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Inflammation Research

, Volume 59, Supplement 2, pp 259–261 | Cite as

Histamine in pericarditis of children with congenital heart malformations

  • D. Maslińska
  • M. Laure-Kamionowska
  • P. Rzodkiewicz
  • E. Lukasik-Wojtecka
  • D. Szukiewicz
  • M. Karolczak
  • S. Masliński
Short Communication

Abstract

Introduction

Congenital heart malformations are risk factors that make children susceptible to infections resulting in inflammation.

Material and methods

The concentration of histamine as a modulator of inflammation was quantified in pericardial fluid and expression of histamine H4 receptor (H4R) and histamine-releasing factor (HRF) was determined at mRNA and protein levels. Samples of pericardium and pericardial fluid were obtained during cardiac reconstruction surgery in children.

Results

In children with pericarditis, increased levels of histamine were found and expression of H4R was localized on mast cells. Expression of HRF was independent of presence or absence of inflammation in pericardium and was localized within stationary epithelial cells.

Conclusion

Results indicate that involvement of H4R in pericardial inflammation depends on penetration of mast cells into inflamed tissue, but HRF may not be directly involved in inflammatory reaction of the pericardium.

Keywords

Histamine Pericardium Congenital heart malformations 

References

  1. 1.
    Vonakis BM, Sora R, Langdon JM, Casolaro V, MacDonald SM. Inhibition of cytokine gene transcription by the human recombinant histamine-releasing factor in human T lymphocytes. J Immunol. 2003;171:3742–50.PubMedGoogle Scholar
  2. 2.
    Shore P, Burkhalter A, Cohn W. A method for fluorimetric assay of histamine in tissues. J Pharm Exp Ther. 1959;127:182–6.Google Scholar
  3. 3.
    Maslinska D, Opertowska J, Chabros W, Maslinska M, Grzybowska-Kowalczyk A, Paradowska A, et al. Histamine releasing factor (HRF) in pannus of joints affected by rheumatoid arthritis. Inflamm Res. 2008;57(Suppl):S61–2.CrossRefPubMedGoogle Scholar
  4. 4.
    Jeziorska M, McCollum C, Woolley DE. Mast cell distribution, activation, and phenotype in atherosclerotic lesion of human carotid arteries. J Pathol. 1997;182:115–22.CrossRefPubMedGoogle Scholar
  5. 5.
    Langdon JM, MacDonald SM. Assays for histamine-releasing factors: from indentification and cloning to discovery of binding partners. Methods Mol Biol. 2006;315:231–43.PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag, Basel/Switzerland 2009

Authors and Affiliations

  • D. Maslińska
    • 1
    • 3
  • M. Laure-Kamionowska
    • 1
  • P. Rzodkiewicz
    • 2
  • E. Lukasik-Wojtecka
    • 2
  • D. Szukiewicz
    • 3
  • M. Karolczak
    • 4
  • S. Masliński
    • 2
    • 3
  1. 1.Department of Experimental and Clinical Neuropathology, Mossakowski Medical Research CentrePolish Academy of SciencesWarsawPoland
  2. 2.Department of BiochemistryInstitute of RheumatologyWarsawPoland
  3. 3.Department of PathophysiologyMedical University of WarsawWarsawPoland
  4. 4.2nd Department of Cardiac and General Pediatric SurgeryMedical University of WarsawWarsawPoland

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