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Inflammation Research

, Volume 58, Issue 6, pp 321–328 | Cite as

Effects of platelet release products on neutrophilic activity in human whole blood

  • B. Wu
  • G. Liu
  • K. Yube
  • M. Ueno
  • S. Tanaka
  • M. Onodera
  • Z. Jin
  • H. Sakamoto
Article

Abstract.

Objectives:

To investigate the effects of Platelet release products (PRPr) and their phagocytosis activators including ATP, ADP, and macromolecular activators of phagocytosis (MAPPs) on phagocytosis and oxidative burst activity by neutrophils in human whole blood.

Methods:

A whole blood-based flow cytometric assay was used to assess neutrophilic activity. Escherichia coli were used as the target organisms.

Results:

Phagocytosis and oxidative burst by neutrophils were markedly increased after treatment with PRPr or MAPPs  + ATP + ADP. The phagocytosis activation was more prominent in the first minute, and displayed a maximum enhancement of 4-fold. The maximum augmentation of oxidative burst was 5-fold which occurred at 5 mins. A striking finding was that the effect of MAPPs was evident from 5 mins onwards and increased with further incubation in both the phagocytosis and oxidative burst assays.

Conclusion:

These data suggest that the neutrophilic activity enhanced by PRPr depends more on ATP and ADP during the early phase and on MAPPs during the later phase of the phagocytic process. The present study reveals an important role for PRPr and their phagocytosis activators in the enhancement of neutrophilic activity in human whole blood.

Keywords.

Platelet release products Phagocytosis Oxidative burst Macromolecular activators of phagocytosis from platelet Human whole blood 

Abbreviations:

PRPr

platelet release products

MAPPs

macromolecular activators of phagocytosis from platelet

ATP

adenosine triphosphate

ADP

adenosine diphosphate

PBS

phosphate buffered saline

BSA

bovine serum albumin

RPMI-BSA

RPMI 1640 medium supplemented with 1% BSA

ROI

reactive oxygen intermediates

E. coli

Escherichia coli

FITC

Fluorescein isothiocyanate

DHR123

dihydrorhodamine 123

PI

propidium iodide

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Copyright information

© Birkhäuser Verlag, Basel 2009

Authors and Affiliations

  • B. Wu
    • 1
    • 4
  • G. Liu
    • 2
  • K. Yube
    • 3
  • M. Ueno
    • 1
  • S. Tanaka
    • 1
  • M. Onodera
    • 1
  • Z. Jin
    • 4
  • H. Sakamoto
    • 1
    • 5
  1. 1.Department of Inflammation Pathology, Faculty of MedicineKagawa UniversityKagawaJapan
  2. 2.Department of Pharmacology, Faculty of MedicineKagawa UniversityKagawaJapan
  3. 3.Life Science Research CenterKagawa UniversityKagawaJapan
  4. 4.Department of gynecology and obstetricsSecond Affiliated Hospital of China Medical UniversityShenyangChina
  5. 5.Department of Inflammation PathologyKagawa Medical UniversityKagawaJapan

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