Inflammation Research

, Volume 59, Supplement 2, pp 209–211 | Cite as

Involvement of histamine and histamine H2 receptors in nicotinamide-induced differentiation of human amniotic epithelial cells into insulin-producing cells

  • D. Szukiewicz
  • G. Szewczyk
  • T. K. Mittal
  • W. Rongies
  • S. Maslinski
Short Communication


Objective and design

Human amniotic epithelial cells (HAEC) resemble stem cells in their ability to differentiate into all three germ layers: endoderm, mesoderm, and ectoderm. Histamine receptors are expressed on HAEC. We examined the influence of histamine, and H1 and H2 antagonists on the generation of pancreatic islet beta-like cells from HAEC.

Materials and methods

HAEC were isolated after term pregnancies (N = 12) and cultured for 14 days with nicotinamide (10 mM) in normoxia. Altogether, 72 cultures were established. Histamine (100 μM) effects were investigated with mepyramine (10 μM) or cimetidine (10 μM). After 7 and 14 days, the mean concentration of C-peptide (MCCP) in the culture medium was measured immunoenzymatically as a marker of pancreatic differentiation.


MCCP was approximately threefold higher on day 14, compared to day 7. Histamine significantly increased MCCP, and more evident differences were observed after 7 days of culture than after 14 days. The mean percent increase ±SEM in MCCP amounted to 142.19 ± 21.7 and 79.03 ± 12.35 compared to the controls on day 7 and 14, respectively. H2 blockade significantly reduced histamine-related increase in MCCP, both on day 7 and 14 by 88.7 ± 14.3 and 39.2 ± 12.4%, respectively. H1 receptor antagonist did not affect MCPP.


Nicotinamide-induced pancreatic differentiation of HAEC into beta-like cells may be augmented, probably at its earlier stage, by histamine acting via H2 receptors.


Histamine Histamine H2 receptor Amniotic epithelial cells Pancreatic differentiation 


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Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  • D. Szukiewicz
    • 1
    • 2
  • G. Szewczyk
    • 1
  • T. K. Mittal
    • 2
  • W. Rongies
    • 3
  • S. Maslinski
    • 1
  1. 1.Department of General and Experimental PathologyMedical University of WarsawWarsawPoland
  2. 2.First Department of Obstetrics and Gynecology, Second Faculty of MedicineMedical University of WarsawWarsawPoland
  3. 3.Department of RehabilitationMedical University of WarsawWarsawPoland

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