
Original Research Paper



Although IL-33/ST2 axis is involved in the development of allergic diseases, its contribution in food allergy is still unknown.


In this study, we assessed the serum levels of IL-33 and its s-ST2 receptor in 53 control patients (without allergic diseases), 47 peach (Pru p 3)-sensitized allergic patients (SAP), and in 68 non-Pru p 3-SAP. Basophil activation test (BAT) was used to assess the basophil activation due to allergen exposure before and after the addition of s-ST2 to the blood samples from 5 Pru p 3-SAP.


IL-33 levels in Pru p 3-SAP were higher than in non-Pru p 3-SAP and in normal controls. Lower s-ST2 levels were found in Pru p 3-SAP than in non-Pru p 3-SAP. IL-33/s-ST2 ratio was higher in Pru p 3-SAP than in both non-Pru p 3-SAP and controls. Higher IL-33/s-ST2 ratio was observed in Pru p 3-SAP with severe than in those with mild systemic symptoms. BAT analysis in Pru p 3-SAP showed a decrease in basophil activation due to Pru p 3 exposure after the addition of s-ST2 to the blood samples.


An imbalance in the baseline levels of IL-33/ST2 pathway is present in Pru p 3-SAP. The measurement of this pathway might be helpful to detect patients at a higher risk of developing severe systemic symptoms.


IL-33 receptor sST2 Food allergy Non-specific lipid-transfer protein Peach allergy sIgE to Pru p 3 Anaphylaxis Basophil activation test Panallergens 



Interleukin 33


Soluble IL-33 receptor

Pru p 3-SAP

Peach lipid-transfer protein (LTP)-sensitized allergic patients

Non-Pru p 3-SAP

Non-Pru p 3-sensitized allergic patients


Specific immunoglobuline E


Basophil activation test


Skin prick test


Oral allergic syndrome


Enzyme-linked immunosorbent assay


Non-specific lipid-transfer protein


IL-1 receptor or like 1 gene



This work was supported by the Italian National Research Council. The authors thank Dr Massimiliano Di Vita for his unconditional support.


Not applicable.

Conflict of interest

No conflicts of interest or disclosures to be made by any of the authors.

Consent for publication

Not applicable.

Availability of data and material

Not applicable.


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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Allergy Diseases Center “Prof G. Bonsignore”Institute of Biomedicine and Molecular Immunology “A. Monroy”(IBIM), National Research Council (CNR)PalermoItaly
  2. 2.Department of Clinical and Experimental Medicine, School and Division of Allergy and Clinical ImmunologyUniversity of MessinaMessinaItaly
  3. 3.Clinical Pathology, Allergy UnitBuccheri La Ferla HospitalPalermoItaly

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