Inflammation Research

, Volume 62, Issue 3, pp 313–324 | Cite as

Polysaccharides of Dendrobium officinale inhibit TNF-α-induced apoptosis in A-253 cell line

  • L. Xiang
  • C. W. Stephen Sze
  • T. B. Ng
  • Y. Tong
  • P. C. Shaw
  • C. W. Sydney Tang
  • Y. B. Kalin Zhang
Original Research Paper



Our previous study demonstrated that polysaccharides of Dendrobium officinale Kimura et Migo (DP) were capable of enhancing immunomodulation in an experimental model of Sjögren’s syndrome, a chronic autoimmune disease mainly affecting the salivary glands. In the present study, we further investigated the protective effect of DP on a human salivary gland cell line A-253 against tumor necrosis factor (TNF)-α-induced apoptosis.


TNF-α (100 U/ml) was used as the stimulus for treating the A-253 cells to induce cellular apoptosis. Nuclear factor-kappa B (NF-κB, p65), phosphorylation of mitogen-activated protein kinases (MAPK), reactive oxygen species (ROS) generation, mitochondrial membrane potential and proapoptotic proteins were examined. A-253 cells were pre-treated with DP for 12 h before TNF-α stimulation.


We observed translocation of NF-κB into the nuclei, prolonged MAPK, excessive ROS generation and strongly decreased mitochondrial membrane potential, and subsequently cytochrome C release and caspase-3 activation. However, pre-treatment with DP significantly inhibited the TNF-α-induced apoptotic factors.


Our data suggested the inhibitory effect of DP on TNF-α-induced apoptosis in a human salivary gland cell line. This inhibition indicated potential inference of DP in the initial plasma membrane-bound complex of TNF-α and its receptors.


TNF-α A-253 Polysaccharides Dendrobium officinale Apoptosis 



This work was supported by grants (Project No. 20730472 and Project No. 201011159206) from the University of Hong Kong.


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Copyright information

© Springer Basel 2012

Authors and Affiliations

  • L. Xiang
    • 1
  • C. W. Stephen Sze
    • 1
  • T. B. Ng
    • 2
  • Y. Tong
    • 1
  • P. C. Shaw
    • 3
  • C. W. Sydney Tang
    • 4
  • Y. B. Kalin Zhang
    • 1
  1. 1.School of Chinese MedicineThe University of Hong KongHong KongChina
  2. 2.School of Biomedicine ScienceHong KongChina
  3. 3.School of Life ScienceHong KongChina
  4. 4.Department of Medicine, Faculty of MedicineThe University of Hong KongHong KongChina

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