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Inflammation Research

, Volume 59, Supplement 2, pp 205–207 | Cite as

Decrease in expression of histamine H2 receptors by human amniotic epithelial cells during differentiation into pancreatic beta-like cells

  • D. Szukiewicz
  • M. Pyzlak
  • A. Stangret
  • W. Rongies
  • D. Maslinska
Short Communication

Abstract

Objective and design

Considering the role of histaminergic pathway in the differentiation of stem cells, we compared expression patterns of H1 and H2 receptors in the human amniotic epithelial cells (HAEC) culture at different stages of nicotinamide-induced differentiation into PBLC with the control HAEC.

Material and methods

HAEC isolated after term pregnancies (N = 12) were cultured in vitro. Altogether, 72 cultures were established. The culture medium in the studied group was supplemented on Day 5 with nicotinamide (10 mM). C-peptide concentration in the medium collected every 3 days for 15 days was determined immunoenzymatically as a marker of differentiation. At the same intervals the cultures were formalin-fixed and paraffinembedded for H1 and H2 receptors immunostaining. Quantitative immunohistochemistry was applied for evaluation of H1 and H2 expression.

Results

C-peptide was detected on Day 6 and the levels were kept gradually increased until Day 12, then stayed at almost the same level, 3.7-fold higher than initially. Expression of H2 was unchanged until Day 9 after nicotinamide addition, then was significantly (p < 0.05) decreased and amounted (mean % value for the measurements performed on Day 12 and Day 15, ±SEM) 49.73 ± 11.03 of the reference value obtained in control HAEC.

Conclusion

Variable expression of H2 during nicotinamide-induced differentiation of HAEC into PBLC may define a time-point, indicating involvement of histamine at the earlier stages.

Keywords

Histamine receptors Amniotic epithelial cells Pancreatic differentiation 

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Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  • D. Szukiewicz
    • 1
    • 2
  • M. Pyzlak
    • 1
  • A. Stangret
    • 1
  • W. Rongies
    • 3
  • D. Maslinska
    • 1
  1. 1.Department of General and Experimental PathologyMedical University of WarsawWarsawPoland
  2. 2.First Department of Obstetrics and Gynecology, Second Faculty of MedicineMedical University of WarsawWarsawPoland
  3. 3.Department of RehabilitationMedical University of WarsawWarsawPoland

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