GC/MS-based profiling of amino acids and TCA cycle-related molecules in ulcerative colitis
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The roles that amino acids play in immunity and inflammation are well defined, and the relationship between inflammatory bowel disease (IBD) and certain amino acids has recently attracted attention. In this study, the levels of amino acids and trichloroacetic acid (TCA) cycle-related molecules in the colonic tissues and sera of patients with ulcerative colitis (UC) were profiled by gas chromatography/mass spectrometry (GC/MS), with the aim of evaluating whether the clinical state induced by UC leads to variations in the amino acid profile.
Materials and methods
Colonic biopsy samples from 22 UC patients were used, as well as serum samples from UC patients (n = 13), Crohn’s disease (CD) patients (n = 21), and healthy volunteers (n = 17).
In the GC/MS-based profiling of amino acids and TCA cycle-related molecules, lower levels of 16 amino acids and 5 TCA cycle-related molecules were observed in the colonic lesion tissues of the UC patients, and the serum profiles of amino acids and TCA cycle-related molecules of the UC patients were different from those of the CD patients and healthy volunteers.
Our study raises the possibility that GC/MS-based profiling of amino acids and TCA cycle-related molecules is a useful early diagnostic tool for UC.
KeywordsUlcerative colitis Amino acid Metabolomics GC/MS
Inflammatory bowel disease
Nuclear magnetic resonance analysis
Gas chromatography/mass spectrometry
Liquid chromatography/mass spectrometry
Capillary electrophoresis/mass spectrometry
Clinical activity index
International Organization for the Study of Inflammatory Bowel Disease
Principal component analysis
Partial least squares discriminant analysis
Grant support: T.Y., T.A., and M.Y. were supported by a grant from the Global COE Program “Global Center of Excellence for Education and Research on Signal Transduction Medicine in the Coming Generation” from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. S.N. and T.A. were partially supported by a grant from the “Young researchers training program for promoting innovation” program of the Special Coordination Fund for Promoting Science and Technology from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. Y.S. was partially supported by a grant from the Education Program for Specialized Clinicians in the Support Program for Improving Graduate School Education from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. We thank Kazuko Nagase (The Divisions of Lower Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine) for sample collection and preparation.
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