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β-1,4-Galactosyltransferase-involved in lipopolysaccharide-induced adhesion of schwann cells

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Abstract

Objective and design

The carbohydrate moieties of glycoprotein are associated with some inflammatory diseases by affecting a wide range of biological functions of cells. This study aimed to investigate the role of β-1,4-galactosyltransferase-I (β-1,4-GalT-I) in adhesion of Schwann cells during inflammation.

Subjects

A rat Schwann cell line, RSC 96 was used.

Methods

We used western blotting to detect the expression of β-1,4-GalT-I. Flow cytomety was used to measure the galactosylation of glycoproteins on cell surfaces. Immunofluorescent staining was used to examine the expression of α6 integrin, focal adhesion kinase (FAK) and F-actin. Tyrosine phosphorylation of FAK was detected by immunoprecipitation. An adhesion assay was performed to investigate the adhesion of Schwann cells. One-way ANOVA was used to compare differences between the operated and the control group.

Results

Schwann cell adhesion was induced by LPS stimulation and was accompanied by upregulation of β-1,4-GalT-I expression and galactosylation of glycoproteins. There was a change of localization of FAK and cytoskeleton organization in LPS treated cells compared with control cells. The pretreated cells enhanced tyrosine phosphorylation of FAK compared with control cells in the adhesion process. With the increased cell surface expression of α6 integrin and β-1,4-GalT-I, the adhesion of Schwann cells on laminin was increased as well.

Conclusions

These results suggested that β-1,4-GalT-I may play an important role in adhesion of Schwann cells during inflammation.

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References

  1. Moalem G, Tracey DJ. Immune and inflammatory mechanisms in neuropathic pain. Brain Res Rev. 2006;51:240–64.

    Article  CAS  PubMed  Google Scholar 

  2. Liu J, Chau CH, Liu H, Jang BR, Li X, Chan YS. Upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth. J Cell Sci. 2006;119:933–42.

    Article  CAS  PubMed  Google Scholar 

  3. Frei R, Dowling J, Carenini S, Fuchs E, Martini R. Myelin formation by Schwann cells in the absence of beta4 integrin. Glia. 1999;27:269–74.

    Article  CAS  PubMed  Google Scholar 

  4. Martini R. Expression and functional roles of neural cell surface molecules and extracellular matrix components during development and regeneration of peripheral nerves. J Neurocytol. 1994;23:1–28.

    Article  CAS  PubMed  Google Scholar 

  5. Miller SJ, Li H, Rizvi TA, Huang Y, Johansson G, Bowersock J. Brain lipid binding protein in axon-Schwann cell interactions and peripheral nerve tumorigenesis. Mol Cell Biol. 2003;23:2213–24.

    Article  CAS  PubMed  Google Scholar 

  6. Shen A, Chen J, Qian J, Zhu J, Hu L, Yan M. Elevated beta1, 4-galactosyltransferase-I induced by the intraspinal injection of lipopolysaccharide. Glycoconj J. 2009;26:19–31.

    Article  CAS  PubMed  Google Scholar 

  7. Abbott KL, Matthews RT, Pierce M. Receptor tyrosine phosphatase beta (RPTPbeta) activity and signaling are attenuated by glycosylation and subsequent cell surface galectin-1 binding. J Biol Chem. 2008;283:33026–35.

    Article  CAS  PubMed  Google Scholar 

  8. Davies D. Temporal and spatial regulation of alpha6 integrin expression during the development of the cochlear-vestibular ganglion. J Comp Neurol. 2007;502:673–82.

    Article  CAS  PubMed  Google Scholar 

  9. Furukawa K, Sato T. Beta-1, 4-galactosylation of N-glycans is a complex process. Biochim Biophys Acta. 1999;1473:54–66.

    CAS  PubMed  Google Scholar 

  10. Hathaway HJ, Evans SC, Dubois DH, Foote CI, Elder BH, Shur BD. Mutational analysis of the cytoplasmic domain of beta1, 4-galactosyltransferase I: influence of phosphorylation on cell surface expression. J Cell Sci. 2003;116:4319–30.

    Article  CAS  PubMed  Google Scholar 

  11. Mori R, Kondo T, Nishie T, Ohshima T, Asano M. Impairment of skin wound healing in beta-1, 4-galactosyltransferase-deficient mice with reduced leukocyte recruitment. Am J Pathol. 2004;164:1303–14.

    CAS  PubMed  Google Scholar 

  12. Monick MM, Powers L, Butler N, Yarovinsky T, Hunninghake GW. Interaction of matrix with integrin receptors is required for optimal LPS-induced MAP kinase activation. Am J Physiol Lung Cell Mol Physiol. 2002;283:390–402.

    Google Scholar 

  13. Wassler MJ, Shur BD. Clustering of cell surface (beta)1, 4-galactosyltransferase I induces transient tyrosine phosphorylation of focal adhesion kinase and loss of stress fibers. J Cell Sci. 2000;113(Pt 2):237–45.

    CAS  PubMed  Google Scholar 

  14. Cheng C, Qin Y, Shao X, Wang H, Gao Y, Cheng M, Shen A. Induction of TNF-alpha by LPS in Schwann cell is regulated by MAPK activation signals. Cell Mol Neurobiol. 2007;27:909–21.

    Article  CAS  PubMed  Google Scholar 

  15. Yan M, Cheng C, Shao X, Qian J, Shen A, Xia C. Expression change of beta-1, 4 galactosyltransferase I, V mRNAs and Galbeta1, 4GlcNAc group in rat sciatic nerve after crush. J Mol Histol. 2008;39:317–28.

    Article  CAS  PubMed  Google Scholar 

  16. Yang H, Hu L, Chen J, Zhu J, Tao T, Zhang F. Lipopolysaccharide induced upregulation of beta-1,4-galactosyltransferase-I in schwann cell. Inflammation. 2009;. doi:10.1007/s10753-009-9131-5.

    Google Scholar 

  17. Glasgow LR, Paulson JC, Hill RL. Systematic purification of five glycosidases from Streptococcus (Diplococcus) pneumoniae. J Biol Chem. 1977;252:8615–23.

    CAS  PubMed  Google Scholar 

  18. Calderwood DA. Integrin activation. J Cell Sci. 2004;117:657–66.

    Article  CAS  PubMed  Google Scholar 

  19. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992;69:11–25.

    Article  CAS  PubMed  Google Scholar 

  20. Runyan RB, Maxwell GD, Shur BD. Evidence for a novel enzymatic mechanism of neural crest cell migration on extracellular glycoconjugate matrices. J Cell Biol. 1986;102:432–41.

    Article  CAS  PubMed  Google Scholar 

  21. Detrait E, Laduron S, Meremans V, Baron-Van Evercooren A, de Aguilar P, Knoops B. Expression of integrins by murine MSC80 Schwann cell line: relationship to cell adhesion and migration. Neurosci Lett. 1999;267:49–52.

    Article  CAS  PubMed  Google Scholar 

  22. Feltri ML, Graus Porta D, Previtali SC, Nodari A, Migliavacca B, Cassetti A. Conditional disruption of beta 1 integrin in Schwann cells impedes interactions with axons. J Cell Biol. 2002;156:199–209.

    Article  CAS  PubMed  Google Scholar 

  23. Roman J, Ritzenthaler JD, Boles B, Lois M, Roser-Page S. Lipopolysaccharide induces expression of fibronectin alpha 5 beta 1-integrin receptors in human monocytic cells in a protein kinase C-dependent fashion. Am J Physiol Lung Cell Mol Physiol. 2004;287:L239–49.

    Article  CAS  PubMed  Google Scholar 

  24. Isaji T, Sato Y, Zhao Y, Miyoshi E, Wada Y, Taniguchi N. N-glycosylation of the beta-propeller domain of the integrin alpha5 subunit is essential for alpha5beta1 heterodimerization, expression on the cell surface, and its biological function. J Biol Chem. 2006;281:33258–67.

    Article  CAS  PubMed  Google Scholar 

  25. Schaller MD, Borgman CA, Cobb BS, Vines RR, Reynolds AB, Parsons JT. pp125FAK a structurally distinctive protein-tyrosine kinase associated with focal adhesions. Proc Natl Acad Sci USA. 1992;89:5192–6.

    Article  CAS  PubMed  Google Scholar 

  26. Milner R, Wilby M, Nishimura S, Boylen K, Edwards G, Fawcett J. Division of labor of Schwann cell integrins during migration on peripheral nerve extracellular matrix ligands. Dev Biol. 1997;185:215–28.

    Article  CAS  PubMed  Google Scholar 

  27. Chen ZL, Strickland S. Laminin gamma1 is critical for Schwann cell differentiation, axon myelination, and regeneration in the peripheral nerve. J Cell Biol. 2003;163:889–99.

    Article  CAS  PubMed  Google Scholar 

  28. Grove M, Komiyama NH, Nave KA, Grant SG, Sherman DL, Brophy PJ. FAK is required for axonal sorting by Schwann cells. J Cell Biol. 2007;176:277–82.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30770488, No. 30870320, No. 31070723, and No. 81070275); Natural Science Foundation of Jiangsu province (No. BK2009156, No. BK2009157, No. BK2009161 and No. BK2010169).

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Authors and Affiliations

  1. Department of Microbiology and Immunology, Medical college Nantong University, 19 Qiqiu Road, Nantong, 226001, Jiangsu, People’s Republic of China

    Ling Hu, Jianping Chen, Zhiyun Ben, Xingxin He, Fupeng Zhang, Tao Tao & Chun Cheng

  2. Department of Orthopaedics, Affiliated Jiangyin Renmin Hospital of Nantong University, Key Laboratory for Neuroregeneration of JiangSu Province, Nantong University, Nantong, Jiangsu, People’s Republic of China

    Huiguang Yang, Xiaohong Li & Aiguo Shen

  3. Department of Clinical Laboratory, Shengzhou Center for Disease Control and Prevention, Shaoxing, Zhejiang, People’s Republic of China

    Ling Hu

Authors
  1. Ling Hu
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  2. Huiguang Yang
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  9. Chun Cheng
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Corresponding authors

Correspondence to Chun Cheng or Aiguo Shen.

Additional information

Responsible Editor: Graham Wallace.

L. Hu and H. Yang contributed equally to this work

Electronic supplementary material

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11_2010_251_MOESM1_ESM.tif

The expression of FAK was not upregulated by LPS. Schwann cells treated by 1 μg/ml LPS for 6 h, western blotting analysis showed there was no upregulation of FAK compared with normal one. (tif 1.33 Mb)

11_2010_251_MOESM2_ESM.tif

Galactosylation of integrin α6 expressed on Schwann cells. Integrin α6 was immunoprecipitated and then blotted by RCA-I. “No Ab” means no integrin α6 was added during immunoprecipitation but beads alone. (tif 578 kb)

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Hu, L., Yang, H., Chen, J. et al. β-1,4-Galactosyltransferase-involved in lipopolysaccharide-induced adhesion of schwann cells. Inflamm. Res. 60, 169–174 (2011). https://doi.org/10.1007/s00011-010-0251-z

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  • DOI: https://doi.org/10.1007/s00011-010-0251-z

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