The host control of a clinical isolate strain of P. aeruginosa infection is independent of Nod-1 but depends on MyD88

  • Fabiane Sônego
  • Fernanda V. S. Castanheira
  • Catarina V. Horta
  • Alexandre Kanashiro
  • Paula G. Czaikoski
  • Dario S. Zamboni
  • José Carlos Alves-Filho
  • Fernando Q. Cunha
Original Research Paper

Abstract

Objective and design

The objective of this study was to investigate the role of Nod1 in the recruitment of neutrophils into the infection site and in the establishment of the inflammatory response elicited by a clinical isolate strain of P. aeruginosa in vivo, while comparing it to the well-established role of MyD88 in this process.

Subjects

Wild-type, Nod1−/− and MyD88−/− mice, all with a C57Bl/6 background.

Methods

Mice were intranasally infected with Pseudomonas aeruginosa DZ605. Bronchoalveolar lavage and blood were harvested 6 or 20 h post-infection for evaluating bacterial load, chemokine levels and neutrophil migration. Survival post-infection was also observed.

Results

We show here that wild-type and Nod1−/− mice induce similar lung chemokine levels, neutrophil recruitment, and bacterial load, thus leading to equal survival rates upon P. aeruginosa pulmonary infection. Furthermore, we confirmed the essential role of MyD88-dependent signalling in recruiting neutrophils and controlling P. aeruginosa-induced pulmonary infection.

Conclusion

The results suggest that in contrast to MyD88, under our experimental conditions, the absence of Nod1 does not impair the recruitment of neutrophils in response to P. aeruginosa DZ605.

Keywords

Nod1 MyD88 Pseudomonas aeruginosa Pneumonia Neutrophil migration Chemokines 

Notes

Acknowledgements

The authors would like to thank Agnes Afrodite S Albuquerque, Ieda Regina dos Santos, Ana Kátia dos Santos, Giuliana Bertozi and Marco Antônio Silva for their technical assistance. This work was supported by São Paulo Research Foundation (FAPESP), grants #2008/11593-4 and #2011/19670-0, CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and European Community’s Seventh Framework Programme [FP7-2007-2013] under grant agreement number HEALTH-F4-2011-281608. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Compliance with ethical standards

Conflict of interest

The authors declares that there is no conflicts of interest.

Supplementary material

11_2018_1135_MOESM1_ESM.doc (91 kb)
Supplementary material 1 (DOC 91 KB)

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2018

Authors and Affiliations

  • Fabiane Sônego
    • 1
  • Fernanda V. S. Castanheira
    • 1
  • Catarina V. Horta
    • 2
  • Alexandre Kanashiro
    • 1
  • Paula G. Czaikoski
    • 1
  • Dario S. Zamboni
    • 2
  • José Carlos Alves-Filho
    • 1
  • Fernando Q. Cunha
    • 1
  1. 1.Departamento de FarmacologiaFaculdade de Medicina de Ribeirão PretoRibeirão PretoBrazil
  2. 2.Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos, Faculdade de Medicina de Ribeirão PretoUniversidade de São PauloRibeirão PretoBrazil

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