Inflammation Research

, Volume 62, Issue 11, pp 951–959 | Cite as

Suppression of Th1 cytokine production by a peptide derived from C4b

  • Yuji Takeda
  • Kenta Kaneda
  • Fumie Jimma
  • Noriyuki Shiobara
  • Abbi R. Saniabadi
  • Ichiro Wakabayashi
Original Research Paper



The complement system has been proposed to play a significant role in the regulation of T-cell responses. However, the precise mechanism underlying C4-induced immune tolerance remains to be clarified. We recently reported that monomeric C4b inhibits CXCL10 production from blood cells. The purpose of this study was to verify the active site of monomeric C4b.

Materials and methods

We investigated the in vitro effects of a C4b-derived peptide (VPAGSARPVAFSVVPTAAA), named HP2 (highly homologous peptide 2), on the IFN-β-induced production of CXCL10 in human blood and the in vivo effects of the administration of HP2 on Th1/2 cytokine production in the spleen in mice. We also tested whether the administration of HP2 influences symptoms of experimentally induced ulcerative colitis in mice.


HP2 inhibited CXCL10 production in human blood, and the administration of HP2 significantly suppressed the production of Th1 cytokines, such as IL-2, IFN-γ, and TNF-α, in spleen cells isolated from mice. The administration of HP2 in the mice significantly improved the symptoms of colitis, with down-regulation of colitogenic CD4+CD45RBhigh T cells and up-regulation of CD4+LAP/TGF-β1+ T cells.


The amino acid sequence described above is suggested to be the active site in C4b for the inhibition of Th1 cytokine production. These results should contribute to the development of new drugs suppressing autoimmune responses.


Complement Chemokine Inflammatory disease Peptide Th1 cytokine 



The authors would like to thank Mr. Hiromu Shibusawa for his valuable technical support.


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Copyright information

© Springer Basel 2013

Authors and Affiliations

  • Yuji Takeda
    • 1
  • Kenta Kaneda
    • 2
  • Fumie Jimma
    • 2
  • Noriyuki Shiobara
    • 2
  • Abbi R. Saniabadi
    • 2
  • Ichiro Wakabayashi
    • 1
  1. 1.Department of Environmental and Preventive MedicineHyogo College of MedicineNishinomiyaJapan
  2. 2.JIMRO Institute, Immunoresearch DivisionTakasakiJapan

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