Association of interleukin-10 gene promoter polymorphism in spontaneous abortions: a family-based triad study
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The present study is a triad study designed to determine the co-relation of IL-10 −819C/T promoter polymorphism with the risk of spontaneous abortions.
A total of 50 families with spontaneous abortions and 60 families with medically terminated pregnancies were considered for the present study. Fetal tissue of less than 20 weeks of gestation along with peripheral blood from all the couples was collected in this study.
A standard amplification refractory mutation system-polymerase chain reaction was carried out to determine the IL 10 genotype in all the subjects. Odd’s ratio and their respective 95 % confidence intervals were used to determine the strength of association between IL-10 promoter gene polymorphism and spontaneous abortions.
The study revealed a statistically significant association of IL-10 −819C/T polymorphism between the two family groups among fetuses (p = 0.0000003) and mothers (p = 0.0000001). No significant difference was observed in the genotype distribution of IL-10 among fathers.
An increased frequency of TT genotype and T allele was observed in spontaneously aborted fetuses and their mothers compared to respective controls. In conclusion, IL-10 C −819T gene promoter polymorphism may act as a major genetic regulator in the etiology of spontaneous abortions.
KeywordsInterleukin-10 Polymorphism Fetus Spontaneous abortions Polymerase chain reaction
The authors acknowledge Indian Council of Medical Research (ICMR), New Delhi for providing Senior Research Fellowship to Mrs. M. Vidyadhari.
Compliance with ethical standards
Conflict of interest
Authors declare that they have no conflict of interest.
- 14.Qaddourah RH, Magdoud K, Saldanha FL, Mahmood N, Mustafa FE, Mahjoub T, Almawi WY. IL-10 gene promoter and intron polymorphisms and changes in IL-10 secretion in women with idiopathic recurrent miscarriage. Hum Reprod. 2014;1–10.Google Scholar
- 17.Sowmya S, Ramaiah A, Sunitha T, Nallari P, Jyothy A, Venkateshwari A. Role of IL-10 −819 (C/T) promoter polymorphism in pre eclampsia. Inflammation. 2014;37:1022–7.Google Scholar
- 20.Von Wollf M, Thaler CJ, Strowitzki T, Broome J, Stolz W, Tabibzadeh S. Regulated expression of cytokines in human endometrium throughout the menstrual cycle: dysregulation in habitual abortion. Mol Hum Reprod. 2000;6:227–34.Google Scholar
- 33.Radoi VE, Bohiltea CL, Bara C. Interleukin 10 gene polymorphisms and recurrent pregnancy loss in Romanian population. Hum Reprod. 2012;8:94–7.Google Scholar
- 34.Bohiltea CL, Radoi VE. Interleukin-6 and interleukin-10 gene polymorphisms and recurrent pregnancy loss in Romanian population. Iran J Reprod Med. 2014;12:617–22.Google Scholar