Decreased CD200R expression on monocyte-derived macrophages correlates with Th17/Treg imbalance and disease activity in rheumatoid arthritis patients
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CD200 is expressed on various cell types, including T cells, while the CD200 receptor (CD200R) is expressed on myeloid cells such as monocytes-derived macrophages (MDMs). The CD200–CD200R interaction has been shown to play an important role in the prevention of autoimmune disease. Thus, we hypothesized that CD200/CD200R1 is involved in the pathogenesis of rheumatoid arthritis (RA).
In total, 35 RA patients and 17 healthy controls (HCs) were enrolled in this study. CD200/CD200R1 expression and Th17/Treg were examined by flow cytometry. Serum levels of interleukin (IL)-2, interferon-γ (IFN-γ), IL-4 and IL-10 were detected by ELISA. Disease activity was evaluated according to the C-reactive protein (CRP) levels, erythrocyte sedimentation rates (ESR) and 28-joint disease activity score (DAS28) scores.
Compared with HCs, RA patients exhibited a significantly decreased level of CD200R1 on MDMs. CD200R1 expression correlated negatively with DAS28, ESR, and CRP levels. This abnormal expression was associated with Th17/Treg imbalance in the active RA patients. However, expression of CD200R1 was not correlated with Th1 (IL-2, IFN-γ) or Th2 (IL-4, IL-10) cytokine responses.
In this study, we demonstrate a significant correlation between CD200R1+ cells and disease severity in RA patients, thus indicating the relevance of the CD200/CD200R1 signaling pathway's potential involvement in the pathogenesis of RA.
KeywordsCD200R1 Macrophages Rheumatoid arthritis Regulatory T cells Th17 cells
This study was supported by grants from the Scientific Research Foundation of Zhejiang Provincial Education Department (Y201121334) (Y201226006), Medical Science and Technology Project of Zhejiang Province (2012KYB088), and Science and Technology Project of Zhejiang Province Department of Science and Technology (2012F82G2010026) (2013C33G2010397).
Conflict of interest
- 20.Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, Cooper NS, et al. The American Rheumatology Association revised criteria for the classification of rheumatoid arthritis. Arthr Rheum. 1987;1988(31):315–24.Google Scholar
- 22.Baran J, Kowalczyk D, Ozóg M, Zembala M. Three-color flow cytometery detection of intracellular cytokine in peripheral blood mononuclear cells: comparative analysis of phorbol myristate acetate-ionomycin and phytohemagglutinin stimulation. Clin Diagn Lab Immunol. 2001;8:303–13.PubMedPubMedCentralGoogle Scholar
- 28.Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwazenberger P, Coenen-de Roo CJ, Kolls JK. IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NK-κB ligand/osteoprotegerin balance. J Immunol. 2003;170:2655–62.PubMedCrossRefGoogle Scholar