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Inflammation Research

, Volume 64, Issue 12, pp 963–969 | Cite as

Association of interleukin-10 gene promoter polymorphism in spontaneous abortions: a family-based triad study

  • M. Vidyadhari
  • M. Sujatha
  • P. Krupa
  • A. Jyothy
  • Pratibha Nallari
  • A. Venkateshwari
Original Research Paper

Abstract

Objective

The present study is a triad study designed to determine the co-relation of IL-10 −819C/T promoter polymorphism with the risk of spontaneous abortions.

Materials

A total of 50 families with spontaneous abortions and 60 families with medically terminated pregnancies were considered for the present study. Fetal tissue of less than 20 weeks of gestation along with peripheral blood from all the couples was collected in this study.

Methods

A standard amplification refractory mutation system-polymerase chain reaction was carried out to determine the IL 10 genotype in all the subjects. Odd’s ratio and their respective 95 % confidence intervals were used to determine the strength of association between IL-10 promoter gene polymorphism and spontaneous abortions.

Results

The study revealed a statistically significant association of IL-10 −819C/T polymorphism between the two family groups among fetuses (p = 0.0000003) and mothers (p = 0.0000001). No significant difference was observed in the genotype distribution of IL-10 among fathers.

Conclusion

An increased frequency of TT genotype and T allele was observed in spontaneously aborted fetuses and their mothers compared to respective controls. In conclusion, IL-10 C −819T gene promoter polymorphism may act as a major genetic regulator in the etiology of spontaneous abortions.

Keywords

Interleukin-10 Polymorphism Fetus Spontaneous abortions Polymerase chain reaction 

Notes

Acknowledgments

The authors acknowledge Indian Council of Medical Research (ICMR), New Delhi for providing Senior Research Fellowship to Mrs. M. Vidyadhari.

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

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Copyright information

© Springer Basel 2015

Authors and Affiliations

  • M. Vidyadhari
    • 1
  • M. Sujatha
    • 1
  • P. Krupa
    • 2
  • A. Jyothy
    • 1
  • Pratibha Nallari
    • 3
  • A. Venkateshwari
    • 1
  1. 1.Institute of Genetics and Hospital for Genetic DiseasesOsmania UniversityHyderabadIndia
  2. 2.Government Modern Maternity HospitalHyderabadIndia
  3. 3.Department of GeneticsOsmania UniversityHyderabadIndia

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