Insulin-like growth factor-6 (IGFBP-6) stimulates neutrophil oxidative burst, degranulation and chemotaxis
The aim of this study was to understand whether insulin-like growth factor-binding protein-6 (IGFBP-6) has functional effects on neutrophils, in particular when they cross epithelium during inflammation. We found that IGFBP-6 increased ROS production (cytofluorimetry), degranulation of primary and tertiary granules (ELISA) and transmigration through the epithelial monolayer. No priming by IGFBP-6 on neutrophils stimulated by either PMA or fMLP was observed. IGFBP-6 is an agonist of neutrophils’ functions, most likely when these cells have been already activated by other stimuli.
KeywordsNeutrophils IGFBP-6 Oxidative burst Degranulation Chemotaxis
This work was in part funded by Associazione Italiana contro le Leucemie-Linfomi e Mieloma (Section of Foggia) (A. Liso). S. Castellani is a researcher funded by Intervento Cofinanziato dal Fondo di Sviluppo e Coesione 2007–2013—APQ Ricerca Regione Puglia “Programma regionale a sostegno della specializzazione intelligente e delle sostenibilità sociale ed ambientali—Future In Research”.
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