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Lipopolysaccharide from Escherichia coli prevents indomethacin-induced gastric damage in rats: role of non-protein sulfhydryl groups and leukocyte adherence

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Abstract

Objective and design

To investigate the role of non-protein sulfhydryl groups (NP-SH) and leukocyte adhesion in the protective effect of lipopolysaccharide (LPS) from Escherichia coli against indomethacin-induced gastropathy.

Materials or subjects

Male Wistar rats were divided into four groups: saline, LPS, saline + indomethacin and LPS + indomethacin, with six rats in each group.

Treatment

Rats were pretreated with LPS (300 μg/kg, by intravenous) or saline. After 6 h, indomethacin was administered (20 mg/kg, by gavage).

Methods

Three hours after treatments, rats were killed. Macroscopic gastric damage, gastric NP-SH concentration, myeloperoxidase (MPO) activity and mesenteric leukocyte adhesion (intravital microscopy) were assessed. Statistical analysis was performed using one-way analysis of variance followed by the Newman–Keuls test. Statistical significance was set at P < 0.05.

Results

LPS reduced the gastric damage, gastric MPO activity and increased gastric NP-SH concentration in indomethacin-induced gastropathy. LPS alone increased gastric NP-SH when compared to saline. Indomethacin increased leukocyte adhesion when compared to the saline, and LPS reduced indomethacin-induced leukocyte adhesion. In addition, LPS alone did not change leukocyte adhesion, when compared to the saline.

Conclusion

LPS protective effect against indomethacin-induced gastropathy is mediated by an increase in the NP-SH and a decrease in leukocyte–endothelial adhesion.

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Abbreviations

LPS:

Lipopolysaccharide

NSAIDs:

Non-steroidal anti-inflammatory drugs

INDO:

Indomethacin

NO:

Nitric oxide

NOS:

Nitric oxide synthase

iNOS:

Inducible nitric oxide synthase

MPO:

Myeloperoxidase

NP-SH:

Non-protein sulfhydryl groups

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Acknowledgments

The authors gratefully acknowledge the technical assistance of Maria Silvandira Freire França. Grants from CNPq (Brazil) supported this work. Dr. Cunha and Dr. Souza are recipients of a CNPq fellowship. This work is part of the requirements to obtain a Master of Science degree in Pharmacology at the School of Medicine, Federal University of Ceara by one of us (A.S. Gomes).

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Authors and Affiliations

  1. Department of Physiology and Pharmacology, School of Medicine, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, Fortaleza, Ceará, 60430-270, Brazil

    Antoniella Souza Gomes, Jand Venes Rolim Medeiros & Marcellus Henrique Loiola Ponte Souza

  2. Department of Pharmacology, Ribeirão Preto School of Medicine, University of Sao Paulo, Ribeirão Prêto, São Paulo, Brazil

    Henrique Paula Lemos & Fernando Queiroz Cunha

Authors
  1. Antoniella Souza Gomes
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  2. Henrique Paula Lemos
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  3. Jand Venes Rolim Medeiros
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  4. Fernando Queiroz Cunha
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  5. Marcellus Henrique Loiola Ponte Souza
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Correspondence to Marcellus Henrique Loiola Ponte Souza.

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Responsible Editor: I. Ahnfelt-Rønne.

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Gomes, A.S., Lemos, H.P., Medeiros, J.V.R. et al. Lipopolysaccharide from Escherichia coli prevents indomethacin-induced gastric damage in rats: role of non-protein sulfhydryl groups and leukocyte adherence. Inflamm. Res. 58, 717–723 (2009). https://doi.org/10.1007/s00011-009-0040-8

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  • DOI: https://doi.org/10.1007/s00011-009-0040-8

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