SERPING1 mRNA overexpression in monocytes from HIV+ patients
The HIV-1 virus activates the complement system, an essential element of the immune system. SERPING1 is a protease inhibitor that disables C1r/C1s in the C1 complex of the classical complement pathway.
In this paper, we performed an analysis of several microarrays deposited in GEO dataset to demonstrate that SERPING1 mRNA is modulated in CD14+ monocytes from HIV-1-infected individuals. In addition, data were validated on monocytes isolated from seronegative healthy volunteers, treated with IFNs.
Our analysis shows that SERPING1 mRNA is overexpressed in monocytes from HIV-1+ patients and the expression levels correlate positively with viral load and negatively with the CD4+ T-cell count. Of note, anti-retroviral therapy is able to reduce the levels of SERPING1 mRNA, ex vivo. In addition, we found that 30% of the SERPING1 genes network is upregulated in monocytes from HIV-1+ patients. Noteworthy, the expression levels of IFITM1—an antiviral molecule belonging to the genes network—correlate positively with SERPING1 expression. Interestingly, the monocytes treatment with IFN-gamma, IFN-beta and IFN-alpha significantly upregulates the SERPING1 mRNA expression levels.
From the outcome of our investigation, it is possible to conclude that SERPING1 and its network serve as important components of the innate immune system to restrict HIV-1 infection.
KeywordsSERPING1 C1-INH Monocyte Complement HIV-1 IFN-gamma
Low viral load
High viral load
Human immunodeficiency virus 1
Acute human immunodeficiency virus (HIV) infection
- C1-INH or SERPING1
50% Tissue culture infective dose
Interferon-induced transmembrane protein 1
Highly active anti-retroviral therapy
Complement component 4 fragment d
Type I and II hereditary angioedema
Plasma viral load
Human peripheral blood mononuclear cells
Blood viral load
We would also like to show our gratitude to the authors of microarray dataset (GSE18464, GSE5220, GSE13395 and GSE25669) made available on line, for consultation and re-analysis.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interests.
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