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IL-33/s-ST2 ratio, systemic symptoms, and basophil activation in Pru p 3-sensitized allergic patients

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Abstract

Background

Although IL-33/ST2 axis is involved in the development of allergic diseases, its contribution in food allergy is still unknown.

Methods

In this study, we assessed the serum levels of IL-33 and its s-ST2 receptor in 53 control patients (without allergic diseases), 47 peach (Pru p 3)-sensitized allergic patients (SAP), and in 68 non-Pru p 3-SAP. Basophil activation test (BAT) was used to assess the basophil activation due to allergen exposure before and after the addition of s-ST2 to the blood samples from 5 Pru p 3-SAP.

Results

IL-33 levels in Pru p 3-SAP were higher than in non-Pru p 3-SAP and in normal controls. Lower s-ST2 levels were found in Pru p 3-SAP than in non-Pru p 3-SAP. IL-33/s-ST2 ratio was higher in Pru p 3-SAP than in both non-Pru p 3-SAP and controls. Higher IL-33/s-ST2 ratio was observed in Pru p 3-SAP with severe than in those with mild systemic symptoms. BAT analysis in Pru p 3-SAP showed a decrease in basophil activation due to Pru p 3 exposure after the addition of s-ST2 to the blood samples.

Conclusions

An imbalance in the baseline levels of IL-33/ST2 pathway is present in Pru p 3-SAP. The measurement of this pathway might be helpful to detect patients at a higher risk of developing severe systemic symptoms.

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Abbreviations

IL-33:

Interleukin 33

s-ST2:

Soluble IL-33 receptor

Pru p 3-SAP:

Peach lipid-transfer protein (LTP)-sensitized allergic patients

Non-Pru p 3-SAP:

Non-Pru p 3-sensitized allergic patients

sIgE:

Specific immunoglobuline E

BAT:

Basophil activation test

SPT:

Skin prick test

OAS:

Oral allergic syndrome

ELISA:

Enzyme-linked immunosorbent assay

nsLTP:

Non-specific lipid-transfer protein

IL-1RL:

IL-1 receptor or like 1 gene

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Acknowledgements

This work was supported by the Italian National Research Council. The authors thank Dr Massimiliano Di Vita for his unconditional support.

Funding

Not applicable.

Author information

Author notes

  1. Giuseppe Albeggiani: Deseased.

Authors and Affiliations

  1. Allergy Diseases Center “Prof G. Bonsignore”, Institute of Biomedicine and Molecular Immunology “A. Monroy”(IBIM), National Research Council (CNR), Palermo, Italy

    Carina G. Uasuf, Caterina Di Sano, Giuseppe Albeggiani, Diego Cigna, Paola Dino, Mark Gjomarkaj & Elisabetta Pace

  2. Department of Clinical and Experimental Medicine, School and Division of Allergy and Clinical Immunology, University of Messina, Messina, Italy

    Sebastiano Gangemi

  3. Clinical Pathology, Allergy Unit, Buccheri La Ferla Hospital, Via Ugo La Malfa 153, 90146, Palermo, Italy

    Ignazio Brusca

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  1. Carina G. Uasuf
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  2. Caterina Di Sano
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  3. Sebastiano Gangemi
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Correspondence to Elisabetta Pace.

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Responsible Editor: Bernhard Gibbs.

The present study is dedicated to the memory of Giuseppe Albeggiani who suddenly died on November 7th, 2016.

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Uasuf, C.G., Sano, C.D., Gangemi, S. et al. IL-33/s-ST2 ratio, systemic symptoms, and basophil activation in Pru p 3-sensitized allergic patients. Inflamm. Res. 67, 671–679 (2018). https://doi.org/10.1007/s00011-018-1157-4

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  • DOI: https://doi.org/10.1007/s00011-018-1157-4

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