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Soluble vascular endothelial growth factor (VEGF) receptor-1 inhibits migration of human monocytic THP-1 cells in response to VEGF

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Abstract

Objective

We aimed to investigate the regulation and contribution of vascular endothelial growth factor (VEGF) and sFlt-1(1–3) to human monocytic THP-1 migration.

Materials and methods

Ad-sFlt-1/FLAG, a recombinant adenovirus carrying the human sFlt-1(1–3) (the first three extracellular domains of FLT-1, the hVEGF receptor-1) gene, was constructed. L929 cells were infected with Ad-sFlt-1/FLAG and the expression of sFlt-1 was detected by immunofluorescent assay and ELISA. Corning® Transwell® Filter Inserts containing polyethylene terephthalate (PET) membranes with pore sizes of 3 μm were used as an experimental model to simulate THP-1 migration. Five VEGF concentrations (0, 0.1, 1, 10 and 100 ng/ml), four concentrations of sFlt-1(1–3)/FLAG expression supernatants (0.1, 1, 10 and 100 ng/ml), and monocyte chemoattractant protein-1 (MCP-1, 10 ng/ml) were used to test the ability of THP-1 cells to migrate through PET membranes.

Results

The sFlt-1(1–3) gene was successfully recombined into Ad-sFlt-1/FLAG. sFlt-1(1–3) was expressed in L929 cells transfected with Ad-sFlt-1/FLAG. THP-1 cell migration increased with increasing concentrations of VEGF, while cell migration decreased with increasing concentrations of sFlt1(1–3)/FLAG. sFlt1(1–3)/FLAG had no effect on MCP-1-induced cell migration.

Conclusions

This study demonstrated that VEGF is able to elicit a migratory response in THP-1 cells, and that sFlt-1(1–3) is an effective inhibitor of THP-1 migration towards VEGF.

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Authors and Affiliations

  1. Department of Neurology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People’s Republic of China

    Cansheng Zhu, Xiaohong Chen, Zhengqi Lu, Guoyu Zhou, Dunjing Wang, Jian Bao & Xueqiang Hu

  2. Department of Cardiovascular diseases, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, People’s Republic of China

    Zhaojun Xiong

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  1. Cansheng Zhu
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  2. Zhaojun Xiong
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  3. Xiaohong Chen
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  4. Zhengqi Lu
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  5. Guoyu Zhou
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  6. Dunjing Wang
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  7. Jian Bao
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  8. Xueqiang Hu
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Correspondence to Xueqiang Hu.

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Responsible editor: Graham Wallace.

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Zhu, C., Xiong, Z., Chen, X. et al. Soluble vascular endothelial growth factor (VEGF) receptor-1 inhibits migration of human monocytic THP-1 cells in response to VEGF. Inflamm. Res. 60, 769–774 (2011). https://doi.org/10.1007/s00011-011-0332-7

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  • DOI: https://doi.org/10.1007/s00011-011-0332-7

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