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Decreased CD200R expression on monocyte-derived macrophages correlates with Th17/Treg imbalance and disease activity in rheumatoid arthritis patients

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Abstract

Objectives

CD200 is expressed on various cell types, including T cells, while the CD200 receptor (CD200R) is expressed on myeloid cells such as monocytes-derived macrophages (MDMs). The CD200–CD200R interaction has been shown to play an important role in the prevention of autoimmune disease. Thus, we hypothesized that CD200/CD200R1 is involved in the pathogenesis of rheumatoid arthritis (RA).

Methods

In total, 35 RA patients and 17 healthy controls (HCs) were enrolled in this study. CD200/CD200R1 expression and Th17/Treg were examined by flow cytometry. Serum levels of interleukin (IL)-2, interferon-γ (IFN-γ), IL-4 and IL-10 were detected by ELISA. Disease activity was evaluated according to the C-reactive protein (CRP) levels, erythrocyte sedimentation rates (ESR) and 28-joint disease activity score (DAS28) scores.

Results

Compared with HCs, RA patients exhibited a significantly decreased level of CD200R1 on MDMs. CD200R1 expression correlated negatively with DAS28, ESR, and CRP levels. This abnormal expression was associated with Th17/Treg imbalance in the active RA patients. However, expression of CD200R1 was not correlated with Th1 (IL-2, IFN-γ) or Th2 (IL-4, IL-10) cytokine responses.

Conclusion

In this study, we demonstrate a significant correlation between CD200R1+ cells and disease severity in RA patients, thus indicating the relevance of the CD200/CD200R1 signaling pathway's potential involvement in the pathogenesis of RA.

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Acknowledgments

This study was supported by grants from the Scientific Research Foundation of Zhejiang Provincial Education Department (Y201121334) (Y201226006), Medical Science and Technology Project of Zhejiang Province (2012KYB088), and Science and Technology Project of Zhejiang Province Department of Science and Technology (2012F82G2010026) (2013C33G2010397).

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Department of Clinical Laboratory, The Second Affiliated Hospital of School of Medicine, Zhejiang University, Jie Fang Road 88#, Hangzhou, 310009, People’s Republic of China

    Song Gao & Xu Feng Yang

  2. Key Laboratory of Combined Multi-Organ Transplantation, Ministry of Public Health, First Affiliated Hospital, Zhejiang University School of Medicine, Qing Chun Road 79#, Hangzhou, 310003, People’s Republic of China

    Bing Hao

  3. Department of Rheumatology, First Affiliated Hospital, Zhejiang University School of Medicine, Qing Chun Road 79#, Hangzhou, 310003, People’s Republic of China

    Wei Qian Chen

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  1. Song Gao
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  2. Bing Hao
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Corresponding author

Correspondence to Bing Hao.

Additional information

Responsible Editor: Liwu Li.

S. Gao and B. Hao contributed equally to this work and should be considered co-first authors.

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Gao, S., Hao, B., Yang, X.F. et al. Decreased CD200R expression on monocyte-derived macrophages correlates with Th17/Treg imbalance and disease activity in rheumatoid arthritis patients. Inflamm. Res. 63, 441–450 (2014). https://doi.org/10.1007/s00011-014-0716-6

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  • DOI: https://doi.org/10.1007/s00011-014-0716-6

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